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Bsm1 Gene polymorphism of the vitamin D receptor in breast cancer patients: influence of obesity and relevant drugs

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Abstract

Breast cancer (BC) is a common cancer among women, especially in developing countries. Circulating serum 25-hydroxyvitamin D [25(OH)D] is the key factor that regulates tissue synthesis of the active form 1,25-dihydroxyvitamin D [1,25(OH)2D]. 1,25(OH)2D has anticarcinogenic properties against breast cancer via binding to vitamin D receptors (VDRs). One of the common VDRs gene polymorphism which is located at the 3′ end of VDR gene is the Bsm-I gene polymorphism (rs1544410). Certain VDRs polymorphisms are associated with obesity and show relation to breast cancer risk. The current study was conducted on 60 breast cancer females and 30 healthy control subjects of matched age. Total serum 25(OH) vitamin D was measured using enzyme-linked immunosorbent assay (ELISA) technique. Vitamin D receptor VDR–Bsm1 gene polymorphism was assessed by PCR/RFLP method. Levels of 25(OH) vitamin D were significantly decreased in total, overweight, and obese BC patients in comparison to control group (p = 0.002, <0.001, <0.001, respectively). Regarding Bsm-1 polymorphism, no significant differences were observed between BC patients and control subjects with no risk to develop BC. Bsm-1 gene polymorphism is not associated with breast cancer risk and this result is not affected by increased BMI. A protective effect is observed for [25(OH)D] and ionized calcium levels against BC development.

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Authors and Affiliations

  1. Department of Clinical Pathology, Student hospital, Mansoura University, Mansoura, Egypt

    Noha Mohammed Ali Haikal

  2. Department of Clinical Pathology, Faculty of Medicine, Mansoura University, Mansoura, Egypt

    Mona Abo-Bakr El-Hussiny & Ekbal Mohammed Abo Hashem

  3. Department of Surgical Oncology, Faculty of Medicine, Mansoura University, Mansoura, Egypt

    Omar Farouk

Authors
  1. Noha Mohammed Ali Haikal
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  2. Mona Abo-Bakr El-Hussiny
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  3. Omar Farouk
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  4. Ekbal Mohammed Abo Hashem
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Corresponding author

Correspondence to Mona Abo-Bakr El-Hussiny.

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An informed consent was obtained from all individual participants included in the study, and approval of the local ethics committee of Mansoura University was also obtained with reference cod MS/404.

This work was performed by self financial aid.

Conflict of interest

The authors declare that they have no conflict of interest.

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Haikal, N.M.A., El-Hussiny, M.AB., Farouk, O. et al. Bsm1 Gene polymorphism of the vitamin D receptor in breast cancer patients: influence of obesity and relevant drugs. Comp Clin Pathol 26, 127–134 (2017). https://doi.org/10.1007/s00580-016-2354-6

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  • DOI: https://doi.org/10.1007/s00580-016-2354-6

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