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Soybean chlorotic spot virus, a novel begomovirus infecting soybean in Brazil

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Abstract

A novel soybean-infecting begomovirus from Brazil was identified in Jaíba, in the state of Minas Gerais, and molecularly characterized. By using rolling-circle amplification-based cloning of viral DNAs, three DNA-A variants and a cognate DNA-B were isolated from infected samples. The DNA variants share more than 98 % sequence identity but have less than 89 % identity to other reported begomovirus, the limit for demarcation of new species. In a phylogenetic analysis, both DNA-A and DNA-B clustered with other Brazilian begomoviruses. Infectious cloned DNA-A and DNA-B components induced distinct symptoms in Solanaceae and Fabaceae species by biolistic inoculation. In soybean, the virus induced mild symptoms, i.e., chlorotic spots on the leaves, from which the name soybean chlorotic spot virus (SoCSV) was proposed. The most severe symptoms were displayed by common beans, which exhibited leaf distortion, blistering, interveinal chlorosis, mosaic and golden mosaic. The possibility that SoCSV may become a threat to bean production in Brazil is discussed.

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Acknowledgments

This research was financially supported through the following grants from Brazilian Government Agencies: CNPq grants 559602/2009-0, 573600/2008-2 and 470287/2011-0 (to E.P.B.F.), FAPEMIG grant CBB-APQ-00070-09, and FINEP grant 01.09.0625.00 (to E.P.B.F.). O.J.B.B. and D.C were supported by CAPES graduate fellowships associated with INCT in Plant-Pest Interactions; I.C. was supported by a CNPq graduate fellowship; A.A.S. was supported by a postdoctoral fellowship from CAPES. A.K.I.N. is a CNPq fellow.

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Authors and Affiliations

  1. Departamento de Bioquimica e Biologia Molecular, Universidade Federal de Vicosa, Vicosa, Brazil

    Daniela Coco, Iara P. Calil, Otavio J. B. Brustolini, Anesia A. Santos & Elizabeth P. B. Fontes

  2. National Institute of Science and Technology in Plant-Pest Interactions, Universidade Federal de Vicosa, Bioagro, Vicosa, MG, 36570-000, Brazil

    Daniela Coco, Iara P. Calil, Otavio J. B. Brustolini, Anesia A. Santos, Alice K. Inoue-Nagata & Elizabeth P. B. Fontes

  3. Embrapa Vegetables, Brasília, DF, 70359-970, Brazil

    Alice K. Inoue-Nagata

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  1. Daniela Coco
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  2. Iara P. Calil
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  3. Otavio J. B. Brustolini
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  4. Anesia A. Santos
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  5. Alice K. Inoue-Nagata
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  6. Elizabeth P. B. Fontes
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Corresponding author

Correspondence to Elizabeth P. B. Fontes.

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705_2012_1499_MOESM1_ESM.docx

Supplementary material 1 The common region of DNA-A and DNA-B from SoCSV DNA-A variants and DNA-B. A multiple sequence alignment of common region sequences from the indicated viral components was constructed in Clustal. Gaps (shown as dashes) were allowed to maximize identity. The putative Rep-binding sites (iterons) are underlined and shown in blue, the conserved stem-loop is in bold, and the conserved nonanucleotide is in red. (DOCX 20.4 kb)

705_2012_1499_MOESM2_ESM.tif

Supplementary material 2 Symptoms caused by SoCSV on the newly emerging leaves of plant species from the family Solanaceae. The upper leaves of Capsicum annuum (A and B), Nicotiana rustica (C and D), N. benthamiana (E) and N. Debneyi (F) are shown 21-28 days after the biolistic inoculation of young plants with tandemly repeated SoCSV-DNA-A and DNA-B. (TIFF 1101 kb)

Supplementary material 3 (TIFF 2329 kb)

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Coco, D., Calil, I.P., Brustolini, O.J.B. et al. Soybean chlorotic spot virus, a novel begomovirus infecting soybean in Brazil. Arch Virol 158, 457–462 (2013). https://doi.org/10.1007/s00705-012-1499-0

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  • DOI: https://doi.org/10.1007/s00705-012-1499-0

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