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Phylogenetic relationships of New Zealand Lycopodiaceae

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Abstract

We examine the global relationships of all New Zealand’s Lycopodiaceae species through DNA sequencing of the chloroplast rbcL gene and phylogeny reconstruction. The molecular phylogeny largely agreed with recent taxonomic schemes based on morphology. However, the grouping of Lycopodiella serpentina with Lycopodiella caroliniana, as either Lycopodiella sect. Caroliniana or Pseudolycopodiella was not supported in the phylogeny, indicating that these classification schemes need adjustment. Several New Zealand species showed intraspecific genetic variation within New Zealand and/or compared to conspecific samples from overseas. The chloroplast sequences could not distinguish Lycopodiella diffusa and Lycopodiella lateralis, nor the different morphologies of Phlegmariurus varius characteristic of high and low altitudes.

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References

  • Aagaard SM, Greilhuber J, Zhang XC, Wikstrom N (2009) Occurrence and evolutionary origins of polyploids in the clubmoss genus Diphasiastrum (Lycopodiaceae). Molec Phylogenet Evol 52:746–754. doi:10.1016/j.ympev.2009.05.004

    Article  CAS  PubMed  Google Scholar 

  • Allan HH (1961) Flora of New Zealand, vol 1. Government Printer, Wellington

    Google Scholar 

  • Braekman JC, Nyembo L, Bourdoux P, Kahindo N, Hootle C (1974) Distribution des alcaloides dans le genre Lycopodium. Phytochemistry 13:2519–2528

    Article  CAS  Google Scholar 

  • Brownsey PJ, Smith-Dodsworth JC (2000) New Zealand ferns and allied plants, 2nd edn. David Bateman Ltd, Auckland

    Google Scholar 

  • Brownsey PJ, Given DR, Lovis JD (1985) A revised classification of New Zealand pteridophytes with a synonymic checklist of species. New Zealand J Bot 23:431–489

    Article  Google Scholar 

  • Bruce JG (1976) Development and distribution of mucilage canals in Lycopodium. Amer J Bot 63:481–491

    Article  Google Scholar 

  • Christenhusz MJM, Chase MW (2014) Trends and concepts in fern classification. Ann Bot (Oxford) 113:571–594. doi:10.1093/aob/mct299

    Article  Google Scholar 

  • Christenhusz MJM, Zhang X, Schneider H (2011) A linear sequence of extant families and genera of lycophytes and ferns. Phytotaxa 19:7–54

    Article  Google Scholar 

  • Darriba D, Taboada GL, Doallo R, Posada D (2012) jModelTest 2: more models, new heuristics and parallel computing. Nat Meth 9:772. doi:10.1038/nmeth.2109

    Article  CAS  Google Scholar 

  • Ebihara A, Nitta JH, Ito M (2010) Molecular species identification with rich floristic sampling: barcoding the pteridophyte flora of Japan. PLOS One 5:E15136

    Article  PubMed  PubMed Central  Google Scholar 

  • Field AR, Bostock PD (2013) New and existing combinations in Palaeotropical Phlegmariurus (Lycopodiaceae) and lectotypification of the type species Phlegmariurus phlegmaris (L.) T. Sen and U. Sen. PhytoKeys 20:33–51. doi:10.3897/phytokeys.20.4007

    Article  PubMed  Google Scholar 

  • Field AR, Testo W, Bostock PD, Holtum JAM, Waycott M (2016) Molecular phylogenetics and the morphology of the Lycopodiaceae subfamily Huperzioideae supports three genera: Huperzia, Phlegmariurus and Phylloglossum. Molec Phylogenet Evol 94:635–657. doi:10.1016/j.ympev.2015.09.024

    Article  CAS  PubMed  Google Scholar 

  • Guindon S, Dufayard JF, Lefort V, Anisimova M, Hordijk W, Gascuel O (2010) New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. Syst Biol 59:307–321. doi:10.1093/sysbio/syq010

    Article  CAS  PubMed  Google Scholar 

  • Hasebe M, Omori T, Nakazawa M, Sano T, Kato M, Iwatsuki K (1994) rbcL gene sequences provide evidence for the evolutionary lineages of leptosporangiate ferns. Proc Natl Acad Sci USA 91:5730–5734

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Holloway JE (1919) Studies in the New Zealand species of the genus Lycopodium: part III—the plasticity of species. Trans & Proc Roy Soc New Zealand 51:161–216

    Google Scholar 

  • Holub J (1983) Validation of generic names in Lycopodiaceae: with a description of a new genus Pseudolycopodiella. Folia Geobot Phytotax 18:439–442

    Article  Google Scholar 

  • Holub J (1985) Transfers of Lycopodium species to Huperzia: with a note on generic classification in Huperziaceae. Folia Geobot Phytotaxon 20:67–80

    Article  Google Scholar 

  • Holub J (1991) Some taxonomic changes within Lycopodiales. Folia Geobot Phytotax 26:81–94

    Article  Google Scholar 

  • Huelsenbeck JP, Ronquist FR (2001) MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics 17:754–755

    Article  CAS  PubMed  Google Scholar 

  • Ji S-G, Huo K-K, Wang J, Pan S-L (2008) A molecular phylogenetic study of Huperziaceae based on chloroplast rbcL and psbA-trnH sequences. J Syst Evol 46:2130219. doi:10.3724/SP.J.1002.2008.07036

    Google Scholar 

  • Kenrick P, Crane PR (1997) The origin and early evolution of plants on land. Nature 389:33–39

    Article  CAS  Google Scholar 

  • Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, Valentin F, Wallace IM, Wilm A, Lopez R, Thompson JD, Gibson TJ, Higgins DG (2007) Clustal W and clustal X version 2.0. Bioinformatics 23:2947–2948

    Article  CAS  PubMed  Google Scholar 

  • Øllgaard B (1987) A revised classification of the Lycopodiaceae s. lat. Opera Bot 92:153–178

    Google Scholar 

  • Øllgaard B (2012) New combinations in neotropical Lycopodiaceae. Phytotaxa 57:10–22

    Article  Google Scholar 

  • Perrie LR, Ohlsen DJ, Shepherd LD, Garrett M, Brownsey PJ, Bayly MJ (2010) Tasmanian and Victorian populations of the fern Asplenium hookerianum result from independent dispersals from New Zealand. Austral Syst Bot 23:387–392

    Article  Google Scholar 

  • Pryer KM, Schneider H, Smith AR, Cranfill R, Wolf PG, Hunt JS, Sipes SD (2001) Horsetail and ferns are a monophyletic groups and the closest living relatives to seed plants. Nature 409:618–622

    Article  CAS  PubMed  Google Scholar 

  • Pryer KM, Schneider H, Magallón S (2004) The radiation of ferns, horsetails and seed plants. In: Cracraft J, Donoghue M (eds) Assembling the tree of life. Oxford University Press, New York, pp 138–153

    Google Scholar 

  • Rambaut A, Drummond AJ (2009) Tracer, version 1.6. Available at: http://tree.bio.ed.ac.uk/software/tracer/. Accessed 5 Nov 2015

  • Schuettpelz E, Pryer KM (2007) Fern phylogeny inferred from 400 leptosporangiate species and three plastid genes. Taxon 56:1037–1050. doi:10.2307/25065903

    Article  Google Scholar 

  • Shepherd LD, McLay TGB (2011) Two micro-scale protocols for the isolation of DNA from polysaccharide-rich plant tissue. J Pl Res 124:311–314. doi:10.1007/s10265-010-0379-5

    Article  CAS  Google Scholar 

  • Shepherd LD, Perrie LR (2014) Genetic analyses of herbarium material: is more care required? Taxon 63:972–973. doi:10.12705/635.2

    Article  Google Scholar 

  • Shepherd LD, Perrie LR, Brownsey PJ (2007) Fire and ice: volcanic and glacial impacts on the phylogeography of the New Zealand forest fern Asplenium hookerianum. Molec Ecol 16:4536–4549

    Article  CAS  Google Scholar 

  • Swofford DL (2002) PAUP*. Phylogenetic analysis using parsimony (*and other methods). Version 4. Sinauer Associates, Sunderland, Massachusetts

  • Tryon RM, Tryon AF (1982) Ferns and allied plants with special reference to tropical America. Springer, New York

    Book  Google Scholar 

  • Untergasser A, Cutcutache I, Koressaar T, Ye J, Faircloth BC, Remm M, Rozen SG (2012) Primer3—new capabilities and interfaces. Nucl Acids Res 40:e115

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Wikström N, Kenrick P (1997) Phylogeny of Lycopodiaceae (Lycopsida) and the relationships of Phylloglossum drummondii Kunze based on rbcL sequences. Int J Pl Sci 158:862–871

    Article  Google Scholar 

  • Wikström N, Kenrick P (2000) Relationships of Lycopodium and Lycopodiella based on combined plastid rbcL gene and trnL intron sequence data. Syst Bot 25:495–510

    Article  Google Scholar 

  • Wikström N, Kenrick P (2001) Evolution of Lycopodiaceae (Lycopsida): estimating divergence times from rbcL gene sequences by use of nonparametric rate smoothing. Molec Phylogenet Evol 19:177–186

    Article  PubMed  Google Scholar 

  • Yatsentyuk SP, Valiejo-Roman KM, Samigullin TH, Wilkstrom N, Troitsky AV (2001) Evolution of Lycopodiaceae inferred from spacer sequencing of chloroplast rRNA genes. Russ J Genet 37:1068–1073

    Article  CAS  Google Scholar 

  • Zhang LB, Iwatsuki K (2013) Lycopodiaceae. In: Wu ZY, Raven PH, Hong DY (eds) Flora of China, Vols 2–3 (Pteridophytes). Science Press, Missouri Botanical Garden Press, Beijing, St Louis, pp 13–34

    Google Scholar 

Download references

Acknowledgments

Delaney Burnard acknowledges support from the Te Papa M.Sc. Scholarship in molecular systematics and evolution, and Lara Shepherd was supported by a Royal Society of New Zealand Rutherford Discovery Fellowship. We thank the Department of Conservation, Wellington City Council and Greater Wellington Regional Council for granting permission to collect in reserve land. We thank Pat Brownsey, Peter Beveridge, Matt von Konrat and Louis Thouvenot for assistance with collecting and Pat Brownsey, Benjamin Øllgaard and an anonymous reviewer for comments on the manuscript.

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Authors and Affiliations

  1. School of Biological Sciences, Victoria University of Wellington, PO Box 600, Wellington, New Zealand

    Delaney Burnard, Lara Shepherd & Andrew Munkacsi

  2. Museum of New Zealand Te Papa Tongarewa, PO Box 467, Wellington, New Zealand

    Lara Shepherd & Leon Perrie

Authors
  1. Delaney Burnard
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  2. Lara Shepherd
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  3. Leon Perrie
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  4. Andrew Munkacsi
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Correspondence to Lara Shepherd.

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Handling editor: Jochen Heinrichs.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1

Localities, voucher and GenBank accession numbers for samples. (PDF 116 kb)

Supplementary material 2

Alignment of rbcL sequences. (PDF 195 kb)

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Information on Electronic Supplementary Material

Online Resource 1. Localities, voucher and GenBank accession numbers for samples

Online Resource 2. Alignment of rbcL sequences

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Burnard, D., Shepherd, L., Perrie, L. et al. Phylogenetic relationships of New Zealand Lycopodiaceae. Plant Syst Evol 302, 661–667 (2016). https://doi.org/10.1007/s00606-016-1290-x

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  • DOI: https://doi.org/10.1007/s00606-016-1290-x

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