Archives of Virology

, Volume 156, Issue 5, pp 839–854

Feline leukemia virus outbreak in the critically endangered Iberian lynx (Lynx pardinus): high-throughput sequencing of envelope variable region A and experimental transmission

  • C. P. Geret
  • V. Cattori
  • M. L. Meli
  • B. Riond
  • F. Martínez
  • G. López
  • A. Vargas
  • M. A. Simón
  • J. V. López-Bao
  • R. Hofmann-Lehmann
  • H. Lutz
Original Article

DOI: 10.1007/s00705-011-0925-z

Cite this article as:
Geret, C.P., Cattori, V., Meli, M.L. et al. Arch Virol (2011) 156: 839. doi:10.1007/s00705-011-0925-z

Abstract

The Iberian lynx is the most endangered felid species. During winter/spring 2006/7, a feline leukemia virus (FeLV) outbreak of unexpected virulence killed about 2/3 of the infected Iberian lynxes. All FeLV-positive animals were co-infected with feline hemoplasmas. To further characterize the Iberian lynx FeLV strain and evaluate its potential virulence, the FeLV envelope gene variable region A (VRA) mutant spectrum was analyzed using the Roche 454 sequencing technology, and an in vivo transmission study of lynx blood to specified-pathogen-free cats was performed. VRA mutations indicated weak apolipoprotein B mRNA editing enzyme and catalytic polypeptide-like cytidine deaminase (APOBEC) restriction of FeLV replication, and variants characteristic of aggressive FeLV strains, such as FeLV-C or FeLV-A/61C, were not detected. Cats exposed to FeLV/Candidatus Mycoplasma haemominutum-positive lynx blood did not show a particularly severe outcome of infection. The results underscore the special susceptibility of Iberian lynxes to infectious diseases.

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • C. P. Geret
    • 1
  • V. Cattori
    • 1
  • M. L. Meli
    • 1
  • B. Riond
    • 1
  • F. Martínez
    • 2
  • G. López
    • 3
  • A. Vargas
    • 2
  • M. A. Simón
    • 3
  • J. V. López-Bao
    • 4
  • R. Hofmann-Lehmann
    • 1
  • H. Lutz
    • 1
  1. 1.Clinical Laboratory, Vetsuisse FacultyUniversity of ZurichZurichSwitzerland
  2. 2.Centro de Cria en Cautividad de Lince Ibérico El AcebucheEspacio Natural de DoñanaMatalascañasSpain
  3. 3.Egmasa-Consejería de Medio AmbienteCórdobaSpain
  4. 4.Departamento de Biología de la ConservaciónEstación Biológica de Doñana (CSIC)SevilleSpain

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