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Clinical importance of a transcription reverse-transcription concerted (TRC) diagnosis using peritoneal lavage fluids obtained pre- and post-lymphadenectomy from gastric cancer patients

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Abstract

Purpose

Our previous study demonstrated that a transcription reverse-transcription concerted (TRC) diagnosis using peritoneal lavage fluids at laparotomy was a potential prognostic factor for gastric cancer patients; however, the clinical importance of a TRC diagnosis from peritoneal lavage fluids after lymphadenectomy remains unclear.

Methods

TRC amplification targeting CEA mRNA was utilized to detect free cancer cells before and after lymphadenectomy during gastric cancer resection. Of 120 patients who underwent curative gastrectomy for cT2-T4 gastric cancer, 38 whose peritoneal lavage samples were collected pre- and post-lymphadenectomy were eligible for analysis.

Results

Six (16.0 %) patients had CEA positivity before lymphadenectomy (pre-CEA), whereas nine (23.7 %) had CEA positivity after lymphadenectomy (post-CEA). Post-CEA was significantly correlated with higher pathological N-stage. Moreover, the positive post-CEA group had significantly worse relapse-free survival than the negative post-CEA group (p = 0.001). Cox multivariate analysis revealed that post-CEA positivity was a significant predictor of poor relapse-free survival (p = 0.012). It was also a predictor of distant metastasis, but not specific for peritoneal recurrence.

Conclusions

These findings demonstrated that post-CEA positivity in a TRC diagnosis was a potential predictor of disease recurrence, but not a specific predictor of peritoneal recurrence.

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Abbreviations

TRC:

Transcription reverse-transcription concerted reaction

CEA:

Carcinoembryonic antigen

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Authors and Affiliations

  1. Department of Surgery, Osaka Medical Center for Cancer and Cardiovascular Disease, 1-3-3 Nakamichi, Higashinari-ku, Osaka City, 537-8511, Osaka, Japan

    Keijiro Sugimura, Yoshiyuki Fujiwara, Takeshi Omori, Masaaki Motoori, Norikatsu Miyoshi, Hirofumi Akita, Kunihito Gotoh, Shogo Kobayashi, Hidenori Takahashi, Shingo Noura, Masayuki Ohue, Masato Sakon & Masahiko Yano

  2. Department of Clinical Laboratory, Osaka Medical Center for Cancer and Cardiovascular Disease, 1-3-3 Nakamichi, Higashinari-ku, Osaka City, 537-8511, Osaka, Japan

    Takashi Yamamoto

Authors
  1. Keijiro Sugimura
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  2. Yoshiyuki Fujiwara
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  3. Takeshi Omori
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  4. Masaaki Motoori
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  5. Norikatsu Miyoshi
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  6. Hirofumi Akita
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  7. Kunihito Gotoh
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  8. Shogo Kobayashi
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  9. Hidenori Takahashi
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  10. Shingo Noura
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  11. Masayuki Ohue
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  12. Takashi Yamamoto
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  13. Masato Sakon
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  14. Masahiko Yano
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Corresponding author

Correspondence to Yoshiyuki Fujiwara.

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Sugimura, K., Fujiwara, Y., Omori, T. et al. Clinical importance of a transcription reverse-transcription concerted (TRC) diagnosis using peritoneal lavage fluids obtained pre- and post-lymphadenectomy from gastric cancer patients. Surg Today 46, 654–660 (2016). https://doi.org/10.1007/s00595-015-1235-y

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  • DOI: https://doi.org/10.1007/s00595-015-1235-y

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