Comparative Clinical Pathology

, Volume 12, Issue 3, pp 169–171

Determination of D-lactate in calf serum samples – an automated enzymatic assay

Authors

  • I. Lorenz
    • Second Medical Animal ClinicUniversity of Munich
  • I. Hartmann
    • Second Medical Animal ClinicUniversity of Munich
  • A. Gentile
    • Dipartimento Clinico VeterinarioUniversity of Bologna
Article

DOI: 10.1007/s00580-003-0499-6

Cite this article as:
Lorenz, I., Hartmann, I. & Gentile, A. Comp Clin Path (2003) 12: 169. doi:10.1007/s00580-003-0499-6

Abstract

Since recent research has shown that D-lactataemia is obviously not an unusual problem in calves, determination of this metabolite may have considerable clinical significance. In analogy to the routinely used L-lactate assay, a stereospecific enzymatic assay of D-lactate was automated on a Hitachi Automatic Analyzer 911. As the method described does not require deproteinisation, and amounts of reagents are small, the determination is inexpensive and not labour-intensive. The method proved to be both accurate and reliable, with a limit of linearity of 16 mmol/l. A reference range was established using serum samples from 150 clinically healthy Simmental calves of both sexes, up to 3 weeks old, from 48 dairy farms in southern Germany. An upper limit of the reference range (95th percentile) of 3.96 mmol/l was found.

Keywords

Automated enzymatic assay Calves D-lactate Reference values

Copyright information

© Springer-Verlag London Limited 2003