Surgery Today

, Volume 35, Issue 5, pp 396–403

Quantitative Gene Expression Analysis by cDNA Microarray During Liver Regeneration After Partial Hepatectomy in Rats

  • Hong-Shiee Lai
  • Yun Chen
  • Wen-Hsi Lin
  • Chiung-Nien Chen
  • Hsiu-Chuan Wu
  • Chee-Jen Chang
  • Po-Huang Lee
  • King-Jen Chang
  • Wei-Jao Chen
Article

DOI: 10.1007/s00595-004-2962-7

Cite this article as:
Lai, HS., Chen, Y., Lin, WH. et al. Surg Today (2005) 35: 396. doi:10.1007/s00595-004-2962-7

Abstract

Purpose

It is speculated that genetic regulation plays an important role during liver regeneration. We conducted this study to analyze quantitative gene expression during liver regeneration after partial hepatectomy.

Methods

Sixty male Wistar rats were randomly assigned into ten groups of six. One group of rats was killed preoperatively and the other nine groups were killed 2, 4, 6, 12, 24, 48, and 72 h, and 5 and 7 days after 70% partial hepatectomy, respectively. The remnant livers were isolated for mRNA extraction. A mass survey of gene expression by cDNA microarray carrying 6144 polymerase chain reaction-amplified cDNA fragments was prepared by an arraying machine. The microarray images were scanned, digitized, and analyzed using a flatbed scanner.

Results

The variations in gene expression were classified into 72 different patterns including a pattern with a single peak, 2, 4, 6, 12, and 72 h, and 5 and 7 days after partial hepatectomy. The other patterns included double peaks, enhancing trend, diminished trend, protruding curve, excavated curve, and various mixed types. Each category of gene expression pattern contained 40–218 different proto-oncogenes.

Conclusion

The quantitative gene expression profiles have important implications, warranting further investigation of the genetic mechanisms involved in the process of liver regeneration.

Key words

Gene expressioncDNA microarrayLiver regenerationPartial hepatectomy

Copyright information

© Springer-Verlag Tokyo 2005

Authors and Affiliations

  • Hong-Shiee Lai
    • 1
  • Yun Chen
    • 1
  • Wen-Hsi Lin
    • 1
  • Chiung-Nien Chen
    • 1
  • Hsiu-Chuan Wu
    • 1
  • Chee-Jen Chang
    • 2
  • Po-Huang Lee
    • 1
  • King-Jen Chang
    • 1
  • Wei-Jao Chen
    • 1
  1. 1.Departments of SurgeryNational Taiwan University Hospital and National Taiwan University College of MedicineTaipeiTaiwan
  2. 2.Departments of Medical ResearchNational Taiwan University Hospital and National Taiwan University College of MedicineTaipeiTaiwan