Journal of Gastroenterology

, Volume 46, Issue 4, pp 479–486

Comparison of the fecal microbiota profiles between ulcerative colitis and Crohn’s disease using terminal restriction fragment length polymorphism analysis

  • Akira Andoh
  • Hirotsugu Imaeda
  • Tomoki Aomatsu
  • Osamu Inatomi
  • Shigeki Bamba
  • Masaya Sasaki
  • Yasuharu Saito
  • Tomoyuki Tsujikawa
  • Yoshihide Fujiyama
Original Article—Alimentary Tract

DOI: 10.1007/s00535-010-0368-4

Cite this article as:
Andoh, A., Imaeda, H., Aomatsu, T. et al. J Gastroenterol (2011) 46: 479. doi:10.1007/s00535-010-0368-4

Abstract

Background

Terminal restriction fragment length polymorphism (T-RFLP) analysis is a powerful tool to assess the diversity of a microbial community. In this study, we performed T-RFLP analysis of the fecal microbiota from patients with ulcerative colitis (UC) and those with Crohn’s disease (CD).

Methods

Thirty-one patients with UC, 31 patients with CD, and 30 healthy individuals were enrolled. The polymerase chain reaction (PCR) products obtained from the 16S rRNA genes of fecal samples were digested with BslI, and T-RF lengths were determined.

Results

The fecal microbial communities were classified into 5 clusters. Twenty-eight of the 30 healthy individuals and 17 of the 18 patients with inactive UC were classified into clusters I, II, and III, but these clusters included a small number of patients with active UC and inactive/active CD. In contrast, 8 of the 13 patients with active UC and the majority of CD patients (12 of the 16 patients with inactive CD, and 11 of the 15 patients with active CD) were included in clusters IV and V. Based on the BslI-digested T-RFLP database, the bacteria showed a significant decrease in the Clostridium family in patients with active UC and inactive/active CD. In contrast, Bacteroides were significantly increased in CD patients. No significant differences were observed between patients with active UC and those with active CD.

Conclusion

The fecal microbial communities of IBD patients were different from those of healthy individuals. The gut microbiota of patients with inactive UC tended to be closer to that of healthy individuals, suggesting different roles for the fecal microbiota in the pathophysiology of UC and CD.

Keywords

Inflammatory bowel diseaseT-RFLPClostridiumBacteroides

Copyright information

© Springer 2011

Authors and Affiliations

  • Akira Andoh
    • 1
  • Hirotsugu Imaeda
    • 2
  • Tomoki Aomatsu
    • 2
  • Osamu Inatomi
    • 2
  • Shigeki Bamba
    • 2
  • Masaya Sasaki
    • 2
  • Yasuharu Saito
    • 2
  • Tomoyuki Tsujikawa
    • 2
  • Yoshihide Fujiyama
    • 2
  1. 1.Division of Mucosal ImmunologyGraduate School of Medicine, Shiga University of Medical ScienceOtsuJapan
  2. 2.Department of MedicineShiga University of Medical ScienceOtsuJapan