Abstract
Blood samples were collected from 170 cultured African sharptooth catfish (Clarias gariepinus) to establish haematological baseline values of this important tropical pisciculture fish species in the Guinea savannah agro-ecological zone of Nigeria. The total red blood cell count and the total white blood cell count were obtained by haemocytometry, while the packed cell volume and haemoglobin were obtained by microhaematocrit and cyanomethmoglobin methods, respectively. The results obtained varied between gender and age and were as follows: total red blood cell count, 1.72 ± 0.34 × 106/mm3 in juveniles and 4.50 ± 0.57 × 106/mm3 in adults; total white blood cell count, 15.50 ± 1.15103/mm3in juvenile and 16.41 ± 1.21 × 103/mm3in adults; packed cell volume, 30.08 ± 7.78 % in juveniles and 39.59 ± 3.93 % in adults; haemoglobin, 9.43 ± 3.45 g/dl in juveniles and 10.99 ± 3.29 g/dl in the adults; mean corpuscular volume, 173.75 ± 41.93 fl in juveniles and 87.01 ± 14.37 fl in adults; mean corpuscular haemoglobin, 51.11 ± 13.10 pg in the juveniles and 26.81 ± 8.61 pg in the adults, while the mean corpuscular haemoglobin concentration values obtained are 32.61 ± 10.42 g/dl in the juveniles and 33.80 ± 10.0 g/dl in the adults.
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References
Adedeji OB, Adegbile AF (2011) Comparative haematological parameters of the bagrid catfish (Chysichthys nigodigitatus) and the African catfish (Clarias gariepinus) from Asijire Dam in south western Nigeria. J Appl Sci Res 7(7):1042–1046
Adeyemo OK, Agbede SA, Olaniyan A, Shoaga OA (2003) The haematological response of Clarias gariepinus to changes in acclimation temperature. Afr J Biomed Res 6:105–108
Angelica D, Criteavsiulia G, Andlorera D (2010) Haematological response of the European catfish, (Silurus glanis) reared at different densities in flow through production systems. Archiva Zootechnica 13(2):63–70
Anver CE (2004) Blood chemistry (electrolytes; lipoprotein and enzymes) values of black scorpion fish (Scorpaena porcus, 1758) in the Dardanelles, Turkey. J Boil Sci 4:716–719
Arnandova A, Velchera I, Tomova E (2009) Changes in the erythrocyte indexes of Carassius gibelio (Pisces, Cyprinidea) under the influence of zinc. Biotechnology and Biotechnological Research. EQ 2009/SE
Barton BA (2000) Salmonid fishes differ in their cortisol and glucose responses to handling and transport stress. North Am J Aquac 62:218
Boyd CE, Tucker CS (1992) Water quality and soil analysis for aquaculture. Bulletin of the Agriculture Experiment Station, Auburn University, Auburn, Alabama US; 183 pp
Carrasco S, Sumano H, Navohro-Fierro R (1984) The use of lidocaine–sodium bicarbonate as an anesthetic in fish. Aquaculture 41:161–163
Chavez S, Martinez P, Osorio M, Palacios CAM, Mareno IO (1994) Pathological effects of feeding young Oreochromis nilocticus diets supplemented with different levels of aflatoxin. Aquaculture 127(1):49–61
Duncan JR, Prasse KW, Malaffey EA (1994) Veterinary laboratory medicine, 3rd edn. Iowa State University Press, Ames, pp 112–135
Ellis JG (1977) Hematology and clinical chemistry of cyprinid fish: common carp and goldfish. Vet Clin N Am Exotic Anim Pract 2(3):741–746
Ezeri GNO (2001) Hematological responses of Clarias gariepinus to bacterial infection and prophylactic treatment with antibiotics. J Aquat Sci 16:22–24
Federal Ministry of Agriculture and Rural Development of Nigeria (FMARD) (2003) Aquaculture development in: presidential forum on the fisheries development subsector, Federal Department of Fisheries. Fed Minist Agric Rural Dev 2003:68
Gabriel UU, Ezeri GNO, Opabunmi OO (2004) Influence of sex, source, health status and acclimations on the hematology of Clarias gariepinus (Buchel, 1822). Afr J Biotechnol 3(9):463–470
Gul Y, Gao ZX, Qian XQ, Wang WM (2011) Haematological and serum biochemical characterization and comparison of wild and cultured northern snakehead (Channa argus Cantor, 1842). J Appl Ichthyol 27(1):122–128
Huffman PA, Arkoosh MR, Casillas E (1997) Characteristics of peripheral blood cells from rainbow trout evaluated by particle counter image analysis and haemocytometric techniques. J Aquat Anim Heal 9:239–248
Kori-Siakpere O, Ake JEG, Idoge E (2005) Haematological characteristics of the African snakehead, Parachanna obscura. Afr J Biotechnol 4:527–530
Linnet K (2000) Non parametric estimation of refrence intervals by simple and bootstrap-based procedures. Clin Chem 46:869–869
Map of Nigeria showing Lower Benue River Basin (2010) http://www.enwikipedia.org/wiki/Benue_River. Accessed 22 Sep 2010
National Committee for Clinical Laboratory Standards (NCCLS) (1992) How to define, determine and utilize reference intervals in the clinical laboratory; proposed guidelines. Document C. NCCLS, Villanova, p 28
Noga EJ (2010) Fish diseases: diagnosis and treatment. Mosby, St. Louis, pp 226–227
Post L (1993) Aquaculture for veterinarians, fish husbandry and medicine. Butterworth, North Chicago, p 440
Rainza-Paiva MJT, Ishikawa CM, Das-Eiras AA, Felizardo NN (2000) Hematological analysis of class Pseudoplatystoma fasciatum in captivity. Aqua 2000. Responsible aquaculture in the new millennium. Nice, France. May 2–6, 2000. Eur Aquacult Soc 28:590, Special publication
Ruane NM, Nolan DT, Rotllant J, Costelloe J, Wendelaar Bonga SE (2000) Experimental exposure of rainbow trout Oncorhynchus mykiss (Walbaum) to the infective stages of the sea louse Lepeophtheirus salmonis (Kroyer) influences the physiological response to an acute stressor. Fish Shellfish Immunol 10:451–463
Sabiri DM, El-Danasoury MAEH, Eissa IAE-M, Khouraiba HM (2009) Impact of heneguyosis infestation on haematological parameters of catfish (Clarias gariepinus). Int J Agric Biol 11:228–230
Schalm OW, Jain NC, Carrol EJ (1975) Veterinary haematology, 3rd edn. Lea & Febinger, Philadelphia, pp 19–25
Schmitt CJ, Blazer VS, Dethloff GM, Tillitt DE, Gross TS, Brant WL Jr, DeWeese LR, Smith SB, Goede RW, Bartish TM, Kubiak T (1999) Biomonitoring of Environmental Status and Trends (BEST) program. Field procedures for assessing the exposure of fish to environmental contaminants. Information and Technology Report USGS/BRD-1999-0007. U.S. Geological Survey, Biological Resources Division, Columbia, 68 pp.
Shimura S, Inoue K, Kasai K, Saito H (1983) Haematolgical changes of Oncorhychus masou (Salmonidae) caused by the infection of Argulus coregoni (Crutacea: Branchiura). Fish Pathol 18:157–162
Stoskopf MK (1993) Fish medicine. College of Veterinary Medicine North Carolina State University, Raleigh, p 882
Tacon AGJ (1992) Nutritional fish pathology. Morphological signs of nutrients deficiency and toxicity in farmed fish. FAO fish technical paper no. 330. Rome. 75 p
Tavarez-Dias M, Moraes FR (2007) Haematological and biochemical reference intervals for farmed channel catfish. J Fish Biol 7:283–388
VanVuren JHJ (1986) The effects of toxicants on the haematology of Labeo umbratus (Teleostei: Cyprinidae). Comp Biochem Physiol 83C:155–159
Wagner T, Congleton JL (2004) Blood chemistry correlates of nutritional condition, tissue damage and stress in migrating juvenile Chinook salmon (Oncorhynchus tshawytscha). Can J fish Aqual Sci 61:1066–1074. doi:10:1139/f04-050
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Adeyemo, B.T., Obande, R.A. & Solomon, S.G. Haematological reference ranges of cultured Clarias gariepinus in the Lower Benue River Basin, Nigeria. Comp Clin Pathol 23, 361–366 (2014). https://doi.org/10.1007/s00580-012-1624-1
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DOI: https://doi.org/10.1007/s00580-012-1624-1