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Changes in serum levels of purines in an experimental infection by Leptospira interrogans serovar Icterohaemorrhagiae

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Abstract

The aim of this study was to measure the levels of purines in serum of rats experimentally infected with Leptospira interrogans serovar Icterohaemorrhagiae. For this purpose, 24 adult male Wistar rats were divided into two groups: A, not infected (subgroups A1 and A2), and B, infected (subgroups B1 and B2), which were intraperitoneally inoculated (day 0) with 2 × 108 leptospires/animal. Blood samples were collected on days 15 (A1 and B1) and 30 PI (A2 and B2) for analysis of purine levels in serum (adenosine triphosphate (ATP), adenosine diphosphate (ADP), adenosine monophosphate (AMP), adenosine, inosine, hypoxanthine, xanthine, and uric acid). The levels of ATP, AMP, adenosine, and xanthine increased (P < 0.015), while a reduction (P < 0.015) in the concentration of ADP and inosine occurred at day 15 PI. On day 30 PI, ATP, ADP, adenosine, and uric acid significantly increased (P < 0.015), while xanthine level was reduced (P < 0.015). The correlation among the infection and purine levels lead us to suggest the purinergic system as a compensatory mechanism during the leptospirosis infection, mainly due to the actions of ATP, which usually is involved in a pro-inflammatory response, followed by a protective action of ADP and adenosine on the clotting events, along with a protective cellular damage caused by the infection.

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Authors and Affiliations

  1. Departamento de Pequenos Animais, Hospital Veterinário, Universidade Federal de Santa Maria (UFSM), Sala 103, CEP 97105-900, Santa Maria, RS, Brazil

    Alexandre A. Tonin & Sonia T. A. Lopes

  2. Departamento de Zootecnia, Universidade do Estado de Santa Catarina, Rua Beloni Trombeta Zanin, 680-E. Bairro Santo Antônio, CEP 89815-630, Chapecó, SC, Brazil

    Aleksandro S. Da Silva

  3. Departamento de Microbiologia e Parasitologia, UFSM, Santa Maria, RS, Brazil

    Jeandre A. Jaques, Viviane C. G. Souza, Daniela B. R. Leal & Claudio A. M. Leal

  4. Universidade do Extremo Sul Catarinense, UNESC, Bloco S Subsolo, Criciúma, SC, Brazil

    Paula T. Tonin

  5. Departamento de Patologia, Hospital Veterinário, Universitário, UFSM, prédio 97B, Av. Roraima, no. 1000, Camobi, Brazil

    Camila Tochetto

  6. Departamento de Ciências Morfológicas, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul (UFRGS), Rua Ramiro Barcelos, 2600 Anexo, Bairro Santa Cecília, CEP 90035-003, Porto Alegre, RS, Brazil

    Stephanie S. Silveira, Cesar E. J. Moritz & Emerson A. Casali

  7. Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, UFRGS, Rua Ramiro Barcelos 2600, Anexo, Bairro Santa Cecília, CEP 90035-003, Porto Alegre, RS, Brazil

    Emerson A. Casali

  8. Laboratório de Diagnóstico de Leptospirose, Universidade Federal de Santa Maria, Prédio 20, Sala 4235, Santa Maria, RS, Brazil

    Alexandre A. Tonin

Authors
  1. Alexandre A. Tonin
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  2. Aleksandro S. Da Silva
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  3. Jeandre A. Jaques
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  4. Viviane C. G. Souza
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  5. Paula T. Tonin
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  6. Camila Tochetto
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  7. Daniela B. R. Leal
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  8. Claudio A. M. Leal
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  9. Stephanie S. Silveira
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  10. Cesar E. J. Moritz
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  11. Emerson A. Casali
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  12. Sonia T. A. Lopes
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Correspondence to Alexandre A. Tonin.

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Tonin, A.A., Da Silva, A.S., Jaques, J.A. et al. Changes in serum levels of purines in an experimental infection by Leptospira interrogans serovar Icterohaemorrhagiae. Comp Clin Pathol 24, 721–725 (2015). https://doi.org/10.1007/s00580-014-1970-2

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  • DOI: https://doi.org/10.1007/s00580-014-1970-2

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