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Colorimetric determination of neomycin using melamine modified gold nanoparticles

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Abstract

The colorimetric assay for neomycin presented here is based on melamine-modified gold nanoparticles (mel-AuNPs) and the finding that hydrogen bonding between melamine and neomycin results in the aggregation of mel-AuNPs. This results in a change in the color of the solution from wine red to blue and in a red-shift of the absorption maximum of the mel-AuNPs. The concentration of neomycin can be determined by spectrophotometry. The ratio of absorptions at 680 nm and 520 nm is linearly related to the logarithm of the concentration of neomycin in the 0.1 to 5.0 nM range and in the 5 to 100 nM range, with regression coefficients of 0.997 and 0.999, respectively. The detection limit (at an S/N ratio of 3) is 30 pM. This is far below the usual safety limit. The method was applied to the detection of trace levels of neomycin in milk samples and gave recoveries between 98 and 105 %.

The presence of neomycin induces the aggregation of melamine-modified gold nanoparticles, this resulting in a color change from red to blue. The concentration of neomycin substrates can be quantified by bare eyes or a UV–Vis spectrometer.

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Acknowledgments

This work was supported by a grant from the Major National Scientific Research Plan of China (973 Program) (Grant No. 2011CB933202) and the National Natural Science Foundation of China (Grant No. 21205132).

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Authors and Affiliations

  1. School of Chemistry and Chemical Engineering, University of Chinese Academy of Sciences, 19A Yuquan Road, Beijing, 100049, China

    Can Xiao, Junfeng Liu, Ankang Yang, Hong Zhao, Yujian He, Xiangjun Li & Zhuobin Yuan

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  1. Can Xiao
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  2. Junfeng Liu
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  3. Ankang Yang
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  4. Hong Zhao
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  5. Yujian He
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  6. Xiangjun Li
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  7. Zhuobin Yuan
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Correspondence to Hong Zhao.

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Xiao, C., Liu, J., Yang, A. et al. Colorimetric determination of neomycin using melamine modified gold nanoparticles. Microchim Acta 182, 1501–1507 (2015). https://doi.org/10.1007/s00604-015-1480-1

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  • DOI: https://doi.org/10.1007/s00604-015-1480-1

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