Original Paper

Mycorrhiza

, Volume 24, Issue 1, pp 35-43

Symbiotic seed germination and protocorm development of Aa achalensis Schltr., a terrestrial orchid endemic from Argentina

  • Fracchia SebastiánAffiliated withCentro Regional de Investigaciones Científicas y Transferencia Tecnológica, CRILAR- CONICET Email author 
  • , Silvani VanesaAffiliated withDepartamento de Biodiversidad y Biología Experimental. Facultad de Ciencias Exactas y Naturales (FCEyN), Universidad de Buenos Aires (UBA)
  • , Flachsland EduardoAffiliated withInstituto de Botánica del Nordeste (CONICET—Consejo Nacional de Investigaciones Científicas y Técnicas), Facultad de Ciencias Agrarias (UNNE—Universidad Nacional del Nordeste)
  • , Terada GracielaAffiliated withInstituto de Botánica del Nordeste (CONICET—Consejo Nacional de Investigaciones Científicas y Técnicas), Facultad de Ciencias Agrarias (UNNE—Universidad Nacional del Nordeste)
  • , Sede SilvanaAffiliated withInstituto de Botánica Darwinion

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access

Abstract

Aa achalensis is an endangered terrestrial orchid endemic from Argentina. In vitro symbiotic seed germination was evaluated for its propagation. Five different fungal strains were isolated from this species: two Rhizoctonia-like related to Thanatephorus cucumeris and three ascomicetaceous fungi belonging to Phialophora graminicola and one to an uncultured Pezizaceae. All five isolates promoted seed germination being one T. cucumeris strain the most effective. After 16 weeks of growth, 30 % of A. achalensis protocorms developed until seedlings with two/four leaves in this treatment. These findings open an opportunity to the knowledge and preservation of this species.

Keywords

Orchid mycorrhiza Seed germination Dark septate endophytes Conservation