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Molecular diversity of turncurtoviruses in Iran

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Abstract

Turnip curly top virus (TCTV) is the only member of the newly established genus Turncurtovirus (family Geminiviridae). As part of an ongoing study to identify additional plant hosts and the diversity of turncurtoviruses, between 2012 and 2014, we sampled symptomatic turnip plants and other crops in the provinces Fars and Khorasan Razavi (southern and northeastern Iran, respectively). Infection by turncurtoviruses was tested by PCR and/or rolling-circle amplification (RCA) coupled with restriction enzyme digests. Turncurtoviruses were identified in turnip as well as seven other field crops, including eggplant, basil, radish, lettuce, sugar beet, red beet and spinach. Full turncurtovirus genomes were recovered from 25 of these samples, leading to the identification of TCTV and a new putative turncurtovirus, turnip leaf roll virus (TLRV; 13 isolates), which shares <80 % genome-wide pairwise identity with TCTV. Agroinoculation of plants with an infectious clone of TLRV demonstrated that this virus could infect several plant hosts under greenhouse conditions and could be transmitted by the leafhopper Circulifer haematoceps (Mulsant and Rey, 1855) from agroinoculated to healthy plants.

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Acknowledgments

This study was supported by a grant from Shahid Bahonar University of Kerman, Kerman, Iran, and a block grant from the University of Cape Town, South Africa, awarded to Arvind Varsani. The authors would like to thank Zerina Beslija for excellent technical assistance.

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Authors and Affiliations

  1. Department of Plant Protection, College of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran

    Mehdi Kamali, Jahangir Heydarnejad & Hossain Massumi

  2. Department of Plant Biology, Uppsala BioCenter, Linnean Center of Plant Biology in Uppsala, Swedish University of Agricultural Sciences, Box 7080, 750 07, Uppsala, Sweden

    Anders Kvarnheden

  3. School of Biological Sciences and Biomolecular Interaction Centre, University of Canterbury, Private Bag 4800, Christchurch, 8140, New Zealand

    Simona Kraberger & Arvind Varsani

  4. Department of Plant Pathology and Emerging Pathogens Institute, University of Florida, Gainesville, FL, 32611, USA

    Arvind Varsani

  5. Structural Biology Research Unit, Department of Clinical Laboratory Sciences, University of Cape Town, Rondebosch, 7701, Cape Town, South Africa

    Arvind Varsani

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  1. Mehdi Kamali
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  2. Jahangir Heydarnejad
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Correspondence to Jahangir Heydarnejad.

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Kamali, M., Heydarnejad, J., Massumi, H. et al. Molecular diversity of turncurtoviruses in Iran. Arch Virol 161, 551–561 (2016). https://doi.org/10.1007/s00705-015-2686-6

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  • DOI: https://doi.org/10.1007/s00705-015-2686-6

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