Abstract
Current research indicates that intermittent fasting (IF) alters drug metabolism. The objective of this study was to examine the effect of short-term repeated fasting on some anesthetic parameters of ketamine and xylazine, two anesthetics routinely used in rodents. We divided 50 male mice equally into a fasting and a control group. The fasting group was fasted for 4 continuous days every 2 weeks, and this cycle was repeated four times. During the fasting periods, the fasting mice had access to water only, whereas the control group had free access to both water and food. The mice were allowed a week to recover, then each mouse was anesthetized with a ketamine and xylazine (100 mg/kg and 5 mg/kg) injection, and different parameters of anesthesia were recorded and analyzed. Statistical analysis showed significant differences in all parameters (P < 0.05), indicating that short-term repeated fasting may alter the quality of anesthesia.
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Acknowledgments
We are grateful to the perfect technical assistance of O. Saffar-Benis. We are indebted to Dr. Ghazvinian and Mr. Taghizade for providing the facility and equipments in the Laboratory Animal Research Center for Semnan University (LARC).
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This study was funded by the authors.
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The authors declare that they have no competing interests.
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All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. All procedures were performed under the guidelines for the Care and Use of Laboratory Animals adopted by the Ethics Committee of the School of Veterinary Medicine of Semnan University.
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Shahroozian, E., Davoudi, M., Hayati, F. et al. Effects of short-term repeated fasting on anesthesia: an experimental animal study. Comp Clin Pathol 26, 905–909 (2017). https://doi.org/10.1007/s00580-017-2463-x
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DOI: https://doi.org/10.1007/s00580-017-2463-x