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Hematological and histopathological assessment of pacu (Piaractus mesopotamicus) after treatment of pathogens with veterinary medicinal products

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Abstract

Fish species are impacted by multiple pathogens, and they are exposed to the chemicals used to treat these diseases at several stages during the aquaculture production cycle. This study performed hematological and histopathological evaluations of pacu (Piaractus mesopotamicus) that had been naturally infected with Aeromonas sp., Streptococcus sp., Ichthyophthirius multifiliis, Trichodina heterodentata, and Anacanthorus penilabiatus and treated with enrofloxacin and toltrazuril or with florfenicol and thiamethoxam. After 7 days of treatment from nine fishes were collected blood, via caudal puncture, and samples of the gills, liver, and kidneys. Following toltrazuril and enrofloxacin treatment, fish exhibited leukocytosis with lymphocytosis. With thiamethoxam and florfenicol treatment, the fish showed an increase in hematocrit, hemoglobin level, mean corpuscular volume, and mean corpuscular hemoglobin concentration and a decrease in red blood cells. The infected control fish presented aneurysms and a disruption of the secondary lamellae, which can cause death. The drugs used in this study stimulated the immune systems in the fish or caused electrolyte imbalances, which were temporary.

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Acknowledgments

To FAPESP, scholarship no. 09888-6/2010 and supporting grant no. 08453-9/2011.

State of Sao Paulo Research Foundation process nos. 2008/51900-3 and 2011/08453-9.

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Authors and Affiliations

  1. Centro de Aquicultura da UNESP, Via de Acesso Prof. Dr. Paulo Donato Castellane, s/ no, Zona Rural, Jaboticabal, São Paulo, 14884-900, Brazil

    S P Carraschi, T Florêncio, N F Ignácio & C V Ikefuti

  2. Núcleo de Estudos e Pesquisas Ambientais em Matologia (NEPEAM) da UNESP, Jaboticabal, São Paulo, Brazil

    S P Carraschi, T Florêncio, N F Ignácio & C Cruz

  3. Fundação Educacional de Barretos (UNIFEB), Barretos, SP, Brazil

    C Cruz

  4. Instituto de Pesca, São Paulo, SP, Brazil

    M J T Ranzani-Paiva

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  1. S P Carraschi
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  2. T Florêncio
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  3. N F Ignácio
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  4. C V Ikefuti
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  5. C Cruz
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  6. M J T Ranzani-Paiva
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Correspondence to S P Carraschi.

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All procedures involving animals were in accordance with the ethical standards of the institution at which the studies were conducted and were approved by the University’s Institutional Animal Care and Use Committee under approval number 017335/10.

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Carraschi, S.P., Florêncio, T., Ignácio, N.F. et al. Hematological and histopathological assessment of pacu (Piaractus mesopotamicus) after treatment of pathogens with veterinary medicinal products. Comp Clin Pathol 26, 105–114 (2017). https://doi.org/10.1007/s00580-016-2351-9

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