Skip to main content
SpringerLink
Log in

The complete genome sequence of New World jatropha mosaic virus

  • Annotated Sequence Record
  • Published:
Archives of Virology Aims and scope Submit manuscript

Abstract

Full-length sequences of a bipartite begomovirus were obtained from a plant of Jatropha multifida in Florida showing symptoms of foliar mosaic, distortion and necrosis. Sequences of four clones each of a DNA-A and DNA-B were obtained, which showed very low sequence diversity among themselves. The clones were infectious when biolistically inoculated to J. multifida, Phaseolus vulgaris and Nicotiana tabacum, but not to J. curcas. The DNA-A sequences had less than 89 % pairwise identity scores with the DNA-A of other begomoviruses. The DNA-A appeared to be a recombinant in that 18 % of the DNA-A (470 nt) had a pairwise identity score of 91.98 % with RhRGMV, indicating that this portion most likely originated from a virus closely related to RhRGMV. The remaining 82 % of the DNA-A had lower identity scores with TbMoLCV (87.84 %) and RhRGMV (87.46 %), which suggests that this part of the component originated from an undescribed virus. There was no evidence for recombination in the DNA-B. Equivalent sequences of the DNA-A had the highest identity score (94.18 %) with a 533-nt sequence obtained from J. multifida from Puerto Rico in 2001 (GenBank accession no. AF058025). Pairwise comparison, recombination and phylogenetic analysis, and biology suggest that these clones are those of jatropha mosaic virus first reported from Puerto Rico. This is the first report of the complete genome sequence of jatropha mosaic virus.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. Achten WMJ, Verchot L, Franken YJ, Mathijs E, Singh VP, Aerts R, Muys B (2008) Jatropha bio-diesel production and use. Biomass Bioenergy 32:1063–1084

    Article  CAS  Google Scholar 

  2. Albuquerque LC, Martin DP, Avila AC, Inoue-Nagata AK (2010) Characterization of tomato yellow vein streak virus, a begomovirus from Brazil. Virus Genes 40:140–147

    Article  PubMed  CAS  Google Scholar 

  3. Akad F, Webb S, Nyoike W, Liburd OE, Turechek W, Adkins S, Polston JE (2008) Detection of cucurbit leaf crumple virus in Florida cucurbits. Plant Dis 92:648

    Article  Google Scholar 

  4. Aswatha Narayana DS, Rangaswamy KT, Shankarappa KS, Maruthi MN, Lakshminarayana Reddy CN, Rekha AR, Keshava Murthy KV (2007) Distinct begomoviruses closely related to Cassava Mosaic Viruses cause Indian Jatropha Mosaic Disease. Int J Virol 3:1–11

    Article  Google Scholar 

  5. Bird J, Sanchez J, Rodriguez RL, Julia FJ (1975) Rugaceous (whitefly-transmitted) viruses in Puerto Rico. In: Bird J, Maramorosch K (eds) Tropical diseases of legumes. Academic Press, Inc., New York, NY, pp 3–26

    Chapter  Google Scholar 

  6. Brown JK, Idris AM, Torres-Jerez I, Banks GK, Wyatt SD (2001) The core region of the coat protein gene is highly useful for establishing the provisional identification and classification of begomoviruses. Arch Virol 146:1581–1598

    Article  PubMed  CAS  Google Scholar 

  7. Gao S, Qu J, Chua NH, Ye J (2010) A new strain of Indian cassava mosaic virus causes a mosaic disease in the biodiesel crop Jatropha curcas. Arch Virol 155:607–612

    Article  PubMed  CAS  Google Scholar 

  8. Guenoune-Gelbart D, Sufrin-Ringwald T, Capobianco H, Gaba V, Polston JE, Lapidot M (2010) Inoculation of plants with begomoviruses by particle bombardment without cloning: using rolling-circle amplification of total DNA from infected plants. J Virol Methods 168:87–93

    Article  PubMed  CAS  Google Scholar 

  9. Heller J (1996) Physic nut. Jatropha curcas L. Promoting the conservation and use of underutilized and neglected crops. 1. Institute of Plant Genetics and Crop Plant Research, Gatersleben/International Plant Genetic Resources Institute, Rome

  10. Kashina BD, Alegbejo MD, Banwo OO, Nielsen SL, Nicolaisen M (2013) Molecular identification of a new begomovirus associated with mosaic disease of Jatropha curcas L. in Nigeria. Arch Virol 158:511–514

    Article  PubMed  CAS  Google Scholar 

  11. Lima AT, Sobrinho RR, González-Aguilera J, Rocha CS, Silva SJ, Xavier CA, Silva FN, Duffy S, Zerbini FM (2013) Synonymous site variation due to recombination explains higher genetic variability in begomovirus populations infecting non-cultivated hosts. J Gen Virol 94:418–431

    Article  PubMed  CAS  Google Scholar 

  12. Martin DP, Rybicki EP (2000) RDP: detection of recombination amongst aligned sequences. Bioinformatics 16:562–563

    Article  PubMed  CAS  Google Scholar 

  13. Martinez Y (2008) Emergence of begomoviruses in Cuba. Rev Protección Veg 23:11–15

    Google Scholar 

  14. Muhire B, Martin DP, Brown JK, Navas-Castillo J, Moriones E, Zerbini FM, Rivera-Bustamante R, Malathi VG, Briddon RW, Varsani A (2013) A genome-wide pairwise-identity-based proposal for the classification of viruses in the genus Mastrevirus (family Geminiviridae). Arch Virol 158:1411–1424

    Article  PubMed  CAS  Google Scholar 

  15. Polston JE, Capobianco H (2013) Transmitting plant viruses using whiteflies. J Vis Exp 81:e4332. doi:10.3791/4332

    PubMed  Google Scholar 

  16. Polston JE, Hiebert E, McGovern RJ, Stansly PA, Schuster DJ (1993) Host range of Tomato mottle virus, a new geminivirus infecting tomato in Florida. Plant Dis 77:1181–1184

    Article  Google Scholar 

  17. Raj SK, Snehi SK, Kumar S, Khan MS, Pathre U (2008) First molecular identification of a begomovirus in India that is closely related to Cassava mosaic virus and causes mosaic and stunting of Jatropha curcas L. Australas Plant Dis Notes 3:69–71

    Article  CAS  Google Scholar 

  18. Ramkat RC, Calari A, Maghuly F, Laimer M (2011) Biotechnological approaches to determine the impact of viruses in the energy crop plant Jatropha curcas. Virol J 8:386–395

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  19. Rojas MR, Gilbertson RL, Russell DR, Maxwell DP (1993) Use of degenerate primers in the polymerase chain reaction to detect whitefly-transmitted geminiviruses. Plant Dis 77:340–347

    Article  CAS  Google Scholar 

  20. Roye ME, Collins AM, Maxwell DP (2006) First report of a begomovirus associated with the common weed Jatropha gossypifolia in Jamaica. Plant Pathol 55:286

    Article  Google Scholar 

  21. Sabandar CW, Ahmat N, Jaafar FM, Sahidin I (2013) Medicinal property, phytochemistry and pharmacology of several Jatropha species (Euphorbiaceae): a review. Phytochemistry 85:7–29

  22. Tamura K, Nei M (1993) Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol 10:512–526

    PubMed  CAS  Google Scholar 

  23. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 10:2731–2739

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Plant Pathology, University of Florida, Gainesville, FL, 32601, USA

    J. E. Polston, M. A. Londoño & H. Capobianco

Authors
  1. J. E. Polston
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. A. Londoño
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. H. Capobianco
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to J. E. Polston.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Suppl. Fig. 1 Genomic map of DNA-A and DNA-B clones obtained from J. multifida from Florida showing in gray tones the relative locations of the partial sequences obtained from J. gossypifolia from Cuba (DQ207807), J. gossypifolia from Jamaica (AF324410) and J. multifida from Puerto Rico (AF058025). Open reading frames of the begomovirus are shown in teal.

Supplementary material 1 (TIFF 812 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Polston, J.E., Londoño, M.A. & Capobianco, H. The complete genome sequence of New World jatropha mosaic virus. Arch Virol 159, 3131–3136 (2014). https://doi.org/10.1007/s00705-014-2132-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-014-2132-1

Keywords

Search

Navigation