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Determination of α2,3-sialylated glycans in human serum using a glassy carbon electrode modified with carboxylated multiwalled carbon nanotubes, a polyamidoamine dendrimer, and a glycan-recognizing lectin from Maackia Amurensis

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Abstract

α2,3-Sialylated glycans (α2,3-sial-Gs) are crucial molecular targets for cancer diagnosis and clinical research. We describe a biosensor for detecting α2,3-sial-Gs that is based on the specific recognition by the Maackia amurensis lectin (MAL). A glassy carbon electrode was modified with carboxy-modified MWCNTs and a polyamidoamine dendrimer (PAMAM). The PAMAM is highly branched and contains many amino groups. MAL was immobilized on the modified electrode to create a sensor capable of detecting α2,3-sial-Gs (at a working voltage of 0.2 V vs. SCE) in the 10 fg⋅mL−1 to 50 ng⋅mL−1 concentration range and with a detection limit as low as 3 fg⋅mL−1 (at an S/N ratio of 3). This approach may pave the way to detection schemes for other proteins and glycans if appropriate affinity binding pairs are available.

A simple biosensor used polyamidoamine dendrimer (PAMAM) grafted p-MWCNTs as platform to detect α2,3-sialylated glycans was developed. It possesses a good linear relationship ranged 10 fg mL−1 to 50 ng mL−1 with a detection limit of 3 fg mL−1.

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Acknowledgments

This study was supported financially by the National Natural Science Foundation of China (81370403, 21205146).

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Authors and Affiliations

  1. Institute of Life Science and School of Public Health, Chongqing Medical University, No.1 Yixueyuan Road, Yuzhong District, Chongqing, 400016, People’s Republic of China

    Yazhen Niu, Junlin He, Yuliang Li, Yilin Zhao, Chunyong Xia, Guolin Yuan, Lei Zhang, Yuchan Zhang & Chao Yu

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  1. Yazhen Niu
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  2. Junlin He
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  3. Yuliang Li
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  4. Yilin Zhao
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  5. Chunyong Xia
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  6. Guolin Yuan
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  7. Lei Zhang
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  8. Yuchan Zhang
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  9. Chao Yu
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Correspondence to Chao Yu.

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The author(s) declare that they have no competing interests

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Junlin He and Yazhen Niu contributed equally to this work.

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Niu, Y., He, J., Li, Y. et al. Determination of α2,3-sialylated glycans in human serum using a glassy carbon electrode modified with carboxylated multiwalled carbon nanotubes, a polyamidoamine dendrimer, and a glycan-recognizing lectin from Maackia Amurensis . Microchim Acta 183, 2337–2344 (2016). https://doi.org/10.1007/s00604-016-1873-9

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  • DOI: https://doi.org/10.1007/s00604-016-1873-9

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