Skip to main content

Advertisement

SpringerLink
Log in

Transcription characteristics of the human cytomegalovirus UL13 gene

  • Brief Report
  • Published:
Archives of Virology Aims and scope Submit manuscript

Abstract

The human cytomegalovirus (HCMV) UL13 gene is located in the unique long (UL) region of its genome. The transcript structure of UL13 gene has not been investigated to date. By using cDNA library screening, northern blot, and rapid amplification of cDNA ends (RACE), the HCMV UL13 gene was demonstrated to be transcribed from the immediate early (IE) to the late (L) phase of infection, and at least one 1602-nt unspliced transcript was identified in the present study from three clinical isolates.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

References

  1. Alderete JP, Jarrahian S, Geballe AP (1999) Translational effects of mutations and polymorphisms in a repressive upstream open reading frame of the human cytomegalovirus UL4 gene. J Virol 73:8330–8337

    PubMed  CAS  Google Scholar 

  2. Britt WJ, Alford CA (eds) (1996) Cytomegalovirus, 3rd edn. Lippincott-Raven, Philadelphia

    Google Scholar 

  3. Chambers J, Angulo A, Amaratunga D, Guo H, Jiang Y, Wan JS, Bittner A, Frueh K, Jackson MR, Peterson PA, Erlander MG, Ghazal P (1999) DNA microarrays of the complex human cytomegalovirus genome: profiling kinetic class with drug sensitivity of viral gene expression. J Virol 73:5757–5766

    PubMed  CAS  Google Scholar 

  4. Chee MS, Bankier AT, Beck S, Bohni R, Brown CM, Cerny R, Horsnell T, Hutchison CA 3rd, Kouzarides T, Martignetti JA (1990) Analysis of the protein-coding content of the sequence of human cytomegalovirus strain AD169. Curr Top Microbiol Immunol 154:125–169

    Article  PubMed  CAS  Google Scholar 

  5. Chomczynski P, Sacchi N (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162:156–159

    Article  PubMed  CAS  Google Scholar 

  6. Davison AJ, Dolan A, Akter P, Addison C, Dargan DJ, Alcendor DJ, McGeoch DJ, Hayward GS (2003) The human cytomegalovirus genome revisited: comparison with the chimpanzee cytomegalovirus genome. J Gen Virol 84:17–28

    Article  PubMed  CAS  Google Scholar 

  7. Dolan A, Cunningham C, Hector RD, Hassan-Walker AF, Lee L, Addison C, Dargan DJ, McGeoch DJ, Gatherer D, Emery VC, Griffiths PD, Sinzger C, McSharry BP, Wilkinson GW, Davison AJ (2004) Genetic content of wild-type human cytomegalovirus. J Gen Virol 85:1301–1312

    Article  PubMed  CAS  Google Scholar 

  8. Dunn W, Trang P, Zhong Q, Yang E, van Belle C, Liu F (2005) Human cytomegalovirus expresses novel microRNAs during productive viral infection. Cell Microbiol 7:1684–1695

    Article  PubMed  CAS  Google Scholar 

  9. Dunn W, Chou C, Li H, Hai R, Patterson D, Stolc V, Zhu H, Liu F (2003) Functional profiling of a human cytomegalovirus genome. Proc Natl Acad Sci USA 25:14223–14228

    Article  Google Scholar 

  10. Fehr AR, Yu D (2010) Human cytomegalovirus gene UL21a encodes a short-lived cytoplasmic protein and facilitates virus replication in fibroblasts. J Virol 84:291–302

    Article  PubMed  CAS  Google Scholar 

  11. Fehr AR, Yu D (2011) Human cytomegalovirus early protein pUL21a promotes efficient viral DNA synthesis and the late accumulation of immediate-early transcripts. J Virol 85:663–674

    Article  PubMed  CAS  Google Scholar 

  12. Grey F, Antoniewicz A, Allen E, Saugstad J, McShea A, Carrington JC, Nelson J (2005) Identification and characterization of human cytomegalovirus- encoded microRNAs. J Virol 79:12095–12099

    Article  PubMed  CAS  Google Scholar 

  13. Isomura H, Stinski MF (2003) The human cytomegalovirus major immediate early enhancer determines the efficiency of immediate-early gene transcription and viral replication in permissive cells at low multiplicity of infection. J Virol 77:3602–3614

    Article  PubMed  CAS  Google Scholar 

  14. Isomura H, Stinski MF, Kudoh A, Daikoku T, Shirata N, Tsurumi T (2005) Two Sp1/Sp3 binding sites in the major immediate-early proximal enhancer of human cytomegalovirus have a significant role in viral replication. J Virol 79:9597–9607

    Article  PubMed  CAS  Google Scholar 

  15. Ma YP, Ruan Q, Ji YH, Wang N, Li ML, Qi Y, He R, Sun ZR, Ren GW (2011) Novel transcripts of human cytomegalovirus clinical strain found by cDNA library screening. Genet Mol Res 10:566–575

    Article  PubMed  CAS  Google Scholar 

  16. Meier JL, Stinski MF (1997) Effect of a modulator deletion on transcription of the human cytomegalovirus major immediate-early genes in infected undifferentiated and differentiated cells. J Virol 71:1246–1255

    PubMed  CAS  Google Scholar 

  17. Meier JL, Pruessner JA (2000) The human cytomegalovirus major immediate early distal enhancer region is required for efficient viral replication and immediate-early gene expression. J Virol 74:1602–1613

    Article  PubMed  CAS  Google Scholar 

  18. Mocarski ES, Shenk T, Pass RF (eds) (2007) Cytomegaloviruses, vol 2, 5th edn. Lippincott Williams & Wilkins, Philadelphia

    Google Scholar 

  19. Pari GS, Anders DG (1993) Eleven loci encoding trans-acting factors are required for transient complementation of human cytomegalovirus oriLyt-dependent DNA replication. J Virol 67:6979–6988

    PubMed  CAS  Google Scholar 

  20. Perng YC, Qian Z, Fehr AR, Xuan B, Yu D (2011) The human cytomegalovirus gene UL79 is required for the accumulation of late viral transcripts. J Virol 85:4841–4852

    Article  PubMed  CAS  Google Scholar 

  21. Wang N, Ma Y, Sun Z, Qi Y, Ji Y, He R, Li M, Ruan Q (2011) Transcriptional features and transcript structure of UL145 in different strains of human cytomegalovirus. J Med Virol 83:2151–2156

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by the National Natural Science Foundation of China (30672248, 30770109, 30700916, 30801254, 30901625, 81171580 and 81171581). The authors declare that they have no conflict of interest.

Author information

Authors and Affiliations

  1. Virus Laboratory, The Affiliated Shengjing Hospital, China Medical University, Shenyang, Liaoning, 110004, China

    Ning Wang, Yanping Ma, Mali Li, Shuang Gao, Lin Wang, Ying Qi, Yaohua Ji, Zhengrong Sun & Qiang Ruan

Authors
  1. Ning Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yanping Ma
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Mali Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Shuang Gao
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Lin Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Ying Qi
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Yaohua Ji
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Zhengrong Sun
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Qiang Ruan
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Qiang Ruan.

Additional information

The first corresponding author is Qiang Ruan.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wang, N., Ma, Y., Li, M. et al. Transcription characteristics of the human cytomegalovirus UL13 gene. Arch Virol 158, 473–477 (2013). https://doi.org/10.1007/s00705-012-1461-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-012-1461-1

Keywords

Search

Navigation