Original Article

Plant Reproduction

, Volume 26, Issue 3, pp 297-307

Open Access This content is freely available online to anyone, anywhere at any time.

Failure of androgenesis in Miscanthus × giganteus in vitro culture of cytologically unbalanced microspores

  • Iwona ŻurAffiliated withFranciszek Górski Institute of Plant Physiology, Polish Academy of Sciences
  • , Ewa DubasAffiliated withFranciszek Górski Institute of Plant Physiology, Polish Academy of Sciences Email author 
  • , Aneta SłomkaAffiliated withDepartment of Plant Cytology and Embryology, Jagiellonian University
  • , Franciszek DubertAffiliated withFranciszek Górski Institute of Plant Physiology, Polish Academy of Sciences
  • , Elżbieta KutaAffiliated withDepartment of Plant Cytology and Embryology, Jagiellonian University
  • , Agnieszka PłażekAffiliated withDepartment of Plant Physiology, University of Agriculture

Abstract

Miscanthus × giganteus is a popular energy crop, which due to its hybrid origin is only vegetatively reproduced. Asexual embryogenesis in anther and microspore culture leading to double haploids production could allow to regain the ability for sexual reproduction and to increase the biodiversity of the species. Therefore, the goal of this paper was to investigate the requirements of androgenesis in Miscanthus. The standard protocols used for monocotyledonous plants were applied with many modifications regarding the developmental stage of the explants at the time of culture initiation, stress treatment applied to panicles and isolated anthers as well as various chemical and physical parameters of in vitro culture conditions. Our results indicated that the induction of androgenesis in M. × giganteus is possible. However, the very low efficiency of the process and the lack of regeneration ability of the androgenic structures presently prevent the use of this technique.

Keywords

Anther culture Honey Meiotic abnormalities Microspore embryogenesis