Abstract
The measurement of neutralizing antibodies induced by the glycoprotein of rabies virus is indispensable for assessing the level of neutralizing antibodies in animals or humans. A rapid fluorescent focus inhibition test (RFFIT) has been approved by WHO and is the most widely used method to measure the virus-neutralizing antibody content in serum, but a rapid test system would be of great value to screen large numbers of serum samples. To develop and evaluate a latex agglutination test (LAT) for measuring rabies virus antibodies, a recombinant glycoprotein was expressed in an insect cell system and purified, and the protein was coated onto latex beads at concentrations of 0.1, 0.25, 0.5, 0.75, and 1 mg/ml to find out the optimal concentration for coating latex beads. It was found that 0.5 mg/ml of recombinant protein was optimal for coating latex beads, and this concentration was used to sensitize the latex beads for screening of dog serum samples. Grading of LAT results was done with standard reference serum with known antibody titers. A total of 228 serum samples were tested, out of which 145 samples were positive by both RFFIT and LAT, and the specificity was found to be 100 %. In RFFIT, 151 samples were positive, the sensitivity was found to be 96.03 %, and the accuracy/concordance was found to be 97.39 %. A rapid field test—a latex agglutination test (LAT)—was developed and evaluated for rabies virus antibody assessment using recombinant glycoprotein of rabies virus expressed in an insect cell system.
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References
Webster LT, Dawson JR (1935) Early diagnosis of rabies by mouse inoculation: measurement of host immunity to rabies by mouse protection test. Proc Soc Exp Biol Med 32:570–573
Smith JS, Yager PA, Baer GM (1996) In: Meslin FX, Kaplan MM, Koprowski H (eds) Laboratory techniques in rabies, 4th edn. WHO, Geneva, pp 181–192
Cliquet F, Aubert M, Sagne L (1998) Development of a fluorescent antibody virus neutralization test (FAVN test) for the quantification of rabies-neutralizing antibody. J Immunol Methods 212:79–87
Atanasiu P, Rollin P, Delagneau JF (1979) Use of enzyme immune-assay protein A for rabies antigen and antibody determination. Dev Biol Stand 46:207–215
Muhamuda K, Madhusudana SN, Ravi V (2007) Development and evaluation of a competitive ELISA for estimation of rabies neutralizing antibodies after post-exposure rabies vaccination in humans. Intl J Infect Dis 11:441–445
Seiji S, Kazuaki M, Takashi M, Kentaro Y, Takehito Y, Katsuyoshi T, Yukuharu K, Pakamatz K, Kazuyo G, Kamruddin A, Hidekatsu I, Akira N (2009) Development and evaluation of a rapid neutralizing antibody test for rabies. J Virol Methods 161:58–62
Hualei W, Na F, Songtao Y, Chengyu W, Tiecheng W, Yuwei G, Jianqing S, Xuexing Z, Xiaoqiang H, Hainan H, Ruimei Y, Xiaohuan Z, Geng H, Xianzhu X (2010) A rapid immunochromatographic test strip for detecting rabies virus antibody. J Virol Methods 170:80–85
Kasempimolporn S, Saengseesom W, Lumlertdacha B, Sitprija V (2000) Detection of rabies antibodies. J Clin Microbiol 38:3098–3099
Madhusudana SN, Saraswathi S (2003) Development and evaluation of a latex agglutination test for rabies antibodies. J Clin Virol 27:129–135
Reed LJ, Muench H (1938) A simple method of estimating fifty percent end points. Am J Hyg 27:493–497
Lengyel A, Adam E, Nasz I (1993) Latex agglutination and adenoviruses. II Detection of antigens. Acta Microb Hung 40:85–90
Cox JH, Dietzschold B, Schneider LG (1977) Rabies virus glycoprotein II. Biological and serological characterization. Infect Immun 16:754–759
Borras-Cuesta F, Petit-Camurdan A, Fedon Y (1987) Engineering of immunogenic peptides by co-linear synthesis of determinants recognized by band T cells. Eur J Immunol 17:1213–1215
Fair BD, Jamieson AM (1980) Studies of protein adsorption on polystyrene latex surfaces. J Coll Inter Sci 77:525–534
Hadfield SG, Lane A, Mcllmurray MB (1987) A novel colored latex test for the detection and identification of more than one antigen. J Immunol Methods 97:153–158
OIE Terrestrial Manual (2013) Manual of standards for diagnostic tests and vaccines. Off Int Des Epizoot 280–291
Susan ES, Cathleen H, William W, Michael N, Jesse B, Josh S, Kirk RJ, Robert BM, Gregory JB, William DT, Rupprecht CE, Donna MA (2007) Identification and characterization of a human monoclonal antibody that potently neutralizes a broad panel of rabies virus isolates. Vaccine 25:2800–2810
World Health Organization (1991) WHO expert committee on rabies. 8th report WHO Tech Rev Seri No 824 Geneva WHO, pp 21–6
Saengseesom W, Kasempimolporn S, Akesowan S, Ouisuwan S, Sitprija V (2010) Use of latex agglutination test to determine rabies antibodies in production of rabies antisera in horses. WHO 41:1387–1392
Hughes JH, Tuomari AV, Mann DR, Hamparian VV (1984) Latex immunoassay for rapid detection of Rotavirus. J Clin Microbiol 20:441–447
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We wish to thank the Vice Chancellor and Registrar of TANUVAS, Chennai, South India, for financial support.
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Jemima, E.A., Manoharan, S. & Kumanan, K. Development and evaluation of a recombinant-glycoprotein-based latex agglutination test for rabies virus antibody assessment. Arch Virol 159, 1987–1993 (2014). https://doi.org/10.1007/s00705-014-2033-3
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DOI: https://doi.org/10.1007/s00705-014-2033-3