Original Article

Plant Reproduction

, Volume 26, Issue 3, pp 197-208

SGO1 but not SGO2 is required for maintenance of centromere cohesion in Arabidopsis thaliana meiosis

  • L. ZamariolaAffiliated withDepartment of Plant Production, Faculty of Bioscience Engineering, University of Ghent
  • , N. De StormeAffiliated withDepartment of Plant Production, Faculty of Bioscience Engineering, University of Ghent
  • , CL. TiangAffiliated withDepartment of Plant Breeding and Genetics, Cornell University
  • , S. J. ArmstrongAffiliated withSchool of Bioscience, University of Birmingham
  • , F. C. H. FranklinAffiliated withSchool of Bioscience, University of Birmingham
  • , D. GeelenAffiliated withDepartment of Plant Production, Faculty of Bioscience Engineering, University of Ghent Email author 

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Abstract

Shugoshin is a protein conserved in eukaryotes and protects sister chromatid cohesion at centromeres in meiosis. In our study, we identified the homologs of SGO1 and SGO2 in Arabidopsis thaliana. We show that AtSGO1 is necessary for the maintenance of centromere cohesion in meiosis I since atsgo1 mutants display premature separation of sister chromatids starting from anaphase I. Furthermore, we show that the localization of the specific centromeric cohesin AtSYN1 is not affected in atsgo1, suggesting that SGO1 centromere cohesion maintenance is not mediated by protection of SYN1 from cleavage. Finally, we show that AtSGO2 is dispensable for both meiotic and mitotic cell progression in Arabidopsis.

Keywords

Arabidopsis Meiosis Shugoshin Centromere cohesion