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Aspergillus europaeus sp. nov., a widely distributed soil-borne species related to A. wentii (section Cremei)

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Abstract

A new species belonging to Aspergillus section Cremei is described, A. europaeus sp. nov. This species is most closely related to A. wentii, A. dimorphicus, and A. chrysellus and can be distinguished by an unique phenotype (colour of sporulation, production of yellow soluble pigment on MEA, shape of vesicle, ornamentation of conidia), specific spectrum of produced exometabolites and internal transcribed spacers (ITS), β-tubulin and calmodulin gene sequence data. In total, 18 isolates were identified which originated from soil (n = 14), cork (n = 2), indoor air (n = 1) and clinical material (n = 1) in the Czech Republic, Romania, Spain, Portugal, France and Tunisia. All strains were first identified by morphological characters as A. wentii or A. tamarii suggesting that A. europaeus is a common species but it has been overlooked and frequently misidentified. The ex-type strain is CCF 4409T = CBS 134393T = IBT 32228T = NRRL 66252T.

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Acknowledgments

This research was supported by the Ministry of Education, Youth and Sports of the Czech Republic (SVV project) and by the project “BIOCEV—Biotechnology and Biomedicine Centre of the Academy of Sciences and Charles University” (CZ.1.05/1.1.00/02.0109) from the European Regional Development Fund. Molecular genetic analyses were supported by the projects of Charles University Grant Agency (GAUK 1130214). The survey in European caves was supported through a grant from the Czech Science Foundation (Grant No. P506-12-1064). We thank M. Chudíčková for her invaluable assistance in laboratory, C. S. Pereira, C. Barreto and P. Lysková for supplying isolates of A. europaeus.

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Authors and Affiliations

  1. Department of Botany, Faculty of Science, Charles University in Prague, Benátská 2, 128 01, Prague 2, Czech Republic

    Vit Hubka, Alena Nováková, František Sklenář & Miroslav Kolařík

  2. Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i, Vídeňská 1083, 142 20, Prague 4, Czech Republic

    Vit Hubka, Alena Nováková, František Sklenář & Miroslav Kolařík

  3. CBS-KNAW Fungal Biodiversity Centre, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands

    Robert A. Samson & Jos Houbraken

  4. Technical University of Denmark, Søltofts Plads, Bldg. 221—DTU Systems Biology, Kgs, 2800, Lyngby, Denmark

    Jens C. Frisvad

  5. Department of Microbiology, Faculty of Science and Informatics, University of Szeged, Szeged, 6726, Hungary

    Janos Varga

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  1. Vit Hubka
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Online Resource 1. Alignment of partial calmodulin gene sequences used for reconstruction of tree on Figure 1.

Online Resource 2. Alignment of partial β-tubulin gene sequences used for reconstruction of tree on Figure 1.

Online Resource 3. Alignment of ITS rDNA region sequences used for reconstruction of tree on Figure 1.

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Hubka, V., Nováková, A., Samson, R.A. et al. Aspergillus europaeus sp. nov., a widely distributed soil-borne species related to A. wentii (section Cremei). Plant Syst Evol 302, 641–650 (2016). https://doi.org/10.1007/s00606-016-1293-7

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