Journal of Gastroenterology

, Volume 47, Issue 12, pp 1298–1307

Multicenter analysis of fecal microbiota profiles in Japanese patients with Crohn’s disease

Authors

    • Division of Mucosal ImmunologyGraduate School, Shiga University of Medical Science
  • Hiroyuki Kuzuoka
    • Department of Lower GastroenterologyHyogo College of Medicine
    • Research and Development LaboratoriesEN Otsuka Pharmaceutical Co., Ltd.
  • Tomoyuki Tsujikawa
    • Department of MedicineShiga University of Medical Science
  • Shiro Nakamura
    • Department of Lower GastroenterologyHyogo College of Medicine
  • Fumihito Hirai
    • Department of GastroenterologyFukuoka University Chikushi Hospital
  • Yasuo Suzuki
    • Department of Internal MedicineToho University Sakura Medical Center
  • Toshiyuki Matsui
    • Department of GastroenterologyFukuoka University Chikushi Hospital
  • Yoshihide Fujiyama
    • Department of MedicineShiga University of Medical Science
  • Takayuki Matsumoto
    • Department of Lower GastroenterologyHyogo College of Medicine
Original Article—Alimentary Tract

DOI: 10.1007/s00535-012-0605-0

Cite this article as:
Andoh, A., Kuzuoka, H., Tsujikawa, T. et al. J Gastroenterol (2012) 47: 1298. doi:10.1007/s00535-012-0605-0

Abstract

Background

We analyzed the fecal microbiota profiles of patients with Crohn’s disease (CD) at 4 inflammatory bowel disease (IBD) centers located in different districts in Japan.

Methods

Terminal restriction fragment length polymorphism (T-RFLP) analysis was performed in 161 fecal samples from CD patients and 121 samples from healthy individuals. The bacterial diversity was evaluated by the Shannon diversity index (SDI).

Results

There were no regional differences in the fecal microbiota profiles of the healthy individuals in Japan. A setting of similarity generated three major clusters of T-RFs: one included almost all the healthy individuals (118/121), and the other two clusters were mainly formed by CD patients at different stages of disease activity. The changes in simulated bacterial composition indicated that the class Clostridia, including the genus Faecalibacterium, was significantly decreased in CD patients with active disease and those in remission as compared with findings in the healthy individuals. In contrast, the genus Bacteroides was significantly increased in CD patients during the active phase as compared with findings in the healthy individuals. The genus Bifidobacterium was significantly decreased during the active phase of CD and increased to healthy levels during the remission phase. The bacterial diversity measured by the SDI was significantly reduced in CD patients during the active and remission phases as compared with findings in the healthy individuals. From the clinical data and T-RFLP analysis, we developed a logistic model to predict disease activity based on the fecal microbiota composition.

Conclusion

Dysbiosis in CD patients was shown by a multi-IBD center study. The feasibility of using the fecal microbiota profile as a predictive marker for disease activity is proposed.

Keywords

Inflammatory bowel diseaseFaecalibacteriumFirmicutesShannon diversity indexT-RFLP

Supplementary material

535_2012_605_MOESM1_ESM.pptx (238 kb)
Supplementary material 1 (PPTX 237 kb)

Copyright information

© Springer 2012