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Removal of spermatogonial stem cells (SSCs) from in vitro culture modulates testosterone synthesis in bovine

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Abstract

Niche cells, encompassing spermatogonial stem cells (SSCs) and controlling their development are believed to have a reciprocal communication with SSCs. The present study was conducted to evaluate the impact of the elimination of SSCs on testosterone production and genes contributing to testosterone synthesis. Following isolation, bovine testicular cells were cultured for 12 days on extracellular matrix-coated plates. In the germ cell-removed group, the SSCs were removed from the in vitro culture using differential plating; however, in the control group, no intervention in the culture was performed. Testosterone concentration and the gene expression of luteinizing hormone (LH) receptor (LHR), CYP17A1, and steroidogenic acute regulatory protein (StAR), were assessed on days 6 and 12. The concentration of testosterone was lower in the germ cell-removed than control group (P < 0.05). Moreover, quantitative real-time PCR revealed lower gene expression of LHR, CYP17A1, and StAR in the germ cell-removed group as compared with the control group (P < 0.05). In conclusion, the present study showed that elimination of SSCs from in vitro culture could diminish testosterone production via downregulation of factors contributing to testosterone synthesis. Additionally, this study provided evidence for the reciprocal relationship between SSCs and their niche cells.

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Acknowledgments

The present study was supported by the Theriogenology Association, Faculty of Veterinary Medicine, University of Tehran. The authors would like to thank Mr. S. Pour Beiranvand for his kind assistance in the present study.

Authors’ contribution

VA performed the experiments and statistical analyses and wrote the manuscript. VA, PT, and MM participated in the study design and revised the manuscript. RY participated in the implementation of experiments. All authors read and approved the final manuscript.

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Correspondence to Parviz Tajik.

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Animal Ethics Committee at University of Tehran approved the present study in terms of animal welfare and ethics.

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

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The authors declare that they have no conflict of interest.

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Akbarinejad, V., Tajik, P., Movahedin, M. et al. Removal of spermatogonial stem cells (SSCs) from in vitro culture modulates testosterone synthesis in bovine. Comp Clin Pathol 25, 785–790 (2016). https://doi.org/10.1007/s00580-016-2264-7

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  • DOI: https://doi.org/10.1007/s00580-016-2264-7

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