Plant Systematics and Evolution

, Volume 299, Issue 9, pp 1637–1643

Comparison of SSR and cytochrome P-450 markers for estimating genetic diversity in Picrorhiza kurrooa L.

Original Article

DOI: 10.1007/s00606-013-0820-z

Cite this article as:
Katoch, M., Hussain, M.A. & Ahuja, A. Plant Syst Evol (2013) 299: 1637. doi:10.1007/s00606-013-0820-z


Picrorhiza kurrooa L., a high altitude medicinal plant, is known for its drug content called Kutkin. In the present study, DNA-based molecular marker techniques, viz. simple sequence repeats (SSR) and cytochrome P-450 markers were used to estimate genetic diversity in Picrorhiza kurrooa. Twenty five accessions of Picrorhiza kurrooa, collected from ten different eco-geographical locations were subjected to 22 SSR and eight cytochrome P-450 primer pairs, out of which 13 SSR markers detected mean 5.037 alleles with a mean polymorphic information content (PIC) of 0.7718, whereas eight cytochrome P-450 markers detected mean 5.0 alleles with a mean PIC of 0.7596. Genetic relationship among the accessions was estimated by constructing the dendrograms using SSR and cytochrome P-450 data. There was a clear consistency between SSR and cytochrome P-450 trees in terms of positioning of most Picrorhiza accessions. SSR markers could cluster various Picrorhiza kurrooa accessions based on their geographical locations whereas cytochrome P-450 markers could cluster few accessions as per their geographical locations. The Mantel test between SSR and cytochrome P-450 markers revealed a good fit correlation (r = 0.6405). The dendrogram constructed using the combined data of SSR and cytochrome P-450s depicted two clusters of accessions based on its eco-geographical locations whereas two clusters contained the accessions from mixed eco-geographical locations. Overall, the results of the present study point towards quiet high degree of genetic variation among the accessions of each eco-geographic region.


Picrorhiza kurrooa L.Genetic diversityShort sequence repeats (SSR)

Copyright information

© Springer-Verlag Wien 2013

Authors and Affiliations

  1. 1.Biotechnology DivisionIndian Institute of Integrative Medicine (CSIR)JammuIndia
  2. 2.Biodiversity and Applied Botany DivisionIndian Institute of Integrative Medicine (CSIR)JammuIndia