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Electrochemical study of the interaction between dsDNA and copper(II) using carbon paste and hanging mercury drop electrodes

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Abstract

The interaction of copper(II) with double-stranded (ds) calf thymus DNA was studied in solution as well as at the electrode surface by means of differential pulse stripping voltammetry and alternating current voltammetry, using carbon paste electrode and hanging mercury drop electrode, respectively. As a result of the interaction of Cu(II) with dsDNA, the characteristic peak of dsDNA, due to the oxidation of guanine, increased while the oxidation peak of adenine decreased, probably due to the formation of a purine base residue-Cu(II) complex (dsDNA-Cu(II) complex). The interaction of copper(II) with DNA is shown to be time-dependent.

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Acknowledgement

Z. Stanic wish to thank the Ministry of Science of the Republic of Serbia, for financial support.

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Authors and Affiliations

  1. Laboratory of Analytical Chemistry, Department of Chemistry, Aristotle University, Thessaloniki, 54124, Greece

    Stella Girousi

  2. Department of Chemistry, Faculty of Sciences, University of Kragujevac, R. Domanović 12, P.O. Box 60, Kragujevac, 34000, Serbia

    Zorka Stanić

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  1. Zorka Stanić
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  2. Stella Girousi
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Correspondence to Stella Girousi.

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Stanić, Z., Girousi, S. Electrochemical study of the interaction between dsDNA and copper(II) using carbon paste and hanging mercury drop electrodes. Microchim Acta 164, 479–485 (2009). https://doi.org/10.1007/s00604-008-0083-5

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  • DOI: https://doi.org/10.1007/s00604-008-0083-5

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