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The complete nucleotide sequence of chrysanthemum stem necrosis virus

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Abstract

The complete genome sequence of chrysanthemum stem necrosis virus (CSNV) was determined using Roche 454 next-generation sequencing. CSNV is a tentative member of the genus Tospovirus within the family Bunyaviridae, whose members are arthropod-borne. This is the first report of the entire RNA genome sequence of a CSNV isolate. The large RNA of CSNV is 8955 nucleotides (nt) in size and contains a single open reading frame of 8625 nt in the antisense arrangement, coding for the putative RNA-dependent RNA polymerase (L protein) of 2874 aa with a predicted Mr of 331 kDa. Two untranslated regions of 397 and 33 nt are present at the 5’ and 3’ termini, respectively. The medium (M) and small (S) RNAs are 4830 and 2947 nt in size, respectively, and show 99 % identity to the corresponding genomic segments of previously partially characterized CSNV genomes. Protein sequences for the precursor of the Gn/Gc proteins, N and NSs, are identical in length in all of the analysed CSNV isolates.

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Acknowledgments

This work was performed in the Seventh Framework Programme FP7 in project KBBE-2008-1-4-01 (QBOL) No. 226482 funded by the European Commission.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Plant Research International, Wageningen University and Research Centre, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands

    A. M. Dullemans & R. A. A. van der Vlugt

  2. National Plant Protection Organization, P.O. Box 9102, 6700 HC, Wageningen, The Netherlands

    J. Th. J. Verhoeven

  3. Laboratory of Virology, Wageningen University, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands

    R. Kormelink

Authors
  1. A. M. Dullemans
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  2. J. Th. J. Verhoeven
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  3. R. Kormelink
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  4. R. A. A. van der Vlugt
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Corresponding author

Correspondence to A. M. Dullemans.

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Dullemans, A.M., Verhoeven, J.T.J., Kormelink, R. et al. The complete nucleotide sequence of chrysanthemum stem necrosis virus. Arch Virol 160, 605–608 (2015). https://doi.org/10.1007/s00705-014-2282-1

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  • DOI: https://doi.org/10.1007/s00705-014-2282-1

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