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Molecular characterization of a novel mycovirus from Rhizoctonia fumigata AG-Ba isolate C-314 Baishi

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Abstract

The complete genome sequence of a novel dsRNA virus isolated from Rhizoctonia fumigata AG-Ba isolate C-314 Baishi (designated as Rhizoctonia fumigata virus 1, RfV1) was determined. The RfV1 genome was 9,907 bp in length and contained two open reading frames (ORFs). ORF1 potentially coded for a 198.10-kDa protein (P1). P1 shared low but significant amino acid sequence similarity to the putative protein encoded by Lentinula edodes mycovirus (LeV) ORF1. P1 contained a NUDIX domain, which was also present in the putative proteins encoded by the ORF1s of LeV and Phlebiopsis gigantea large virus 1 (PgLV-1). ORF2 potentially coded for a 146.72-kDa protein (P2) that contained the conserved motifs of the RNA-dependent RNA polymerase (RdRp). ORF1 and ORF2 were overlapping, and it was predicted that ORF2 could be translated as a fusion with ORF1 via a ribosomal -1 frameshifting mechanism. Phylogenetic analysis indicated that RfV1 clustered with PgLV-1, LeV and Rosellinia necatrix megabirnavirus 1 (RnMBV1) in a separate clade independent of other virus genera. We propose that RfV1, along with PgLV-1 and LeV, should be grouped into a new viral genus related to the family Megabirnaviridae. This is the first report of the full-length genome sequence of a novel mycovirus isolated from R. fumigata.

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Acknowledgements

The authors would like to thank Dr. Akira Ogoshi, Hokkaido University, Japan, for kindly providing the Rhizoctonia fumigata AG-Ba isolate C-314 Baishi in this study, and Dr. Bryce Falk, University of California, Davis, for critically reviewing of the manuscript. This research was supported by a grant-in-aid from Yunnan Tobacco Company (2014YN10), the National Natural Scientific Foundation of China (30660006, 31160352), and the National Basic Research Program (2011CB100400).

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Authors and Affiliations

  1. Yunnan Agricultural University, Kunming, 650201, Yunnan, China

    Yanqiong Li, Ping Xu, Lifang Zhang & Genhua Yang

  2. Yunnan Academy of Tobacco Agricultural Sciences, Kunming, 650021, Yunnan, China

    Yanqiong Li, Ping Xu, Lifang Zhang, Zhenyuan Xia, Xiyun Qin & Xiaohan Mo

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  1. Yanqiong Li
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  2. Ping Xu
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  3. Lifang Zhang
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  7. Xiaohan Mo
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Correspondence to Genhua Yang or Xiaohan Mo.

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Li, Y., Xu, P., Zhang, L. et al. Molecular characterization of a novel mycovirus from Rhizoctonia fumigata AG-Ba isolate C-314 Baishi. Arch Virol 160, 2371–2374 (2015). https://doi.org/10.1007/s00705-015-2483-2

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  • DOI: https://doi.org/10.1007/s00705-015-2483-2

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