Abstract
We determined the complete nucleotide sequence of an infectious bursal disease (IBD) virus (IBDV) isolate (designated KZC-104) from a confirmed IBD outbreak in Lusaka in 2004. The genome consisted of 3,074 and 2,651 nucleotides in the coding regions of segments A and B, respectively. Alignment of both nucleotide and deduced amino acid sequences and phylogenetic analysis revealed that the genome segment A of KZC-104 was derived from a very virulent (VV) strain, whereas its segment B was derived from a classical attenuated strain. On BLAST search, the full-length segment A and B sequences showed 98 % nucleotide sequence identity to the VV strain D6948 and 99.8 % nucleotide sequence identity to the classical attenuated strain D78. This is a unique IBDV reassortant strain that has emerged in nature, involving segment B of a cell-culture-adapted attenuated vaccine.
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Acknowledgments
We wish to thank the participating farmers in Zambia. This research was partly supported by Grants-in-Aid for Basic Scientific Research number (A) 17255010 and (C) 18580308 from the Ministry of Education, Culture, Sports, Science and Technology, Japan. The Southern African Centre for Infectious Disease Surveillance is acknowledged for funding CJK to present some of the results detailed in this paper to the 1st One Health Conference in Africa held at the National Institute for Communicable Diseases, Johannesburg, South Africa, July 2011.
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Kasanga, C.J., Yamaguchi, T., Munang’andu, H.M. et al. Genomic sequence of an infectious bursal disease virus isolate from Zambia: classical attenuated segment B reassortment in nature with existing very virulent segment A. Arch Virol 158, 685–689 (2013). https://doi.org/10.1007/s00705-012-1531-4
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DOI: https://doi.org/10.1007/s00705-012-1531-4