Abstract
The complete genome sequence of a new virus isolated from a motherwort plant exhibiting yellow mottle, mild mosaic, and stunting symptoms in Andong, Korea, was determined. The genome of this virus is composed of two single-stranded RNAs (7068 and 4963 nucleotides in length, respectively) carrying poly(A) tails. RNA1 contains one large open reading frame (RNA1-ORF1), while two potential ORFs (RNA2-ORF1 and RNA2-ORF2) were found in RNA2. BLAST searches of protein databases showed that RNA1-ORF1 and RNA2-ORF2 have maximum amino acid sequence identities of 53 % and 57 % to the RNA1-ORF1 and RNA2-ORF2, respectively, of lettuce necrotic leaf curl virus (LNLCV, a recently identified torradovirus). Phylogenetic analysis provided further evidence that the virus identified in this study is probably a member of a new species in the genus Torradovirus. The name “motherwort yellow mottle virus” (MYMoV) is proposed for this new virus.
Similar content being viewed by others
References
Adams MJ, King AM, Carstens EB (2013) Ratification vote on taxonomic proposals to the International Committee on Taxonomy of Viruses (2013). Arch Virol 158:2023–2030
Barbosa JC, Eckstein B, Bergamin Filho A, Rezende JAM, Dallagnol LJ (2013) First report of tomato yellow spot virus infecting Leonurus sibiricus in Brazil. Plant Dis 97:289
Batuman O, Kuo YW, Palmieri M, Rojas MR, Gilbertson RL (2010) Tomato chocolate spot virus, a member of a new torradovirus species that causes a necrosis-associated disease of tomato in Guatemala. Arch Virol 155:857–869
Islam MA, Ahmed F, Das AK, Bachar SC (2005) Analgesic and anti-inflammatory activity of Leonurus sibiricus. Fitoterapia 76:359–362
Schelhorn SE, Fischer M, Tolosi L, Altmuller J, Nurnberg P, Pfister H, Lengauer T, Berthold F (2013) Sensitive detection of viral transcripts in human tumor transcriptomes. PLoS Comput Biol 9:e1003228
Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739
Turina M, Ricker MD, Lenzi R, Masenga V, Ciuffo M (2007) A severe disease of tomato in the Culiacan area (Sinaloa, Mexico) is caused by a new picorna-like viral species. Plant Dis 91:932–941
Verbeek M, Dullemans AM, van den Heuvel JF, Maris PC, van der Vlugt RA (2007) Identification and characterisation of tomato torrado virus, a new plant picorna-like virus from tomato. Arch Virol 152:881–890
Verbeek M, Dullemans AM, van den Heuvel JF, Maris PC, van der Vlugt RA (2008) Tomato marchitez virus, a new plant picorna-like virus from tomato related to tomato torrado virus. Arch Virol 153:127–134
Verbeek M, Dullemans A, van den Heuvel H, Maris P, van der Vlugt R (2010) Tomato chocolate virus: a new plant virus infecting tomato and a proposed member of the genus Torradovirus. Arch Virol 155:751–755
Verbeek M, Dullemans AM, van Raaij HM, Verhoeven JT, van der Vlugt RA (2014) Lettuce necrotic leaf curl virus, a new plant virus infecting lettuce and a proposed member of the genus Torradovirus. Arch Virol 159:801–805
Acknowledgments
This research was supported by a grant from the Agenda Program (PJ008841) funded by the Rural Development Administration of Korea.
Author information
Authors and Affiliations
Corresponding authors
Rights and permissions
About this article
Cite this article
Seo, JK., Kang, M., Kwak, HR. et al. Complete genome sequence of motherwort yellow mottle virus, a novel putative member of the genus Torradovirus . Arch Virol 160, 587–590 (2015). https://doi.org/10.1007/s00705-014-2254-5
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00705-014-2254-5