Skip to main content

Advertisement

SpringerLink
Log in

Sub-acute toxicity of lantadenes isolated from Lantana camara leaves in guinea pig animal model

  • Original Article
  • Published:
Comparative Clinical Pathology Aims and scope Submit manuscript

Abstract

The sub-acute toxicity of lantadenes isolated from Lantana camara leaves was studied in guinea pig laboratory animal model. A total of 20 guinea pigs of either sex were divided into five groups. Group I served as control, while groups II, III, IV, and V were orally administered lantadenes at the dose rate of 100, 50, 25, and 12.5 mg/kg body weight (bw), respectively for 28 days. The levels of aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, acid phosphatase, total bilirubin, blood urea nitrogen, and creatinine were estimated in serum. The effect of lantadenes on lipid peroxidation, superoxide dismutase, reduced glutathione, and catalase was monitored in liver homogenate. The lantadenes at the dose rate of 25 mg/kg bw in animals of group IV did not result in mortality, produced gross and histopathological changes characteristic of sub-acute toxicity in liver and kidneys, and resulted in the highest levels of serum liver marker enzymes. Elevated lipid peroxidation levels and decreased levels of superoxide dismutase and catalase in group IV indicated free-radical-induced damage by lantadenes. It was concluded that lantadenes produced a dose-dependent toxicity and the dose level of 25 mg/kg bw was the sub-acute toxic dose which induced significant fibrosis and necrosis in liver of guinea pigs. To the best of our knowledge, this is the first report on the study of sub-acute toxicity of lantadenes in guinea pig animal model which mimic the actual livestock lantana toxicity cases in field conditions.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9
Fig. 10
Fig. 11
Fig. 12
Fig. 13
Fig. 14
Fig. 15
Fig. 16
Fig. 17
Fig. 18

Similar content being viewed by others

References

  • Aebi HE (1983) In: Bergmeyer HU (ed) Methods of enzymatic analysis, vol III, 3rd edn. Verlag Chemie, Weinheim, pp 273–285

    Google Scholar 

  • Bahadure RS, Bijwal DL, Sadekar RD, Mode SG (1992) Efficacy of Trefoil in prevention of experimental lantana toxicity in calves. India J Vet Med 12:49–51

    Google Scholar 

  • Bergmeyer HU (1974) U.V: method of acid phosphatase assay. In: Methods of enzymatic analysis. Weinheim. Deer field beach, Florida. Vol 3rd, pp. 223

  • Davies KJA, Pryor WA (2005) The evolution of free radical biology & medicine: a 20-year history. Free Radic Biol Med 39:1263–1290

    Article  CAS  PubMed  Google Scholar 

  • Dawra RK, Sharma OP, Makkar HPS (1984) Evidence for a novel antioxidant in bovine seminal plasma. Biochem Int 8:655–659

    CAS  PubMed  Google Scholar 

  • Day MD, Wiley CJ, Playford J, Zalucki MP (2003) Lantana: current management status and future prospects. Australian Center for International Agricultural Research, Canberra

    Google Scholar 

  • Dudnik LB, Tsyupko AN, Khrenov AV, Alessenko AV (2001) Effect of bilirubin on lipid peroxidation, sphingomyelinase activity and apoptosis induced by sphingosine and UV irradiation. Biochemistry Mosc 66:1019–1027

    Article  CAS  PubMed  Google Scholar 

  • Ghisalberti EL (2000) Lantana camara. L. (Verbenaceae). Fitoterapia 71:467–486

    Article  CAS  PubMed  Google Scholar 

  • Gopinath C, Ford EJH (1969) The effect of Lantana camara on liver of sheep. J Path 99:75–85

    Article  CAS  PubMed  Google Scholar 

  • Joly AB (1993) Introducaoa taxonomia vegetal. Sao Paulo: Companhia Editora Nacional

  • Kaushal AK (1994) Clinico-biochemical and therapeutic study on induced lantana toxicosis in bovines. M.V. Sc thesis submitted to H.P.K.V, Palampur, (H.P) India

  • Lowry OH, Roxbrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265–274

    CAS  PubMed  Google Scholar 

  • Luna LG (1968) Manual of histologic staining methods of the armed forces institute of pathology, 3rd edn. McGraw-Hill, New York

    Google Scholar 

  • McKenzie RA (1991) Bentonite as therapy for Lantana camara poisoning of cattle. Aus Vet J 68:146–148

    Article  CAS  Google Scholar 

  • Munir AA (1996) A revision taxonomic of Lantana camara L. and L. montevidensis Briq. (Verbenaceae) in Australia. J Adelaide Bot Gard 17:1–27

    Google Scholar 

  • Munya SJN, Njenga NJ, Karitu TP, Kimora TP, Kiptoon JE, Buoro IBJ (1998) A note of clinical, pathological finding serum enzyme activity in sheep, goat and friesian calves in acute lantana camara poisoning. Bull Anim Health Prod Afr 38:275–279

    Google Scholar 

  • Nishikimi M, Appajirao N, Yaki K (1972) The occurrence of superoxide anion in the reaction of reduced Phenazine methosulphate and molecular oxygen. Biochem Biophys Res Commun 46:849–854

    Article  CAS  PubMed  Google Scholar 

  • Nyblom H, Bjornsson E, Simren E, Aldenborg F, Almer S, Olsson R (2006) The AST/ALT ratio as an indicator of cirrhosis in patients with PBC. Liv Int 26:840–845

    Article  CAS  Google Scholar 

  • Parimoo HA, Sharma R, Patil RD, Sharma OP, Kumar P, Kumar N (2014) Hepatoprotective effect of Ginkgo biloba leaf extract on lantadenes-induced hepatotoxicity in guinea pigs. Toxicon 81:1–12

    Article  CAS  PubMed  Google Scholar 

  • Pass MA (1991) Poisoning of livestock by lantana plants. In: Keeler R, Anthony T (eds) Handbook of natural toxins, toxicology of plant and fungal compounds, 6th edn. Marcel Dekker, Inc, New York, pp 297–311

    Google Scholar 

  • Pass MA, Seawright AA, Heath TJ, Gemmell RT (1978) Lantana poisoning: a cholestatic disease of cattle and sheep. In: Keeler RF, Van Kampen KR, James LF (eds) Effects of poisonous plants on livestock. Academic, New York

    Google Scholar 

  • Pass MA, McSweeney CS, Reynoldson JA (1981) Absorption of the toxins of Lantana camara L. from the digestive system of sheep. J Appl Toxicol 1:38–42

    Article  CAS  PubMed  Google Scholar 

  • Reiter RJ, Tan D, Osuna C, Gitto E (2000) Action of melatonin in the reduction of oxidative stress. J Biomed Sci 7:444–458

    Article  CAS  PubMed  Google Scholar 

  • Sedlak J, Lindsay RH (1968) Estimation of total, protein-bond and non protein sulfhydryl groups in tissue with Ellman’s reagent. Analy Biochem 25:192–205

    Article  CAS  Google Scholar 

  • Sharma OP, Makkar HPS (1981) Lantana-the foremost livestock killer in Kangra district of Himachal pradesh. Livest Adv 6:29–31

    Google Scholar 

  • Sharma S, Sharma OP (1999) Effect of lantadene A-induced toxicity on enzymes associated with cholestasis in blood plasma of guinea pigs. Med Sci Res 27:157–158

    CAS  Google Scholar 

  • Sharma OP, Dawra RK, Makkar HPS (1982) Effect of Lantana camara toxicity on lipid peroxidation in guinea pig tissues. Res Commun Chem Pathol Pharmac 38:153–156

    CAS  Google Scholar 

  • Sharma OP, Makkar HPS, Dawra RK (1983) Effect of lantana toxicity on lysosomal and cytosol enzymes in guinea pig. Toxicol Lett 16:41–45

    Article  CAS  PubMed  Google Scholar 

  • Sharma OP, Makkar HPS, Dawra RK (1988) A review of the noxious plant Lantana camara. Toxicon 26:975–987

    Article  CAS  PubMed  Google Scholar 

  • Sharma OP, Dawra RK, Pattabhi V (1991a) Molecular structure, polymorphism and toxicity of lantadene A, the pentacyclic triterpenoid from the hepatotoxic plant Lantana camara. J Biochem Toxicol 6:57–63

    Article  CAS  PubMed  Google Scholar 

  • Sharma OP, Vaid J, Sharma PD (1991b) Comparison of lantadenes content and toxicity of different taxa of the lantana plant. J Chem Ecol 17:2283–2291

    Article  CAS  PubMed  Google Scholar 

  • Sharma OP, Vaid J, Pattabhi V, Bhutani KK (1992) Biological action of lantadene C, a new hepatotoxicant from Lantana camara var. aculeala. J Mol Biochem Toxicol 7:73–79

    Article  CAS  Google Scholar 

  • Sharma OP, Sharma S, Pattabhi V, Mahato SB, Sharma PD (2007) A Review of the hepatotoxic plant Lantana camara. Crit Rev Toxicol 37:313–352

    Article  CAS  PubMed  Google Scholar 

  • Snedecor GW, Cochran WG (1968) Statistical methods, 6th edn. Oxford and IBH Publishing Company, New Delhi

    Google Scholar 

  • Spies JJ (1984) A Cytotaxonomic study of Lantana Camara (Verbenaceae) from South Africa. S Afr J Bot 3:231–250

    Google Scholar 

  • Stirton CH (1977) Some thoughts on the polyploidy complex Lantana camara L. (verbenecae). Proc. 2nd., National Weeds Conference Balkania pp. 321–340

  • Trauner M, Meier PJ, Boyer JL (1998) Mechanisms of disease: molecular pathogenesis of cholestasis. N Eng J Medi 339:1217–1227

    Article  CAS  Google Scholar 

  • Wang BJ, Liu CT, Tseng CY, Wu CP, Yu ZR (2004) Hepatoprotective and antioxidant effects of Bupleurumkaoiliu (choaetchang) extract and its fractions fractionated using supercritical CO2 on CCl4-induced liver damage. Food Chem Toxicol 42:609–617

    Article  CAS  PubMed  Google Scholar 

  • Yao P, Li K, Jin Y, Song F, Zhou S, Son X (2006) Oxidative damage after chronic ethanol intake in rat tissues: prophylaxisis of Ginkgo biloba extract. Food Chem 99:305–314

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors gratefully acknowledge Dr. O. P. Sharma, Ex-Principal Scientist and Scientist Incharge, ICAR-IVRI Regional Station, Palampur, for critical suggestions and help during the study. We are thankful to the Director, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, and Indian Council of Agricultural Research, New Delhi, India, for providing financial support.

Conflict of interest

All the authors do not have any conflict of interest.

Author information

Authors and Affiliations

  1. Disease Investigation Laboratory, ICAR-Indian Veterinary Research Institute, Regional Station, Palampur, 176061, Himachal Pradesh, India

    Haroon Ashraf Parimoo & Rinku Sharma

  2. Department of Veterinary Pathology, DGCN College of Veterinary and Animal Sciences, CSK HPKV, Palampur, 176062, Himachal Pradesh, India

    Rajendra Damu Patil

  3. CSIR-Institute of Himalayan Bioresource Technology, Palampur, 176061, Himachal Pradesh, India

    Vikram Patial

Authors
  1. Haroon Ashraf Parimoo
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Rinku Sharma
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Rajendra Damu Patil
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Vikram Patial
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Rinku Sharma.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Parimoo, H.A., Sharma, R., Patil, R.D. et al. Sub-acute toxicity of lantadenes isolated from Lantana camara leaves in guinea pig animal model. Comp Clin Pathol 24, 1541–1552 (2015). https://doi.org/10.1007/s00580-015-2114-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00580-015-2114-z

Keywords

Search

Navigation