Journal of Gastroenterology

, Volume 44, Issue 7, pp 793–798

Expression of OATP1B3 determines uptake of Gd-EOB-DTPA in hepatocellular carcinoma

  • Masato Narita
  • Etsuro Hatano
  • Shigeki Arizono
  • Aya Miyagawa-Hayashino
  • Hiroyoshi Isoda
  • Koji Kitamura
  • Kojiro Taura
  • Kentaro Yasuchika
  • Takashi Nitta
  • Iwao Ikai
  • Shinji Uemoto
Rapid Communication

DOI: 10.1007/s00535-009-0056-4

Cite this article as:
Narita, M., Hatano, E., Arizono, S. et al. J Gastroenterol (2009) 44: 793. doi:10.1007/s00535-009-0056-4

Abstract

Background

Gadolinium-ethoxybenzyl-diethylenetriamine pentaacetic acid (Gd-EOB-DTPA) is an MRI contrast agent with perfusion and hepatoselective properties. The purpose of the study was to examine uptake of Gd-EOB-DTPA in the hepatobiliary phase in hepatocellular carcinoma (HCC).

Methods

A retrospective analysis of 22 patients with HCC who underwent preoperative Gd-EOB-DTPA-enhanced MRI was performed. Enhancement ratios (ERs) and expression levels of the organic anion transporter (OATP) 1B3 protein were examined.

Results

Gd-EOB-DTPA accumulated in the hepatobiliary phase in 6 of the 22 cases. All 6 Gd-EOB-DTPA-positive cases were moderately differentiated HCC, but 11 other moderately differentiated HCCs did not show Gd-EOB-DTPA uptake. Histopathologically, 4 Gd-EOB-DTPA-positive HCCs and 5 Gd-EOB-DTPA-negative HCCs produced bile. HCCs with Gd-EOB-DTPA uptake overexpressed OATP1B3 compared with HCCs without Gd-EOB-DTPA uptake, and OATP1B3 levels were significantly correlated with ERs (r = 0.91, P < 0.0001).

Conclusions

Uptake of Gd-EOB-DTPA in HCC is determined by expression of OATP1B3 rather than by tumor differentiation or bile production.

Keywords

Green hepatomaGd-EOB-DTPAMRIOATP1B3HCC

Copyright information

© Springer 2009

Authors and Affiliations

  • Masato Narita
    • 1
  • Etsuro Hatano
    • 1
  • Shigeki Arizono
    • 2
  • Aya Miyagawa-Hayashino
    • 3
  • Hiroyoshi Isoda
    • 2
  • Koji Kitamura
    • 1
  • Kojiro Taura
    • 1
  • Kentaro Yasuchika
    • 1
  • Takashi Nitta
    • 1
  • Iwao Ikai
    • 1
  • Shinji Uemoto
    • 1
  1. 1.Department of Surgery, Graduate School of MedicineKyoto UniversityKyotoJapan
  2. 2.Department of Diagnostic Imaging and Nuclear Medicine, Graduate School of MedicineKyoto UniversityKyotoJapan
  3. 3.Department of Diagnostic PathologyKyoto University HospitalKyotoJapan