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Injection of a polymerized hyaluronic acid/collagen hydrogel matrix in an in vivo porcine disc degeneration model

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Abstract

Introduction

Disc degeneration and re-herniation after nucleotomy procedures are common problems. Simultaneous application of hyaluronic acid (HA)-based matrix has been proposed to limit disc degeneration. This, however, is hampered by loss of the substituted matrix out of the disc. Hence, in situ polymerization of the injected matrix with ultraviolet light (UVL) directly used after injection may be useful. Therefore, this study evaluates a new HA/collagen hydrogel matrix with in situ polymerization after implantation in an established porcine nucleotomy model.

Materials and methods

12 mature minipigs were used. A total of 60 lumbar discs were analyzed. 36 discs underwent partial nucleotomy with a 16G biopsy needle. Of those, 24 discs received matrix (porcine nucleus pulposus collagenous scaffold component and chemically modified HA) which was in situ polymerized using UVL immediately after transplantation. 12 nucleotomized discs and 24 non-nucleotomized discs served as controls. After 24 weeks, animals were killed. X-rays, MRIs, histology, and gene expression analysis were done.

Results

Disc height was reduced equally after sole nucleotomy and nucleotomy with HA treatment and in MRIs signal intensity decreased. For both nucleotomy groups, the nucleus histo-degeneration score showed a significant increase compared to controls. In histology, HA treatment resulted in more scarring and inflammation in the annulus. Gene expression of catabolic MMPs was up-regulated, whereas IFN-gamma, IL-6, and IL-1b were unchanged.

Conclusion

Although nucleotomy and administration of the implant material did not cause generalized inflammation of the disc, localized annular damage with annulus inflammation and scarring resulted in detrimental degenerative disc changes. As a result, therapeutic strategies should strongly focus on the prevention of annular damage or techniques for annular repair to remain disc integrity.

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Acknowledgments

We thank Bernhard Pieta for helping with animal acquisition and surgical assistance.

Conflict of interest

None.

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Authors and Affiliations

  1. Division of Experimental Orthopaedics, Department of Orthopedics, Trauma Surgery and Paraplegiology, Heidelberg University Hospital, Schlierbacher Landstrasse 200a, 69118, Heidelberg, Germany

    G. W. Omlor, H. Lorenz & W. Richter

  2. Department of Pathology, Academic Hospital Munich-Bogenhausen, 81925, Munich, Germany

    A. G. Nerlich

  3. Institute of Medical Biometry and Informatics, University of Heidelberg, 69118, Heidelberg, Germany

    T. Bruckner

  4. Department of Orthopaedic Surgery, HELIOS Rosmann Hospital Breisach, 79206, Breisach, Germany

    M. Pfeiffer

  5. Department of Trauma and Orthopaedic Surgery, BG Trauma Center Ludwigshafen, University of Heidelberg, 67071, Ludwigshafen, Germany

    T. Gühring

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  1. G. W. Omlor
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  2. A. G. Nerlich
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  3. H. Lorenz
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  4. T. Bruckner
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  5. W. Richter
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  6. M. Pfeiffer
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  7. T. Gühring
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Correspondence to G. W. Omlor.

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Omlor, G.W., Nerlich, A.G., Lorenz, H. et al. Injection of a polymerized hyaluronic acid/collagen hydrogel matrix in an in vivo porcine disc degeneration model. Eur Spine J 21, 1700–1708 (2012). https://doi.org/10.1007/s00586-012-2291-2

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  • DOI: https://doi.org/10.1007/s00586-012-2291-2

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