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Molecular characterization of a Chinese isolate of potato virus A (PVA) and evidence of a genome recombination event between PVA variants at the 3′-proximal end of the genome

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Abstract

Potato plants that exhibited mosaic symptoms were collected in Xiangxi, Hunan province, China. Multiplex RT-PCR screening for common viruses revealed the presence of potato virus A (PVA) in these samples. ELISA with virus-specific antibodies confirmed infection by PVA in the plants. Rod-shaped virions of ~750 nm in length and ~13 nm in width were observed by transmission electron microscopy. One virus isolate (designated PVA-Hunan) was subjected to molecular characterization. The viral genome consisted of 9,567 nucleotides, excluding the poly(A) tail, and encoded a polyprotein of 3,059 amino acids. A second characteristic potyvirus open reading frame (ORF), pretty interesting Potyviridae ORF (pipo), was located at nucleotides 2,834-3,139. The isolate shared 84 % to 98 % and 93 % to 99 % sequence identity with other PVA isolates at the nucleotide and amino acid level, respectively. Phylogenetic analysis demonstrated that, within the PVA group, PVA-Hunan clustered most closely with the Finnish isolate Her, then with isolates 143, U, Ali, M and B11. The isolate TamMV stood alone at a separate branch. However, scanning of complete genome sequences using SimPlot revealed 99 %-sequence identity between PVA-Hunan and TamMV in the 3′-proximal end of the genome (~nt 9,160 to the 3′end) and a 50 %-94 % (average ~83 %) identity upstream of nt 9,160. In contrast, 98 % identity between PVA-Hunan and isolates M and B11 was detected for nucleotides 1 to ~9,160, but only ~94 % for the 3′-proximal region, suggesting a genome recombination event (RE) at nt 9,133. The recombination breakpoint also was identified by the Recombination Detection Program (RDP). The RE was further confirmed by analysis of the CP gene, where the apparent RE was located.

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Acknowledgments

This research was supported by the National High Technology Research and Development Program (Project no. 2013AA102603), Hunan Provincial Natural Science Foundation (Project no. 13JJ2029), and the Ministry of Science and Technology of China through the ISTP Canada-China Science and Technology program (Project no. CCRD07-077). The authors thank Virginia Dickson for careful proofreading of the manuscript prior to submission.

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Authors and Affiliations

  1. Hunan Provincial Key Laboratory of Crop Germplasm Innovation and Utilization, Hunan Provincial Engineering Research Center for Potatoes, College of Horticulture and Landscape, Hunan Agricultural University, Changsha, 410128, Hunan, China

    Changzheng He, Wei Zhang, Xinxi Hu & Xingyao Xiong

  2. Agricultural Certification Services, 1030 Lincoln Road, Fredericton, NB, E3B 8B7, Canada

    Mathuresh Singh

  3. Potato Research Centre, Agriculture and Agri-Food Canada, 850 Lincoln Road, P.O. Box 20280, Fredericton, NB, E3B 4Z7, Canada

    Xianzhou Nie

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  1. Changzheng He
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  2. Wei Zhang
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  3. Xinxi Hu
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  4. Mathuresh Singh
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  5. Xingyao Xiong
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  6. Xianzhou Nie
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Correspondence to Xingyao Xiong or Xianzhou Nie.

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C. He, W. Zhang are co-first authors.

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He, C., Zhang, W., Hu, X. et al. Molecular characterization of a Chinese isolate of potato virus A (PVA) and evidence of a genome recombination event between PVA variants at the 3′-proximal end of the genome. Arch Virol 159, 2457–2462 (2014). https://doi.org/10.1007/s00705-014-2053-z

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