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Topical co-administration of flaxseed and pistachio ointment promoted wound healing; evidence for histopathological features

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Abstract

This study was conducted to compare the traditional prescriptions of flaxseed and Pistacia atlantica oil in wound healing in rabbit. Eighty white New Zealand rabbits randomly allocated to five experimental groups (n = 16). Two full-thickness skin excisional wounds were created on the thoracolumbar region on each side of the dorsal midline in each rabbit. Group 1 was considered as control. Group 2 rabbits received nitrofurazone ointment (0.2 %, w/w). Group 3 animals were treated with P. atlantica hull (2 %) ointment. In group 4, rabbits received flaxseed ointment (2 %), and group 5 was treated with combination of flaxseed (2 %) + P. atlantica hull (2 %) ointment. Animals were anesthetized, and the skin of specimens were taken in 3, 7, 14, and 21 days postoperative. Tissue samples were dissected out and quantity of cellular infiltration (polymorphonuclear and mononuclear cells, new vessels, and fibroblast formation) and angiogenesis were estimated. Also, the wound-healing area (mm2) was evaluated in 4, 8, 12, 16, and 20 days postoperative. According to the result, flaxseed and pistachio oil ointment significantly decreased polymorphonuclear and mononuclear cell distribution on day 3 postoperative (P < 0.05). Also, co-administration of flaxseed + pistachio oil significantly improved new vessel formation compared to other groups (P < 0.05). Furthermore, flaxseed and pistachio improved subsequent fibroblast distribution in injured rabbit (P < 0.05). Moreover, rabbits, which received the combination of flaxseed + pistachio, had better wound healing compared to control group (P < 0.05). Our data suggested that co-administration of the pistachio and flaxseed promoted the healing process by shortening inflammation phase, elevating cellularity, and promoting collagen synthesis.

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References

  • Abad ANA, Nouri MHK, Tavakkoli F (2011) Effect of salvia officinalis hydroalcoholic extract on vincristine-induced neuropathy in mice. Chinese J Natur Med 9(5):354–358

    Google Scholar 

  • Asadpour R, Azari M, Hejazi M, Tayefi H, Zaboli N (2013) Protective effects of garlic aqueous extract (Allium sativum), vitamin E, and N-acetylcysteine on reproductive quality of male rats exposed to lead. Vet Res Forum 4(4):251–257

    PubMed Central  PubMed  Google Scholar 

  • Boelsma E, Hendriks HFJ, Roza L (2001) Nutritional skin care: health effects of micronutrients and fatty acids. Am J Clin Nutr 73:853–864

    CAS  PubMed  Google Scholar 

  • Chen W, Liou S, Tzeng T, Lee S, Liu I (2012) Wound repair and anti-inflammatory potential of Lonicera japonica in excision wound-induced rats. Compl Alter Med 12(226):2–9

    Google Scholar 

  • Datta HS, Mitra SK, Patwardhan B (2011) Wound healing activity of topical application forms based on ayurveda. Evid Based Complement Alternat Med. doi:10.1093/ecam/nep015

    Google Scholar 

  • De Souza Franco E, De Aquino CMF, De Medeiros PL, Evencio LB, Da SilvaG’oes AJ, De Souza M, Bernadete M (2012) Effect of a semisolid formulation of Linum usitatissimum L. (Linseed) oil on the repair of skin wounds. Evidence-Based Complement Altern Med. doi:10.1155/2012/270752

    Google Scholar 

  • Derakhshanfar A, Oloumi MM, Mirzaie M (2010) Study on the effect of Peganum harmala extract on experimental skin wound healing in rat: pathological and biomechanical findings. Comp Clin Pathol 19:169–172

    Article  Google Scholar 

  • Estevão LRM, de Souza MF, Baratella-Evêncio L, Simões RS, de Barros MEG, Arantes RME, Rachid MA, Evêncio-Neto J (2013) Effects of aroeira (Schinus terebinthifoliu Raddi) oil on cutaneous wound healing in rats. Acta Cir Bras 28(3):202–209

    Article  PubMed  Google Scholar 

  • Farahpour MR, Habibi M (2012) Evaluation of the wound healing activity of an ethanolic extract of Ceylon cinnamon in mice. Vet Med 57(1):53–57

    Google Scholar 

  • Farahpour MR, Taghikhani H, Habibi M, Zandieh MA (2011) Wound healing activity of flaxseed Linum usitatissimum L. in rats. Afr J Pharm Pharmacol 5(21):2386–2389

    CAS  Google Scholar 

  • Francis AA (2012). The effects of dietary flaxseed on atherosclerotic plaque regression. Master of Science Thesis in Physiology. University of Manitoba

  • Giner-Larza EM, Ma’n˜ez S, Recio MC, Giner RM, Prieto JM, Cerda’-Nicol’as M, R’ıos JL (2001) Oleanonic acid, a 3-oxotriterpene from Pistacia, inhibits leukotriene synthesis and has anti-inflammatory activity. Eur J Pharmacol 428:137–143

    Article  CAS  PubMed  Google Scholar 

  • Koch AE, Polverini PJ, Kunkel SL, Harlow LA, DiPietro LA, Elner VM, Elner SG, Strieter RM (1992) Interleukin-8 as a macrophage-derived mediator of angiogenesis. Science 258:1789–1800

    Article  Google Scholar 

  • Loutrari H, Magkouta S, Pyriochou A et al (2006) Mastic oil from Pistacia lentiscus var. chia inhibits growth and survival of human K562 leukemia cells and attenuates angiogenesis. Nutr Cancer 55(1):86–93

    Article  CAS  PubMed  Google Scholar 

  • Magiatis P, Melliou E, Skaltsounis AL, Chinou IB, Mitaku S (1999) Chemical composition and antimicrobial activity of the essential oils of Pistacia lentiscus var chia. Planta Med 65(08):749–752

    Article  CAS  PubMed  Google Scholar 

  • Martin JM, Zenilman JM, Lazarus GSL (2010) Molecular microbiology: new dimensions for cutaneous biology and wound healing. J Investig Dermatol 130:38–48

    Article  CAS  PubMed  Google Scholar 

  • McDaniel JC, Massey K, Nicolaou A (2011) Fish oil supplementation alters levels of lipid mediators of inflammation in microenvironment of acute human wounds. Wound Repair Regen 19(2):189–200

    Article  PubMed Central  PubMed  Google Scholar 

  • Naeini AT, Oryan A, Dehghani S, Nikahval B (2008) Experimental cutaneous wound healing in rabbits: using continuous microamperage low-voltage electrical stimulation. Comp Clin Pathol 17:203–210

    Article  Google Scholar 

  • Oryan A, Naeini AT, Nikahval B, Gorjian E (2010) Effect of aqueous extract of Aloe vera on experimental cutaneous wound healing in rat. Vet Arhiv 80(4):509–522

    Google Scholar 

  • Purohit SK, Solanki R, Soni MK, Mathur V (2012) Experimental evaluation of aloe vera leaves pulp as topical medicament on wound healing. Intern J Pharmacol Res 2(3):110–112

    Article  Google Scholar 

  • Rahimi R, Baghaei A, Baeeri M, Amin G, Shams-Ardekani MR, Khanavi M, Abdollahi M (2013) Promising effect of Magliasa, a traditional Iranian formula, on experimental colitis on the basis of biochemical and cellular findings. World J Gastroenterol 19(12):1901–1911

    Article  PubMed Central  PubMed  Google Scholar 

  • Sanz M, Terencio M, Paya M (1992) Isolation and hypotensive activity of a polymeric procyanidin fraction from Pistacia lentiscus L. Pharmazie 47(6):466–467

    CAS  PubMed  Google Scholar 

  • Sharifi R, Rastegar H, Kamalinejad M, Dehpour AR, Tavangar SM, Paknejad M, Natanzi MM, Ghannadian N, Akbari M, Pasalar P (2012) Effect of topical application of silymarin (Silybum marianum) on excision wound healing in albino rats. Acta Medica Iranica 50(9):583–588

    CAS  PubMed  Google Scholar 

  • Shtayeh AM, Abu Ghdeib SI (1999) Antifungal activity of plant extracts against dermatophytes. Mycoses 42(11–12):665–672

    Article  Google Scholar 

  • Subramanian S, Sathish Kumar D, Arulselvan P (2006) Wound healing potential of Aloe vera leaf gel studied in experimental rabbits. Asian J Biochem 1:178–185

    Article  Google Scholar 

  • Thomas A, Wynn K, Luke B (2010) Macrophages: master regulators of inflammation and fibrosis. Semin Liver Dis 30(3):245–257

    Article  Google Scholar 

  • Tohidi M, Khayami M, Nejati V, Meftahizade H (2011) Evaluation of antibacterial activity and wound healing of Pistacia atlantica and Pistacia khinjuk. J Medic Plant Res 5(17):4310–4314

    Google Scholar 

  • TrivellatoGrassi L, Malheiros A, Meyre-Silva C, da Silva BZ, Monguilhott ED, Fröde TS et al (2013) From popular use to pharmacological validation: a study of the anti-inflammatory, anti-nociceptive and healing effects of Chenopodium ambrosioides extract. J Ethnopharmacol 145(1):127–138

    Article  CAS  PubMed  Google Scholar 

  • Tumen I, Suntar I, Keles H, Akkol EK (2012) A therapeutic approach for wound healing by using essential oils of Cupressus and Juniperus species growing in turkey. Evid Based Complement Alternat Med. doi:10.1155/2012/728281

    PubMed Central  PubMed  Google Scholar 

  • Walker HL, Mason JAD (1968) A standard animal burn. J Trauma Inj Infect Critical Care 8(6):1049–1051

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors wish to thank laboratory section of the Aydin Dilmaghanian (Ph.D) for laboratory tests and analyses.

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Authors declare no conflict of interest.

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Authors and Affiliations

  1. Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, 5715944867, Iran

    Mohammad Reza Farahpour

  2. Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, Iran

    Salar Fathollahpour

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  1. Mohammad Reza Farahpour
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  2. Salar Fathollahpour
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Correspondence to Mohammad Reza Farahpour.

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Farahpour, M.R., Fathollahpour, S. Topical co-administration of flaxseed and pistachio ointment promoted wound healing; evidence for histopathological features. Comp Clin Pathol 24, 1455–1461 (2015). https://doi.org/10.1007/s00580-015-2097-9

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  • DOI: https://doi.org/10.1007/s00580-015-2097-9

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