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Validation, reliability, and complications of a tethering scoliosis model in the rabbit

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Abstract

This study was conducted to refine a small animal model of scoliosis, and to quantify the deformities throughout its growth period. Subcutaneous scapula-to-contralateral pelvis tethering surgery was selected due to its minimally invasive nature and potential applicability for a large animal model. The procedure was performed in 7-week-old New Zealand white rabbits. Group A animals (n=9) underwent the tethering procedure with a suture that spontaneously released. Group B animals (n=17) had the identical procedure with a robust tether and pelvic fixation, which was maintained for 2 months during growth. All animals developed immediate post-operative scoliosis with a Cobb angle of 23° (range, 6–39°) in group A and 59° (range, 24–90°) in group B animals. During the 2 month post-tethering, group A animals lost their tether and scoliosis resolved, whereas all animals in group B maintained their tether until scheduled release at which time the mean scoliosis was 62°. Immediately after tether release, group B scoliosis decreased to a mean 53°. Over the following 4 months of adolescent growth, the scoliosis decreased to a mean of 43° at skeletal maturity; the decrease usually occurred in animals with less than 45° curves at tether release. Radiographs revealed apical vertebral wedging (mean 19°) in all group B animals. Sagittal spinal alignment was also assessed, and for group B animals, the scoliotic segment developed mild to moderate kyphosis (mean 28°) and torsional deformity, but the kyphosis resolved by 4 months after tether-release. Complications specific to this technique included a high rate of transient scapulothoracic dissociation and cases of cor pulmonale. In conclusion, this tethering technique in immature rabbits consistently produced scoliosis with vertebral wedging when the tether was intact through the first 2 months of the protocol. The transient exaggeration of kyphosis suggests that the production of scoliosis is not necessarily dependent on lordosis in this model. Because this technique does not violate thoracic or spinal tissues, it may be useful in the investigation of secondary physiologic effects of mechanically-induced scoliosis, and may be scalable to larger animal species.

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References

  1. Barrios C, Arrotegui JI (1992) Experimental kyphoscoliosis induced in rats by selective brain stem damage. Int Orthop 16:146–151

    Article  PubMed  Google Scholar 

  2. Barrios C, Tunon M, De Salis J, et al (1987) Scoliosis induced by medullary damage: an experimental study in rabbits. Spine 12:433–439

    PubMed  Google Scholar 

  3. Beuerlein M, Wang X, Moreau M, et al (2001) Development of scoliosis following pinealectomy in young chickens is not the result of an artifact of the surgical procedure. Microsc Res Tech 53:81–86

    Article  PubMed  Google Scholar 

  4. Binette MA, Stokes IAF, Aronsson DD, et al (1996) Mechanical modulation of intervertebral disc growth: implications for scoliosis progression. Spine 21:1162–1167

    Article  PubMed  Google Scholar 

  5. Borodic GE (1991) Chemomodulation of curvature of the juvenile spine. US Patent 5,053,005, October 1

  6. Braun JT, Akyuz E (2005) Prediction of curve progression in a goat scoliosis model. J Spinal Disord Tech 18:272–276

    PubMed  Google Scholar 

  7. Braun JT, Ogilvie JW, Akyuz E, et al (2003) Experimental scoliosis in an immature goat model: a method that creates idiopathic-type deformity with minimal violation of the spinal elements along the curve. Spine 28:2198–2203

    Article  PubMed  Google Scholar 

  8. Coillard C, Rhalmi S, Rivard CH (1999) Experimental scoliosis in the minipig: study of vertebral deformations. Ann Chir 53:773–780

    PubMed  Google Scholar 

  9. Court C, Colliou OK, Chin JR, et al (2001) The effect of static in vivo bending on the murine intervertebral disc. Spine 26:239–245

    Google Scholar 

  10. Dickson RA (1987) Idiopathic scoliosis: foundation for physiological treatment. Ann R Coll Surg Engl 69:89–96

    PubMed  Google Scholar 

  11. Geiger B, Steenbock H, Parsons H (1933) Lathyrism in the rat. J Nutr 6:427

    Google Scholar 

  12. Hakkarainen S (1981) Experimental scoliosis: production of structural scoliosis by immobilization of young rabbits in a scoliotic position. Acta Orthop Scand 52:1–57

    PubMed  Google Scholar 

  13. Inoh H, Kawakami N, Matsuyama Y, et al (2001) Correlation between the age of pinealectomy and the development of scoliosis in chickens. Spine 26:1014–1021

    Article  PubMed  Google Scholar 

  14. Kitamura S, Ohara S, Suwa T, Nakagawa K (1965) Studies of vitamin requirements of rainbow trout, Salmo Gairdneri-I. on the ascorbic acid. J Jpn Soc Fish 31:818

    Google Scholar 

  15. Lalic JJ, Angevine DM (1970) Dysostosis in adult rats after prolonged B-aminopropyonitrile feeding. Arch Path 90:22

    PubMed  Google Scholar 

  16. Lawton JO, Dickson RA (1986) The experimental basis of idiopathic scoliosis. Clin Orthop 11:9–17

    Google Scholar 

  17. Lim D, Lovel TR (1978) Pathology of the vitamin C deficiency syndrome in channel catfish (Ictalurus punetatus). J Nutr 108:1137

    PubMed  Google Scholar 

  18. Machida M, Murai I, Miyashita Y, et al (1999) Pathogenesis of idiopathic scoliosis. Experimental study in rats. Spine 24:1985–1989

    Article  PubMed  Google Scholar 

  19. Masoud I, Shapiro F, Kent R, et al (1986) A longitudinal study of the growth of the New Zealand white rabbit: cumulative and biweekly incremental growth rates for body length, body weight, femoral length, and tibial length. J Orthop Res 4:221–231

    Article  PubMed  Google Scholar 

  20. Mente PL, Stokes IAF, Spence H, et al (1997) Progression of vertebral wedging in an asymmetrically loaded rat tail model. Spine 22:1292–1296

    Article  PubMed  Google Scholar 

  21. Newton PO, Gollogly S, Faro F, Marks M (2004) Sagittal hypokyphosis varies with the location of the apex of the coronal curve: is there an explanation that suggests an etiology in idiopathic scoliosis. In: 39th annual meeting of the scoliosis research society, Buenos Aires, 6–9 September 2004

  22. Pincott J, Davies JS, Taffs LF (1984) Scoliosis caused by section of dorsal spinal nerve roots. J Bone Joint Surg 66B:27–29

    Google Scholar 

  23. Robin GC (1995) Scoliosis induced by rib resection in chickens. J Spine Disord 8:179–185

    Google Scholar 

  24. Sarwark JF, Dabney KW, Salzman SK, et al (1988) Experimental scoliosis in the rat. I. Methodology, anatomic features, and neurologic characterization. Spine 13:466–471

    PubMed  Google Scholar 

  25. Smith R, Dickson RA (1987) Experimental structural scoliosis. J Bone Joint Surg 69B:576–581

    Google Scholar 

  26. Stokes IA, Aronsson DD (2001) Disc and vertebral wedging in patients with progressive scoliosis. J Spinal Disord 14:317–322

    Article  PubMed  Google Scholar 

  27. Stokes IA, Mente PL, Iatridis JC, et al (2002) Enlargement of growth plate chondrocytes modulated by sustained mechanical loading. J Bone Joint Surg 84A:1842–1848

    Google Scholar 

  28. Thomas S, Dave PK (1985) Experimental scoliosis in monkeys. Acta Orthop Scand 56:43–46

    PubMed  Google Scholar 

  29. Thometz JG, Liu XC, Lyon R (2000) Three-dimensional rotations of the thoracic spine after distraction with and without rib resection: a kinematic evaluation of the apical vertebra in rabbits with induced scoliosis. J Spinal Disord 13:108–112

    Article  PubMed  Google Scholar 

  30. Tsuang YH, Yang RS, Chen PQ, et al (1992) Experimental structural scoliosis in rabbits. J Formos Med Assoc 91:886–890

    PubMed  Google Scholar 

  31. Wang X, Moreau M, Raso J, et al (1998) Changes in serum melatonin levels in response to pinealectomy in the chicken and its correlation with development of scoliosis. Spine 23:2377–2381

    Article  PubMed  Google Scholar 

  32. Whittle MW, Evans ML (1979) Instrument for measuring the Cobb angle in scoliosis. Lancet 1:414

    Article  PubMed  Google Scholar 

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Acknowledgement

The authors thank Inion, Ltd. for providing the financial support required to complete this study.

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Authors and Affiliations

  1. Midwest Spine Institute, 1950 Curve Crest Blvd, Stillwater, MN, 55082, USA

    Glenn R. Buttermann

  2. Orthopedic Surgery, University of Minnesota, Minneapolis, MN, USA

    Patricia M. Kallemeier, Glenn R. Buttermann, David J. Polga & Kirkham B. Wood

  3. Orthopedic Biomechanics Laboratory, Minneapolis Medical Research Foundation, Minneapolis, MN, USA

    Glenn R. Buttermann, Brian P. Beaubien, Xinqian Chen, William D. Lew & Kirkham B. Wood

Authors
  1. Patricia M. Kallemeier
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  2. Glenn R. Buttermann
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  3. Brian P. Beaubien
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  4. Xinqian Chen
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  5. David J. Polga
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  6. William D. Lew
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  7. Kirkham B. Wood
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Correspondence to Glenn R. Buttermann.

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Kallemeier, P.M., Buttermann, G.R., Beaubien, B.P. et al. Validation, reliability, and complications of a tethering scoliosis model in the rabbit. Eur Spine J 15, 449–456 (2006). https://doi.org/10.1007/s00586-005-1032-1

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  • DOI: https://doi.org/10.1007/s00586-005-1032-1

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