Abstract
This article addresses the vegetative anatomy (leaves, stems, roots, root tubers and rhizomes) of 13 species of subfamily Orchidoideae (Orchidaceae), belonging to the genera Neottia Guettard, Cephalanthera L.C.M. Richard, Epipactis Zinn, Limodorum Boehmer, Spiranthes L.C.M. Richard, Platanthera L.C.M. Richard, Serapias L., Himantoglossum W.D. Koch and Anacamptis L.C.M. Richard, because anatomical studies have provided very useful criteria for orchid diagnosis. In the study three types of painting methods—Delafield’s hematoxylin and safranin, Alcian blue-periodic acid schiff, and alcoholic phloroglucinol + HCl—were employed, and identification tables were prepared. Anatomical results demonstrated the differences in the leaf anatomy of tuberous and rhizomatous orchids. In the stem anatomy, all the rhizomatous genera were found to be anatomically different, especially in regard to the collateral vascular bundles, the distribution of vascular bundles and xylem properties. In root anatomy, the central cylinder, pith, endodermis and/or pericycle properties are distinctive features in all studied taxa. For root tubers, velamen layering, wall outline mucilage cell patterns in ground tissue and arrangements of vascular arches can be used to label taxa. Regarding the rhizome anatomy of the studied taxa, vascular cylinder results in particular were very significant for the distinction of genera. Finally, we strongly emphasize the importance of this kind of detailed anatomical study to solve identification problems of orchid taxonomy.
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References
Aybeke M (2000) Edirne Çevresindeki Ophrys L. (Orchidaceae) Türleri Üzerinde Karyolojik Araştırmalar. Sist Bot Herb 7(1):187–196
Aybeke M (2002) In vitro pollen germination experiments on granular pollens and polliniums in Orchids. Gazi Univ J Sci 15(1):71–80
Aybeke M, Sezik E, Olgun G (2010) Vegetative anatomy of some Ophrys, Orchis and Dactylorhiza (Orchidaceae) taxa in Trakya region of Turkey. Flora 205(2):73–89
Borsos O (1980) Anatomy of wild orchids in Hungary. I. Tissue structure of lead and floral axis. Act Agr Acad Sci Hung 29:369–389
Burr B, Barthlott W (1991) On a velamen-like tissue in the root cortex of Orchids. Flora 185:313–323
Davies KL, Stpiczynska M (2010) Structure and distribution of floral trichomes in Lycaste and Sudamerlycaste (Orchidaceae: Maxillariinae s.l.). Bot J Linn Soc 164:409–421
Delforge P (2005) Guide des orchidées d’Europe, d’Afrique du Nord et du Proche-Orient. Timber Press, Oregon
Dressler RL (1993) Phylogeny and classification of the orchids family. Dioscorides Press, Portland
Foster AS (1956) Plant idioblasts: remarkable examples of cell specializations. Protoplasma 46:184–193
Gaffney E (1989) Carbohydrates. In: Edna B, Prophet BM, Jacquelyn BA, Leslie H, Sobin ML (eds) Laboratory methods in histotechnology. American Registry of Pathology, Washington, pp 149–170
Holtzmeier MA, Stern WL, Judd WS (1998) Comparative anatomy and systematics of Senghas’s cushion species of Maxillaria (Orchidaceae). Bot J Linn Soc 127:43–82
Jurcak J (2000) Der innere Bau vegetativer Organe einiger europäischer Orchideen: Teil 7 Diskussion und Zusammenfassung. Orchidee 51(2):177–180
Kreutz VJA (2000) Orchidaceae. In: Güner A, Özhatay N, Ekim T, Başer KHC (eds) Flora of Turkey and the East Aegean Islands (supplement 2). Edinburg Univ Press, Edinburgh, pp 274–305
Kurzweil H, Linder HP, Stern WL (1995) Comparative vegetative anatomy and classification of Diseae (Orchidaceae). Bot J Linn Soc 111:411–455
MnManus JFA (1948) Histological and histochemical uses of periodic acid. Stain Technol 23:99
Olatunji OA, Nengim RO (1980) Occurrence and distribution of tracheoidal elements in the Orchidaceae. Bot J Linn Soc 80:357–370
Prete CD, Miceli P (1999) Histoanatomical and taxonomical observations on some Central Mediterranean entities of Orchis Sect. Labellotrilobatae P.Vermeul. Subsections Masculae Newski and Provinciales Newski (Orchidee). Caesiana Quaderno 12:21–44
Pridgeon AM (1981) Absorbing trichomes in the Pleurothallidinae (Orchidaceae). Amer J Bot 69:921–938
Pridgeon AM (1982) Diagnostic anatomical characters in the Pleurothallidinae (Orchidaceae). Amer J Bot 69:921–938
Pridgeon AM (1993) Systematic anatomy of Orchidaceae. Resource or anachronism? In: Proceedings of the 14th world orchid conference, Glasgow. HMSO, Edinburgh, pp 84–91
Pridgeon AM (1994) Systematic leaf anatomy of Caladeniinae (Orchidaceae). Bot J Linn Soc 114:31–48
Pridgeon AM, Chase MW (1995) Subterranean axes in tribe Diurideae (Orchidaceae): morphology, anatomy and systematics significance. Amer J Bot 82:1473–1495
Rasmussen HN (1981) The diversity of stomatal development in Orchidaceae subfamily Orchidoideae. Bot J Linn Soc 82:381–393
Samuel J, Bhat RB (1994) Epidermal structure, organographic distribution and ontogeny of stomata in vegetative and floral organs of Stenoglottis fimbriata (Orchidaceae). S Afr J Bot 60:113–117
Sezik E (1988) Trakya’da yetişen Orchidaceae Türleri. Trakya Florası Sempozyumu Bildiri Özetleri. Trakya University, Edirne, pp 40–44
Stern WL (1997) Vegetative anatomy of subtribe Orchidinae (Orchidaceae). Bot J Linn Soc 124:121–136
Stern WL, Judd WS (2001) Comparative anatomy of Catasetiinae (Orchidaceae). Bot J Linn Soc 136:153–178
Stern WL, Morris MW (1992) Vegetative anatomy of Stanhopea (Orchidaceae) with special reference to pseudo bulb water-storage cells. Lindleyana 7:34–53
Stern WL, Morris MW, Judd WS, Pridgeon AM, Dressler RL (1993) Comparative vegetative anatomy and systematics Of Spiranthoideae (Orchidaceae). Bot J Linn Soc 113:161–197
Vogelmann TC, Nishio JN, Smith WK (1996) Leaves and light capture: light propagation and gradients of carbon fixation within leaves. Tr in Plant Sci 1:65–70
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Aybeke, M. Comparative anatomy of selected rhizomatous and tuberous taxa of subfamilies Orchidoideae and Epidendroideae (Orchidaceae) as an aid to identification. Plant Syst Evol 298, 1643–1658 (2012). https://doi.org/10.1007/s00606-012-0666-9
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DOI: https://doi.org/10.1007/s00606-012-0666-9