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Comparison of two immunoassays for quantification of hepatitis B surface antigen in Chinese patients with concomitant hepatitis B surface antigen and hepatitis B surface antibodies

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Abstract

Two commercial quantitative immunoassay platforms are currently available for the detection of hepatitis B surface antigen (HBsAg) within a wide range of concentrations: the Architect HBsAg assay and the Elecsys HBsAg II. However, there are limited data concerning their performance in patients who are positive for both HBsAg and anti-HBs antibodies. In the present study, 127 serum samples from Chinese patients with hepatitis B carrying both HBsAg and anti-HBs antibodies were analyzed. HBsAg levels measured in parallel using the Elecsys HBsAg II and Architect HBsAg assay were compared. There was a significant correlation between HBsAg levels measured by Elecsys HBsAg II and Architect HBsAg assay (correlation coefficient, r = 0.992, P < 0.05). Bland-Altman analysis indicated only minor discordance between the methods (mean difference between Elecsys HBsAg II and Architect HBsAg assay, −0.02 log10 IU/ml; 95 % confidence interval, −0.40–0.37 log10 IU/ml). In conclusion, the results of the quantitative Elecsys HBsAg II were highly correlated with those of the Architect HBsAg assay in patients carrying both HBsAg and anti-HBs antibodies, but differences were observed between the platforms in samples with low HBsAg levels.

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Acknowledgments

This work was supported by a grant from the Outstanding Young Talent Plan of Shanghai (XYQ2013095), Pandeng Talent Plan of the Shanghai Tenth People’s Hospital (2013), Fudan University Zhuoxue Talent Plan (2011), the National Science Foundation (Grant No. 81370067), and a grant from Shanghai Shenkang Hospital Development Center (Grant No. SHDC22014006).

Conflict of interest

None declared.

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Authors and Affiliations

  1. Department of Laboratory Medicine, Shanghai Tenth People’s Hospital, Tongji University, Shanghai, 200072, China

    Weiwei Liu & Yannan Yang

  2. Department of Laboratory Medicine, Shanghai Skin Disease Hospital, Shanghai, 200071, China

    Weiwei Liu & Yannan Yang

  3. Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, 200040, China

    Yao Hu & Tingting Hu

  4. Department of Infectious Diseases, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, 200040, China

    Xinyu Wang

Authors
  1. Weiwei Liu
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  2. Yao Hu
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  3. Yannan Yang
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  4. Tingting Hu
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  5. Xinyu Wang
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Corresponding authors

Correspondence to Weiwei Liu or Xinyu Wang.

Additional information

W. Liu, Y. Hu and Y. Yang contributed equally to this work.

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Liu, W., Hu, Y., Yang, Y. et al. Comparison of two immunoassays for quantification of hepatitis B surface antigen in Chinese patients with concomitant hepatitis B surface antigen and hepatitis B surface antibodies. Arch Virol 160, 191–198 (2015). https://doi.org/10.1007/s00705-014-2253-6

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  • DOI: https://doi.org/10.1007/s00705-014-2253-6

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