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Genomic sequence of an infectious bursal disease virus isolate from Zambia: classical attenuated segment B reassortment in nature with existing very virulent segment A

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Abstract

We determined the complete nucleotide sequence of an infectious bursal disease (IBD) virus (IBDV) isolate (designated KZC-104) from a confirmed IBD outbreak in Lusaka in 2004. The genome consisted of 3,074 and 2,651 nucleotides in the coding regions of segments A and B, respectively. Alignment of both nucleotide and deduced amino acid sequences and phylogenetic analysis revealed that the genome segment A of KZC-104 was derived from a very virulent (VV) strain, whereas its segment B was derived from a classical attenuated strain. On BLAST search, the full-length segment A and B sequences showed 98 % nucleotide sequence identity to the VV strain D6948 and 99.8 % nucleotide sequence identity to the classical attenuated strain D78. This is a unique IBDV reassortant strain that has emerged in nature, involving segment B of a cell-culture-adapted attenuated vaccine.

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References

  1. Boot HJ, ter Huurne AA, Hoekman AJ, Peeters BP, Gielkens AL (2000) Rescue of very virulent and mosaic infectious bursal disease virus from cloned cDNA: VP2 is not the sole determinant of the very virulent phenotype. J Virol 74:6701–6711

    Article  PubMed  CAS  Google Scholar 

  2. Cosgrove AS (1962) An apparently new disease of chickens—avian nephrosis. Avian Dis 9:385–389

    Article  Google Scholar 

  3. Da Costa B, Chevalier C, Henry C, Huet JC, Petit S, Lepault J, Boot H, Delmas B (2002) The capsid of infectious bursal disease virus contains several small peptides arising from the maturation process of pVP2. J Virol 76:2393–2402

    Article  PubMed  Google Scholar 

  4. Gao HL, Wang XM, Gao YL, Fu CY (2007) Direct evidence of reassortment and mutant spectrum analysis of a very virulent infectious bursal disease virus. Avian Dis 51(4):893–899

    Article  PubMed  Google Scholar 

  5. Ismail NM, Saif YM, Moorhead PD (1988) Lack of pathogenicity of five serotype 2 infectious bursal disease viruses in chickens. Avian Dis 32:757–759

    Article  PubMed  CAS  Google Scholar 

  6. Jackwood DJ, Sommer-Wagner SE, Crossley BM, Stoute ST, Woolcock PR, Charlton BR (2011) Identification and pathogenicity of a natural reassortant between a very virulent serotype 1 infectious bursal disease virus (IBDV) and a serotype 2 IBDV. Virology 420(2):98–105

    Google Scholar 

  7. Kasanga CJ, Yamaguchi T, Wambura PN, Munang’andu HM, Ohya K, Fukushi H (2008) Detection of infectious bursal disease virus (IBDV) genome in free-living pigeon and guinea fowl in Africa suggests involvement of wild birds in the epidemiology of IBDV. Virus Genes 36:521–529

    Article  PubMed  CAS  Google Scholar 

  8. Lejal N, Da Costa B, Huet JC, Delmas B (2000) Role of Ser-652 and Lys-692 in the protease activity of infectious bursal disease virus VP4 and identification of its substrate cleavage sites. J Gen Virol 81:983–992

    PubMed  CAS  Google Scholar 

  9. Le Nouën C, Rivallan G, Toquin D, Darlu P, Morin Y, Beven V, de Boisseson C, Cazaban C, Comte S, Gardin Y, Eterradossi N (2006) Very virulent infectious bursal disease virus: reduced pathogenicity in a rare natural segment-B-reassorted isolate. J Gen Virol 87:209–216

    Article  PubMed  Google Scholar 

  10. Petkov D, Linnemann E, Kapczynski DR, Sellers HS (2007) Full-length sequence analysis of four IBDV strains with different pathogenicities. Virus Genes 34:315–326

    Article  PubMed  CAS  Google Scholar 

  11. Shwed PS, Dobos P, Cameron LA, Vakharia VN, Duncan R (2002) Birnavirus VP1 proteins form a distinct subgroup of RNA-dependent RNA polymerases lacking a GDD motif. Virology 296:241–250

    Article  PubMed  CAS  Google Scholar 

  12. Wei Y, Li J, Zheng J, Xu H, Li L, Yu L (2006) Genetic reassortment of infectious bursal disease virus in nature. Biochem Biophys Res Commun 350:277–287

    Article  PubMed  CAS  Google Scholar 

  13. Wei Y, Yu X, Zheng J, Chu W, Xu H, Yu X, Yu L (2008) Reassortant infectious bursal disease virus isolated in China. Virus Res 131(2):279–282

    Article  PubMed  CAS  Google Scholar 

  14. Wu CC, Rubinelli P, Lin TL (2007) Molecular detection and differentiation of infectious bursal disease virus. Avian Dis 51:515–526

    Article  PubMed  Google Scholar 

  15. Xia RX, Wang HY, Huang GM, Zhang MF (2008) Sequence and phylogenetic analysis of a Chinese very virulent infectious bursal disease virus. Arch Virol 153(9):1725–1729

    Article  PubMed  CAS  Google Scholar 

  16. Yamaguchi T, Ogawa M, Miyoshi M, Inoshima Y, Fukushi H, Hirai K (1997) Sequence and phylogenetic analyses of highly virulent infectious bursal disease virus. Arch Virol 142(7):1441–1458

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

We wish to thank the participating farmers in Zambia. This research was partly supported by Grants-in-Aid for Basic Scientific Research number (A) 17255010 and (C) 18580308 from the Ministry of Education, Culture, Sports, Science and Technology, Japan. The Southern African Centre for Infectious Disease Surveillance is acknowledged for funding CJK to present some of the results detailed in this paper to the 1st One Health Conference in Africa held at the National Institute for Communicable Diseases, Johannesburg, South Africa, July 2011.

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Authors and Affiliations

  1. Department of Veterinary Microbiology and Parasitology, Molecular Biology and Biotechnology Section, Sokoine University of Agriculture, P. O. Box 3019, Chuo kikuu, Morogoro, Tanzania

    C. J. Kasanga

  2. The Avian Zoonoses Center, Faculty of Agriculture, Tottori University, 4-101, Koyama Minami, Tottori, 680-8550, Japan

    T. Yamaguchi

  3. Department of Paraclinical Sciences, School of Veterinary Medicine, University of Zambia, PO Box 32379, Lusaka, Zambia

    H. M. Munang’andu

  4. Laboratory of Veterinary Microbiology, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu, Gifu, 501-1193, Japan

    K. Ohya & H. Fukushi

Authors
  1. C. J. Kasanga
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  2. T. Yamaguchi
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  3. H. M. Munang’andu
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  4. K. Ohya
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  5. H. Fukushi
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Corresponding author

Correspondence to C. J. Kasanga.

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Kasanga, C.J., Yamaguchi, T., Munang’andu, H.M. et al. Genomic sequence of an infectious bursal disease virus isolate from Zambia: classical attenuated segment B reassortment in nature with existing very virulent segment A. Arch Virol 158, 685–689 (2013). https://doi.org/10.1007/s00705-012-1531-4

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  • DOI: https://doi.org/10.1007/s00705-012-1531-4

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