Microchimica Acta

, 174:97

Biocompatible and label-free amperometric immunosensor for hepatitis B surface antigen using a sensing film composed of poly(allylamine)-branched ferrocene and gold nanoparticles

Original Paper

DOI: 10.1007/s00604-011-0585-4

Cite this article as:
Qiu, JD., Huang, H. & Liang, RP. Microchim Acta (2011) 174: 97. doi:10.1007/s00604-011-0585-4

Abstract

An amperometric immunosensor has been developed for sensitive determination of hepatitis B surface antigen as a model protein. A glassy carbon electrode was modified with an assembly of positively charged poly(allylamine)-branched ferrocene (PAA-Fc) and negatively charged gold nanoparticles (Au NPs). The formation of PAA-Fc effectively avoids the leakage of Fc, retains its electrochemical activity, and enhances the conductivity of the composite. The adsorption of Au NPs onto the PAA-Fc matrix provides sites for the immobilization of the antigen and a favorable micro-environment to maintain its activity. The morphologies and electrochemistry of the sensing film were investigated via scanning electron microscopy, electrochemical impedance spectroscopy, and cyclic voltammetry. Factors influencing the performance of the immunosensor were studied in detail. The concentration of the antigen can be quantitated (by measuring the decrease of the amperometric response resulting from the specific binding between antigen and antibody) in the range between 0.1 and 150 ng mL–1, with a detection limit of 40 pg mL–1 (S/N = 3). The method is economical, efficient, and potentially attractive for clinical immunoassays.

Figure

A novel and sensitive amperometric immunosensor based on the assembly of biocompatible positively charged poly(allylamine)-branched ferrocene and negatively charged Au nanoparticles onto a glassy carbon electrode has been developed for sensitive determination of hepatitis B surface antigen as a model protein.

Keywords

Amperometric immunosensor Poly(allylamine) Ferrocene Gold nanoparticles Hepatitis B surface antigen 

Supplementary material

604_2011_585_MOESM1_ESM.doc (96 kb)
ESM 1(DOC 95 kb)

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  1. 1.Department of Chemistry and Institute for Advanced StudyNanchang UniversityNanchangPeople’s Republic of China

Personalised recommendations