Abstract
Metabolic syndrome (MetS) is characterized by clustering of clinical, physiological, biochemical, and metabolic factors that are associated with an increased risk of cardiovascular disease (CVD) and type 2 diabetes mellitus. Immune response to heat shock protein 27 (Hsp27) has been suggested to be implicated in atherogenesis. We aimed to investigate the association between serum anti-Hsp27 antibody concentrations and type 2 diabetes in patients with MetS. This was a cross-sectional observational study on groups of MetS and healthy subjects. The population sample was derived from MASHAD STUDY, a national cross-sectional study conducted by the Ministry of Health and Medical Education in 2004. Pregnant and breastfeeding women and patients who had cardiovascular disease, myocardial infarction, stroke, or systemic disease were excluded from the 9600 subjects of the MASHAD STUDY population. A total of 933 subjects including 477 women and 456 men were classified as having MetS, diabetes mellitus, or neither. Data including age, gender, and smoking habit collected using a questionnaire. MetS was diagnosed based on the International Diabetes Federation (IDF) definition. The serum anti-HSP27 antibody titers were measured by ELISA. There was no difference in serum anti-HSP27 concentrations between subjects with and without MetS, or diabetes mellitus, nor was there a significant difference in anti-HSP27 levels between men and women. There was no significant difference in anti-HSP27 antibody between diabetic MetS patients, normal population, non-diabetic MetS, and diabetic non-MetS patients (p value >0.05). Serum anti-Hsp27 antibody concentrations did not differ between individuals with or without MetS or diabetes mellitus.
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References
Abulafia-Lapid R, Elias D, Raz I, Keren-Zur Y, Atlan H, Cohen I (1999) T cell proliferative responses of type 1 diabetes patients and healthy individuals to human hsp60 and its peptides. J Autoimmun 12(2):121–129
Alberti KGM, Zimmet P, Shaw J (2005) The metabolic syndrome—a new worldwide definition. Lancet 366(9491):1059–1062
Azimi-Nezhad M, Ghayour-Mobarhan M, Safarian M, Esmailee H, Parizadeh SMR, Rajabi-Moghadam M, Gholami A, Oladi MR, Ferns GA (2009) Anthropometric indices of obesity and the prediction of cardiovascular risk factors in an Iranian population. Sci World J 9:424–430
Burut DF, Borai A, Livingstone C, Ferns G (2010) Serum heat shock protein 27 antigen and antibody levels appear to be related to the macrovascular complications associated with insulin resistance: a pilot study. Cell Stress Chaperones 15(4):379–386. doi:10.1007/s12192-009-0152-7
Cameron AJ, Shaw JE, Zimmet PZ (2004) The metabolic syndrome: prevalence in worldwide populations. Endocrinol Metab Clin N Am 33(2):351–375
Dreher D, Vargas JR, Hochstrasser DF, Junod AF (1995) Effects of oxidative stress and Ca2+ agonists on molecular chaperones in human umbilical vein endothelial cells. Electrophoresis 16:1205–1214. doi:10.1002/elps.11501601201
Giardino I, Edelstein D, Brownlee M (1996) BCL-2 expression or antioxidants prevent hyperglycemia-induced formation of intracellular advanced glycation endproducts in bovine endothelial cells. J Clin Invest 97:1422–1428. doi:10.1172/JCI118563
Hooper PL, Hooper PL (2009) Inflammation, heat shock proteins, and type 2 diabetes. Cell Stress Chaperones 14(2):113–115
Kaur J (2014) A comprehensive review on metabolic syndrome. Cardiol Res Pract 2014:943162. doi:10.1155/2014/943162
Ota S, Horigome K, Ishii T, Nakai M, Hayashi K, Kawamura T, Kishino A, Taiji M, Kimura T (2009) Metformin suppresses glucose-6-phosphatase expression by a complex I inhibition and AMPK activation-independent mechanism. Biochem Biophys Res Commun 388(2):311–316
Pauli D, Tonka CH, Tissieres A, Arrigo AP (1990) Tissue-specific expression of the heat shock protein HSP27 during Drosophila melanogaster development. J Cell Biol 111:817–828
Riganò R, Profumo E, Buttari B, Tagliani A, Petrone L, D’Amati G, Ippoliti F, Capoano R, Fumagalli L, Salvati B, Businaro R (2007) Heat shock proteins and autoimmunity in patients with carotid atherosclerosis. Ann N Y Acad Sci 1107:1–10
Sahebkar A, Pourghadamyari H, Moohebati M, Parizadeh SM, Falsoleiman H, Dehghani M, Fazlinezhad A, Akhlaghi S, Tavallaie S, Paydar R, Ghayour-Mobarhan M, Ferns GA (2011) A cross-sectional study of the association between heat shock protein 27 antibody titers, pro-oxidant-antioxidant balance and metabolic syndrome in patients with angiographically defined coronary artery disease. Clin Biochem 44:1390–1395
Sims TJ, Lernmark A, Mancl LA, Schifferle RE, Page RC, Persson GR (2002) Serum IgG to heat shock proteins and Porphyromonas gingivalis antigens in diabetic patients with periodontitis. J Clin Periodontol 29(6):551–562
Song Y, Shi J, Wu Y, Han C, Zou J, Shi Y, Liu Z (2014) Metformin ameliorates insulin resistance in L6 rat skeletal muscle cells through upregulation of SIRT3. Chin Med J 127(8):1523–1529
Young RA, Elliott TJ (1989) Stress proteins, infection, and immune surveillance. Cell 59(1):5–8
Acknowledgments
We thank all the patients and their family members who volunteered to participate in this study. Also, the authors would like to thank Mashhad University of Medical Sciences (MUMS) for financial support. The results presented in this work are part of Mr. Mehrdad Kargari’s thesis in MUMS.
This work was supported by Mashhad University of Medical Science (MUMS), Iran.
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This research is financially supported by the Mashhad University of Medical Sciences.
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This research approved by the Mashhad University of Medical Sciences Ethics Committee.
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Informed consent was obtained from all individual participants included in the study.
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The authors declare that they have no conflict of interest.
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Mehrdad Kargari and Seyed Mohammad Reza Parizadeh contributed equally to this work.
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Kargari, M., Parizadeh, S.M.R., Karimian, M.S. et al. Serum anti-HSP27 antibody titers in patients with metabolic syndrome, with or without diabetes mellitus. Comp Clin Pathol 25, 895–901 (2016). https://doi.org/10.1007/s00580-016-2279-0
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DOI: https://doi.org/10.1007/s00580-016-2279-0