Brief Report

Archives of Virology

, Volume 159, Issue 3, pp 567-572

Open Access This content is freely available online to anyone, anywhere at any time.

A giant Pseudomonas phage from Poland

  • Zuzanna Drulis-KawaAffiliated withInstitute of Genetics and Microbiology, University of Wroclaw Email author 
  • , Tomasz OlszakAffiliated withInstitute of Genetics and Microbiology, University of Wroclaw
  • , Katarzyna DanisAffiliated withInstitute of Genetics and Microbiology, University of Wroclaw
  • , Grazyna Majkowska-SkrobekAffiliated withInstitute of Genetics and Microbiology, University of Wroclaw
  • , Hans-W. AckermannAffiliated withDepartment of Microbiology-Infectiology and Immunology, Medical School, Laval University, Quebec, 1050

Abstract

A novel giant phage of the family Myoviridae is described. Pseudomonas phage PA5oct was isolated from a sewage sample from an irrigated field near Wroclaw, Poland. The virion morphology indicates that PA5oct differs from known giant phages. The phage has a head of about 131 nm in diameter and a tail of 136 × 19 nm. Phage PA5oct contains a genome of approximately 375 kbp and differs in size from any tailed phages known. PA5oct was further characterized by determination of its latent period and burst size and its sensitivity to heating, chloroform, and pH.

Keywords

Bacteriophage DNA size Giant Myoviridae Pseudomonas