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Coagulase gene-based typing of Staphylococcus aureus from mastitic cattle and goats from arid region in India

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Abstract

Staphylococcus aureus is an important pathogen and causes mastitis and many other infections in animals as well as in humans. In the present study, S. aureus isolates were investigated for any variations based on coa gene polymorphism and AluI restriction fragment length polymorphism of coa amplicons. Thirty S. aureus isolates obtained from cattle and goats with clinical mastitis were identified by their phenotypic properties and then confirmed genotypically by PCR where all the isolates produced species-specific amplicon of 1,250 bp. Amplification of the coa gene produced products of three different sizes (600, 680, or 850 bp), one specific to each isolate. The strains of S. aureus were common to both cattle and goat, but the predominance of S. aureus isolates with coa gene amplicon of 680 bp was observed in cattle and 600 bp coa gene amplicon in goats. No difference in the restriction fragment length polymorphism (RFLP) patterns of coa amplicons with AluI endonuclease was obtained in isolates from cattle or goats, and there was limited strain variations in the region under study. The coa amplicons and RFLP patterns of isolates were similar to those recorded elsewhere.

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References

  • Aarestrup FM, Wegener HC, Jensen NE, Jonsson O, Myllys V, Thorberg BM, Waage S, Rosdahl VT (1997) A study of phage- and ribotype patterns of Staphylococcus aureus isolated from bovine mastitis in the Nordic countries. Acta Vet Scand 38:243–252

    PubMed  CAS  Google Scholar 

  • Aires-de-Sousa M, Parente ESR, da-Motta OV, Bonna ICF, Silva DA, de Lencastre H (2007) Characterization of Staphylococcus aureus isolates from buffalo, bovine, ovine and caprine milk samples collected in Rio de Janeiro state, Brazil. Appl Environ Microbiol 73:3845–3849

    Article  PubMed  CAS  Google Scholar 

  • Aslantas Ö, Demir C, Türütoğlu H, Cantekin Z, Ergün Y, Doğruer G (2007) Coagulase gene polymorphism of Staphylococcus aureus isolated from subclinical bovine mastitis. Turk J Vet Anim Sci 31:253–257

    CAS  Google Scholar 

  • da Silva ER, da Silva N (2005) Coagulase gene typing of Staphylococcus aureus isolated from cows with mastitis in southeastern Brazil. Can J Vet Res 69:260–264

    PubMed  Google Scholar 

  • da Silva ER, Boechat JUD, da Silva N (2006) Coagulase gene polymorphism of Staphylococcus aureus isolated from goat mastitis in Brazilian dairy herds. Lett Appl Microbiol 42:30–34

    Article  PubMed  CAS  Google Scholar 

  • Fitzgerald JR, Meaney WJ, Hartigan PJ, Smyth CJ, Kapur V (1997) Fine-structure molecular epidemiological analysis of Staphylococcus aureus recovered from cows. Epidemiol Infect 119:261–269

    Article  PubMed  CAS  Google Scholar 

  • Goh SH, Byrne SK, Zhang JJ, Chow AW (1992) Molecular typing of Staphylococcus aureus on the basis of coagulase gene polymorphism. J Clin Microbiol 30:1642–1645

    PubMed  CAS  Google Scholar 

  • Güler L, Ok Ü, Gündüz K, Gülcü Y, Hadimli HH (2005) Antimicrobial susceptibility and coagulase gene typing of Staphylococcus aureus isolated from bovine clinical mastitis in Turkey. J Dairy Sci 88:3149–3154

    Article  PubMed  Google Scholar 

  • Hookey JV, Richardson JF, Cookson BD (1998) Molecular typing of Staphylococcus aureus based on PCR restriction fragment length polymorphism and DNA sequence analysis of the coagulase gene. J Clin Microbiol 36:1083–1089

    PubMed  CAS  Google Scholar 

  • Kaida S, Miyata T, Yoshizawa Y, Kawabata S, Morita T, Igarashi H, Iwanaga S (1987) Nucleotide sequence of the staphylocoagulase gene: its unique COOH-terminal 8 tandem repeats. J Biochem 102:1177–1186

    PubMed  CAS  Google Scholar 

  • Kalorey DR, Shanmugam Y, Kurkure NV, Chousalkar KK, Barbuddhe SB (2007) PCR-based detection of genes encoding virulence determinants in Staphylococcus aureus from bovine subclinical mastitis cases. J Vet Sci 8:151–154

    Article  PubMed  Google Scholar 

  • Karimuribo ED, Kusiluka LJ, Mdegela R, Kapaga AM, Sindato C, Kambarage MD (2005) Studies on mastitis, milk quality and health risks associated with consumption of milk from pastoral herds in Dodoma and Morogoro regions, Tanzania. J Vet Sci 6:213–221

    PubMed  Google Scholar 

  • Kobayashi N, Taniguchi K, Kojima K, Urasawa S, Uehara N, Omizu Y, Kishi Y, Yagihashi A, Kurokawa I (1995) Analysis of methicillin resistant and methicillin susceptible Staphylococcus aureus by a molecular typing method based on coagulase gene polymorphism. Epidemiol Infect 115:419–426

    Article  PubMed  CAS  Google Scholar 

  • Lawrence C, Cosseron M, Mimoz O, Brun-Buisson C, Costa Y, Samii K, Duval J, Leclercq L (1996) Use of coagulase gene typing method for detection of carrier of methicillin resistant Staphylococcus aureus. J Antimicrob Chemother 37:687–696

    Article  PubMed  CAS  Google Scholar 

  • Luong TT, Newell SW, Lee CY (2003) mgr, a novel global regulator in Staphylococcus aureus. J Bacteriol 185:3703–3710

    Article  PubMed  CAS  Google Scholar 

  • Mørk T, Tollersrud T, Kvitle B, Jørgensen J, Waage S (2005) Comparison of Staphylococcus aureus genotypes recovered from cases of bovine, ovine, and caprine mastitis. J Clin Microbiol 43:3979–3984

    Article  PubMed  Google Scholar 

  • Moroni P, Pisoni G, Vimercati C, Rinaldi M, Castiglioni B, Cremonesi P, Boettcher P (2005) Characterization of Staphylococcus aureus isolated from chronically infected dairy goats. J Dairy Sci 88:3500–3509

    Article  PubMed  CAS  Google Scholar 

  • O’Riordan K, Lee JC (2004) Staphylococcus aureus capsular polysaccharides. Clin Microbiol Rev 17:218–234

    Article  PubMed  Google Scholar 

  • Phonimdaeng P, O’Reilly M, Nowlan P, Bramley AJ, Foster TJ (1990) The coagulase of Staphylococcus aureus 8325-4. Sequence analysis and virulence of site-specific coagulase-deficient mutants. Mol Microbiol 4:393–404

    Article  PubMed  CAS  Google Scholar 

  • Quinn PJ, Carter ME, Markey BK, Carter GR (1994) Clinical veterinary microbiology. Mosby International Microbiology, London, 118–126

  • Saei HD, Ahmadi M, Mardani K, Batavani RA (2009) Molecular typing of Staphylococcus aureus isolated from bovine mastitis based on polymorphism of the coagulase gene in the north west of Iran. Vet Microbiol 137:202–206

    Article  PubMed  CAS  Google Scholar 

  • Saei HD, Ahmadi M, Mardani K, Batavani RA (2010) Genotyping of Staphylococcus aureus isolated from bovine mastitis based on PCR-RFLP analysis of the aroA gene. Comp Clin Pathol 19:163–168

    Article  CAS  Google Scholar 

  • Salasia SI, Khusnan Z, Lamme RC, Zschock M (2004) Comparative studies on pheno- and genotypic properties of Staphylococcus aureus isolated from bovine subclinical mastitis in central Java in Indonesia and Hesse in Germany. J Vet Sci 5:103–109

    PubMed  Google Scholar 

  • Sambrook J, Fritsch EF, Maniatis T (1989) Purification of DNA. In: Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor

  • Sanjiv K, Kataria AK, Sharma R, Singh G (2008) Epidemiological typing of Staphylococcus aureus by DNA restriction fragment length polymorphism of coa gene. Vet Arhiv 78:31–38

    CAS  Google Scholar 

  • Schwarzkopf A, Karch H (1994) Genetic variation in Staphylococcus aureus coagulase genes. Potential and limits for use as epidemiological marker. J Clin Microbiol 32:2407–2412

    PubMed  CAS  Google Scholar 

  • Straub JA, Hertel C, Hammes WP (1999) A 23S rDNA-targeted polymerase chain reaction-based system for detection of Staphylococcus aureus in meat starter cultures and dairy products. J Food Prot 62:1150–1156

    PubMed  CAS  Google Scholar 

  • Su C, Herbelin C, Frieze N, Skardova O, Sordillo M (1999) Coagulase gene polymorphism of Staphylococcus aureus isolates from dairy cattle in different geographical areas. Epidemiol Infect 122:329–336

    Article  PubMed  CAS  Google Scholar 

  • Tollersrud T, Kenny K, Reitz AJ Jr, Lee JC (2000) Genetic and serologic evaluation of capsule production by bovine mammary isolates of Staphylococcus aureus and other Staphylococcus spp. from Europe and the United States. J Clin Microbiol 38:2998–3003

    PubMed  CAS  Google Scholar 

  • van Belkum A, Kluytmans J, Leeuwen WV, Bax R, Quint W, Peters E, Fluit AD, Vandenbroucke-Grauls C, Van den Brule A, Koeleman H, Melchers W, Meis J, Elaichouni A, Vaneechoutte M, Moonens F, Maes N, Struelens M, Tenover F, Verbrugh H (1995) Multicenter evaluation of arbitrarily primed PCR for typing of Staphylococcus aureus Strains. J Clin Microbiol 33:1537–1547

    PubMed  Google Scholar 

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Acknowledgments

We are thankful to the Dean, College of Veterinary and Animal Science and In-Charge, Plant Biotechnology Centre, S.K. Rajasthan Agricultural University, Bikaner for the facilities provided to accomplish the work.

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Authors and Affiliations

  1. Department of Veterinary Microbiology and Biotechnology, College of Veterinary and Animal Science, Rajasthan University of Veterinary and Animal Sciences, Bikaner, 334 001, Rajasthan, India

    Abhinav Upadhyay & Anil Kumar Kataria

  2. Plant Biotechnology Centre, S.K. Rajasthan Agricultural University, Bikaner, Rajasthan, India

    Ramavtar Sharma

  3. 125 Kailashpuri, Bikaner, 334 001, Rajasthan, India

    Anil Kumar Kataria

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  1. Abhinav Upadhyay
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  2. Anil Kumar Kataria
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  3. Ramavtar Sharma
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Correspondence to Anil Kumar Kataria.

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Upadhyay, A., Kataria, A.K. & Sharma, R. Coagulase gene-based typing of Staphylococcus aureus from mastitic cattle and goats from arid region in India. Comp Clin Pathol 21, 605–610 (2012). https://doi.org/10.1007/s00580-010-1141-z

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