Abstract
An outbreak of foot-and-mouth disease (FMD) in Egypt affected approximately 40,000 cattle and water buffaloes and killed more than 4,600 animals during February-March 2012. To investigate the etiology of the 2012 outbreak, specimens were collected from six governorates and analyzed using universal primers to amplify the 5′ untranslated region (UTR) by reverse-transcription polymerase chain reaction. Only FMDV-SAT2 was detected, with an overall detection rate of 80.3 %. Complete VP1- and leader-proteinase-coding sequences, obtained from three isolates from three different governorates, were compared with previously reported sequences. Phylogenetic analysis of these sequences indicated that the circulating viruses were homogeneous and were closely related to topotype VII. Importantly, the newly emerged viruses were genetically closely related to strains isolated from Saudi Arabia, Sudan, Eritrea and Cameroon between 2000 and 2010, suggesting the dominant nature of this virus and underscoring the need for worldwide intensive surveillance to minimize its devastating consequences.
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References
Abdul-Hamid NF, Hussein NM, Wadsworth J, Radford AD, Knowles NJ, King DP (2011) Phylogeography of foot-and-mouth disease virus types O and A in Malaysia and surrounding countries. Infect Genet Evol 11:320–328
Alexandersen S, Zhang Z, Donaldson AI, Garland AJ (2003) The pathogenesis and diagnosis of foot-and-mouth disease. J Comp Pathol 129:1–36
Alexandersen S, Mowat N (2005) Foot-and-mouth disease: host range and pathogenesis. Curr Top Microbiol Immunol 288:9–42
Ayelet G, Mahapatra M, Gelaye E, Egziabher BG, Rufeal T, Sahle M, Ferris NP, Wadsworth J, Hutchings GH, Knowles NJ (2009) Genetic characterization of foot-and-mouth disease viruses, Ethiopia, 1981–2007. Emerg Infect Dis 15:1409–1417
Bastos AD, Boshoff CI, Keet DF, Bengis RG, Thomson GR (2000) Natural transmission of foot-and-mouth disease virus between African buffalo (Syncerus caffer) and impala (Aepyceros melampus) in the Kruger National Park, South Africa. Epidemiol Infect 124:591–598
Bastos AD, Haydon DT, Sangare O, Boshoff CI, Edrich JL, Thomson GR (2003) The implications of virus diversity within the SAT 2 serotype for control of foot-and-mouth disease in sub-Saharan Africa. J Gen Virol 84:1595–1606
Bronsvoort BM, Radford AD, Tanya VN, Nfon C, Kitching RP, Morgan KL (2004) Molecular epidemiology of foot-and-mouth disease viruses in the Adamawa province of Cameroon. J Clin Microbiol 42:2186–2196
Chenna R, Sugawara H, Koike T, Lopez R, Gibson TJ, Higgins DG, Thompson JD (2003) Multiple sequence alignment with the Clustal series of programs. Nucleic Acids Res 31:3497–3500
Crowther JR, Rowe CA, Butcher R (1993) Characterization of monoclonal antibodies against a type SAT 2 foot-and-mouth disease virus. Epidemiol Infect 111:391–406
FAO (2012) Major foot-and-mouth outbreak in Egypt threatens the region. http://www.fao.org/news/story/en/item/129919/icode/
Feng Q, Chen X, Ma O, Liu Y, Ding M, Collins RA, Ko LS, Xing J, Lau LT, Yu AC, Chen J (2003) Serotype and VP1 gene sequence of a foot-and-mouth disease virus from Hong Kong (2002). Biochem Biophys Res Commun 302:715–721
Ghoneim NH, Abdel-Karim AK, El-Shehawy L, Abdel-Moein KA (2010) Foot and mouth disease in animals in Sharkia governorate—Egypt. Transbound Emerg Dis 57:19–21
Grubman MJ, Baxt B (2004) Foot-and-mouth disease. Clin Microbiol Rev 17:465–493
Habiela M, Ferris NP, Hutchings GH, Wadsworth J, Reid SM, Madi M, Ebert K, Sumption KJ, Knowles NJ, King DP, Paton DJ (2010) Molecular characterization of foot-and-mouth disease viruses collected from Sudan. Transbound Emerg Dis 57:305–314
Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis for Windows 95/98/NT. Nucleic Acids Symp 41:95–98
ICTV (2012) Viral taxonomy: 2011 Release. http://ictvonline.org/virusTaxonomy.asp?version=2011
Knowles NJ, Samuel AR (2003) Molecular epidemiology of foot-and-mouth disease virus. Virus Res 91:65–80
Knowles NJ, Wadsworth J, Reid SM, Swabey KG, El-Kholy AA, Abd El-Rahman AO, Soliman HM, Ebert K, Ferris NP, Hutchings GH, Statham RJ, King DP, Paton DJ (2007) Foot-and-mouth disease virus serotype A in Egypt. Emerg Infect Dis 13:1593–1596
Kronovetr J, Skern T (2002) Foot-and-mouth disease virus leader proteinase: a papain-like enzyme requiring an acidic environment in the active site. FEBS Lett 528:58–62
Li GJ, Yan WY, Xu QX, Sheng ZT, Zheng ZX (2001) Study on the DNA vaccine against foot-and-mouth disease virus using the heavy chain constant region of swine IgG as the carrier for peptide epitopes. Sheng wu gong cheng xue bao: Chin J Biotechnol 17:322–324
Loth L, Osmani MG, Kalam MA, Chakraborty RK, Wadsworth J, Knowles NJ, Hammond JM, Benigno C (2011) Molecular characterization of foot-and-mouth disease virus: implications for disease control in Bangladesh. Transbound Emerg Dis 58:240–246
OIE (2012) Egypt official report. http://www.oie.int/wahis/public.php?page=event_summary&reportid=9993
Reid SM, Ferris NP, Hutchings GH, Samuel AR, Knowles NJ (2000) Primary diagnosis of foot-and-mouth disease by reverse transcription polymerase chain reaction. J Virol Methods 89:167–176
Rodriguez LL, Grubman MJ (2009) Foot and mouth disease virus vaccines. Vaccine 27(Suppl 4):D90–D94
Rweyemamu M, Roeder P, MacKay D, Sumption K, Brownlie J, Leforban Y (2008) Planning for the progressive control of foot-and-mouth disease worldwide. Transbound Emerg Dis 55:73–87
Rweyemamu M, Roeder P, Mackay D, Sumption K, Brownlie J, Leforban Y, Valarcher JF, Knowles NJ, Saraiva V (2008) Epidemiological patterns of foot-and-mouth disease worldwide. Transbound Emerg Dis 55:57–72
Saeed A, Khan QM, Waheed U, Arshad M, Asif M, Farooq M (2011) RT-PCR evaluation for identification and sequence analysis of foot-and-mouth disease serotype O from 2006 to 2007 in Punjab, Pakistan. Comp Immunol Microbiol Infect Dis 34:95–101
Samuel AR, Knowles NJ (2001) Foot-and-mouth disease type O viruses exhibit genetically and geographically distinct evolutionary lineages (topotypes). J Gen Virol 82:609–621
Sumption K, Rweyemamu M, Wint W (2008) Incidence and distribution of foot-and-mouth disease in Asia, Africa and South America; combining expert opinion, official disease information and livestock populations to assist risk assessment. Transbound Emerg Dis 55:5–13
Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599
Valarcher JF, Leforban Y, Rweyemamu M, Roeder PL, Gerbier G, Mackay DK, Sumption KJ, Paton DJ, Knowles NJ (2008) Incursions of foot-and-mouth disease virus into Europe between 1985 and 2006. Transbound Emerg Dis 55:14–34
van Rensburg H, Haydon D, Joubert F, Bastos A, Heath L, Nel L (2002) Genetic heterogeneity in the foot-and-mouth disease virus Leader and 3C proteinases. Gene 289:19–29
WRLFMD (2012) FMDV genotyping in Egypt. http://www.wrlfmd.org/fmd_genotyping/africa/egy.htm
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Kandeil, A., El-Shesheny, R., Kayali, G. et al. Characterization of the recent outbreak of foot-and-mouth disease virus serotype SAT2 in Egypt. Arch Virol 158, 619–627 (2013). https://doi.org/10.1007/s00705-012-1529-y
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DOI: https://doi.org/10.1007/s00705-012-1529-y