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Bio-MEMS fabricated artificial capillaries for tissue engineering

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Abstract

In this report, we focus on the microfabrication and cell seeding issues of artificial blood capillaries for tissue engineering. Two different fabrication methods (stainless steel electroforming and silicon electroforming) and a number of materials (PC, Polycarbonate and biocompatible material PLGA, poly lactide-co-glycolides) are implemented to build the vascular network. The vascular network is then used as the scaffold to cultivate the bovine endothelial cell (BEC). During the period of cell cultivation, oxygen and nutrient need to be continuously delivered by a circular pressurizing system. In cell culture, encouraging results are obtained through the dynamical seeding of the BEC on the scaffolds. A systematic cell culture process has been developed after repeated experiments. Successful seeding efficiencies are obtained by using the developed systematic cell culture process.

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Acknowledgements

The authors would like to address their gratitude to the National Science Council of Taiwan for financial support under grant NSC-91-2212-E-005-012. The work was conducted in the Center of Tissue Engineering and Stem Cells Research (TESC) at the National Chung-Hsing University, Taiwan. The center is funded by National Health Research Institutes (NHRI).

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Authors and Affiliations

  1. Department of Mechanical Engineering, National Chung-Hsing University, Taichung, 402, Taiwan

    G. J. Wang & C. L. Chen

  2. Department of Chemical Engineering, National Chung-Hsing University, Taichung, 402, Taiwan

    S. H. Hsu & Y. L. Chiang

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  1. G. J. Wang
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  2. C. L. Chen
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  3. S. H. Hsu
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  4. Y. L. Chiang
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Correspondence to G. J. Wang.

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Wang, G.J., Chen, C.L., Hsu, S.H. et al. Bio-MEMS fabricated artificial capillaries for tissue engineering. Microsyst Technol 12, 120–127 (2005). https://doi.org/10.1007/s00542-005-0017-7

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  • DOI: https://doi.org/10.1007/s00542-005-0017-7

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