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Identification and complete genome analysis of kobuvirus in faecal samples of European roller (Coracias garrulus): for the first time in a bird

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Abstract

The genus Kobuvirus (Picornaviridae) consists of three species, Aichivirus A (e.g., Aichi virus, which infects humans), Aichivirus B and Aichivirus C. Kobuvirus have not been detected in non-mammal species including birds. In this study, a novel kobuvirus was identified in 3 (17 %) out of 18 faecal samples collected from European rollers (Coracias garrulus) in Hungary. The complete genome sequence of strain SZAL6-KoV/2011/HUN (KJ934637), which was determined using a novel 5′/3′ RACE method (dsRNA-RACE) involving a double-stranded (ds)RNA intermediate, has a type-V IRES at the 5′ end and a cis-acting element (CRE) in the 3C gene and encodes L and 2AH-box/NC proteins, but it does not contain the sequence forming a “barbell-like” secondary RNA structure in the 3′UTR. SZAL6-KoV/2011/HUN has 72 %, 73 %, and 81 % amino acid sequence identity to the P1, P2, and P3 protein, respectively, of Aichi virus. Evolutionary analysis showed that SZAL6-KoV/2011/HUN shares a common ancestor with other kobuviruses but belongs to a more ancient lineage in the species Aichivirus A. Investigation of the known kobuviruses in different animals and discovery of novel kobuviruses in potential host species helps to clarify the evolutionary connection and zoonotic potential of kobuviruses.

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Acknowledgments

This work was supported by grants from the Hungarian Scientific Research Fund (OTKA K83013 and OTKA K111615) and TÁMOP 4.2.4.A/ 2-11/1-2012-0001 ‘National Excellence Program’. We thank Attila Hunor Török, Béla Tokody (supported by the Conservation Management and Animal Health Monitoring of Natura 2000 Bird Species HU-SRB IPA CBC PROJECT), and Edit Pollák for the collection of faecal samples, and Gábor Földvári for control specimens from Myodes spp. Special thanks to Michael Antony Garay for proofreading the manuscript.

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Regional Laboratory of Virology, National Reference Laboratory of Gastroenteric Viruses, ÁNTSZ Regional Institute of State Public Health Service, Szabadság út 7, 7623, Pécs, Hungary

    Péter Pankovics, Ákos Boros & Gábor Reuter

  2. Hungarian Ornithological and Nature Conservation Society, Budapest, Hungary

    Tamás Kiss

Authors
  1. Péter Pankovics
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  2. Ákos Boros
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  3. Tamás Kiss
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  4. Gábor Reuter
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Corresponding author

Correspondence to Gábor Reuter.

Additional information

Nucleotide sequence data reported are available in the GenBank database under accession number KJ934637.

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Pankovics, P., Boros, Á., Kiss, T. et al. Identification and complete genome analysis of kobuvirus in faecal samples of European roller (Coracias garrulus): for the first time in a bird. Arch Virol 160, 345–351 (2015). https://doi.org/10.1007/s00705-014-2228-7

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  • DOI: https://doi.org/10.1007/s00705-014-2228-7

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