Abstract
An experiment was conducted to determine whether, by using a low-protein amino acid-supplemented diet, the health status, stress response, and excreta quality could be improved without affecting the productive performance of heat-stressed laying hens. The requirements for egg production, egg mass, and feed conversion ratio were also estimated using second-order equations and broken-line regression. A total of 150 Lohmann Selected Leghorn (LSL-Lite) hens were divided randomly into five groups of 30 with five replicates of six hens. The hens were raised for an 8-week period (52 to 60 weeks) in wire cages situated in high ambient temperature in an open-sided housing system. The five experimental diets (ME; 2,720 kcal/kg) varied according to five crude protein (CP) levels: normal-CP diet (control, 16.5 % CP) and low-CP diets containing 15.0, 13.5, 12.0, or 10.5 % CP. All experimental diets were supplemented with crystalline amino acids at the levels sufficient to meet their requirements. The results showed that under high temperature conditions, all productive performance and egg quality parameters in the birds fed with 15.0, 13.5, and 12.0 % CP diets were similar to those of birds fed with control diet (16.5 % CP), whereas feeding 10.5 % CP diet significantly decreased egg production and egg mass. Estimations of requirements were of 13.93 and 12.77 % CP for egg production, 14.62 and 13.22 % CP for egg mass, and 12.93 and 12.26 % CP for feed conversion ratio using quadratic and broken-line models, respectively. Egg yolk color index, blood triglyceride level, and excreta acidity were also significantly higher in birds fed with 12.0 and 10.5 % CP diets compared with those of control birds. The heterophil to lymphocyte ratio, as a stress indicator, was significantly decreased by 15.0, 13.5, and 12 % CP diets. On the basis of our findings, reducing dietary CP from 16.5 to 12.0 % and supplementing the diets with the essential amino acids showed merit for improving the stress response and excreta quality while maintaining acceptable production performance from laying hens under high ambient temperature conditions.
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References
Adeyemo G, Abioye S, Aderemi F (2012) The effect of varied dietary crude protein levels with balanced amino acids on performance and egg quality characteristics of layers at first laying phase. Food Nutr Sci 3:526–529
Aengwanich W, Suttajit M (2010) Effect of polyphenols extracted from Tamarind (Tamarindus indica L.) seed coat on physiological changes, heterophil/lymphocyte ratio, oxidative stress and body weight of broilers (Gallus domesticus) under chronic heat stress. Anim Sci J 81:264–270
Bunchasak C, Silapasorn T (2005) Effects of adding methionine in low-protein diet on production performance, reproductive organs and chemical liver composition of laying hens under tropical conditions. Int J Poult Sci 4:301–308
Collett SR (2012) Nutrition and wet litter problems in poultry. Anim Feed Sci Technol 173:65–75
Deng W, Dong XF, Tong JM, Zhang Q (2012) The probiotic Bacillus licheniformis ameliorates heat stress-induced impairment of egg production, gut morphology, and intestinal mucosal immunity in laying hens. Poult Sci 91:575–582
Draper NR, Smith H (1981) Applied Regression Analysis, 2nd edn. John Wiley and Sons, New York
Eisen E, Bohren B, McKean H (1962) The Haugh unit as a measure of egg albumen quality. Poult Sci 41:1461–1468
Elliott HA, Collins NE (1982) Factors affecting ammonia release in broiler litter. Trans ASAE 25:413–418
Galassi G, Colombini S, Malagutti L, Crovetto GM, Rapetti L (2010) Effects of high fibre and low protein diets on performance, digestibility, nitrogen excretion and ammonia emission in the heavy pig. Anim Feed Sci Technol 161:140–148
Ghasemi HA, Ghasemi R, Torki M (2013) Periodic usage of low-protein methionine-fortified diets in broiler chickens under high ambient temperature conditions: effects on performance, slaughter traits, leukocyte profiles and antibody response. Int J Biometeorol. doi:10.1007/s00484-013-0741-0
Hempe J, Lauxen R, Savage J (1998) Rapid determination of egg weight and specific gravity using acomputerized data collection system. Poult Sci 67:902–907
Hsu JC, Lin CY, Chiou PWS (1998) Effects of ambient temperature and methionine supplementation of a low protein diet on the performance of laying hens. Anim Feed Sci Technol 74:289–299
Javaid S, Anjum MI, Akram M (2012) Effect of dietary protein and energy level on proximate composition of breast and thigh meat in white leghorn layers at molt and post molt production stages. Pak Vet J 32:483–488
Keshavarz K, Austic R (2004) The use of low-protein, low-phosphorus, amino acid-and phytase-supplemented diets on laying hen performance and nitrogen and phosphorus excretion. Poult Sci 83:75–83
Keshavarz K, Jackson ME (1992) Performance of growing pullets and laying hens fed low-protein, amino acid-supplemented diets. Poult Sci 71:905–918
Koelkebeck K, Parsons C, Leeper R, Wang X (1993) Effect of supplementation of a low-protein corn molt diet with amino acids on early postmolt laying hen performance. Poult Sci 72:1528–1536
Laudadio V, Dambrosio A, Normanno G, Khan RU, Naz S, Rowghani E, Tufarelli V (2012) Effect of reducing dietary protein level on performance responses and some microbiological aspects of broiler chickens under summer environmental conditions. Avian Biol Res 5:88–92
Leeson S (2006) Feeding programes for laying hens, Technical Report Series Poultry Nutrition and Management. American Soybean Association International Marketing Southeast Asia, Singapore, pp 56–75
Leeson S, Summers JD (2005) Commercial Poultry Nutrition, 3rd edn. Univ. Books, Guelph
Lin H, Jiao H, Buyse J, Decuypere E (2006) Strategies for preventing heat stress in poultry. World Poult Sci J 62:71–86
Liu Z, Wu G, Bryant MM, Roland DA (2005) Influence of added synthetic lysine in low-protein diets with the methionine plus cycteine to lysine ratio maintained at 0.75. J Appl Poult Res 14:174–182
Lucas A, Jamroz C (1961) Atlas of avian hematology. Agriculture Monograph, vol 25. USDA, Washington, DC
Mashaly MM, Hendricks GL, Kalama MA, Gehad AE, Abbas AO, Patterson PH (2004) Effect of heat stress on production parameters and immune responses of commercial laying hens. Poult Sci 83:889–894
Namroud N, Shivazad M, Zaghari M (2010) Impact of dietary crude protein and amino acids status on performance and some excreta characteristics of broiler chicks during 10–28 days of age. J Anim Physiol Anim Nutr 94:280–286
Novak CL, Yakout H, Scheideler SE (2004) The combined effects of dietary lysine and total sulfur amino acid level on egg production parameters and egg components in Dekalb Delta laying hens. Poult Sci 83:977–984
Novak C, Yakout H, Scheideler SE (2006) The effect of dietary protein level and total sulfur amino acid: lysine ratio on egg production parameters and egg yield in Hy-Line W-98 hens. Poult Sci 85:2195–2206
NRC (1994) Nutrient requirements of poultry, 9th edn. National Academy Press, Washington, DC
Penz AM, Jensen LS (1991) Influence of protein concentration, amino acid supplementation, and daily time of access to high-or low-protein diets on egg weight and components in laying hens. Poult Sci 70:2460–2466
Pesti G (2009) Impact of dietary amino acid and crude protein levels in broiler feeds on biological performance. J Appl Poult Res 18:477–486
Poosuwan K, Bunchasak C, Kaewtapee C (2010) Long-term feeding effects of dietary protein levels on egg production, immunocompetence and plasma amino acids of laying hens in subtropical condition. J Anim Physiol Anim Nutr 94:186–195
Quinteiro-Filho W, Ribeiro A, Ferraz-de-Paula V, Pinheiro M, Sakai M, Sá L, Ferreira A, Palermo-Neto J (2010) Heat stress impairs performance parameters, induces intestinal injury, and decreases macrophage activity in broiler chickens. Poult Sci 89:1905–1914
Robbins KR, Saxton AM, Southern LL (2006) Estimation of nutrient requirements using broken-line regression analysis. J Anim Sci 84(E Suppl):E155–E165
Roberts SA, Xin H, Kerr BJ, Russell JR, Bregendahl K (2007) Effects of dietary fiber and reduced crude protein on nitrogen balance and egg production in laying hens. Poult Sci 86:1716–1725
SAS (2001) Institute SAS user’s guide: statistics, 8th edn. SAS Institute Inc, Cary
Simkiss K, Taylor C (1957) A histochemical study of the organic matrix of hen egg-shells. Q J Microbiol Sci 98:19–28
Sohail MU, Hume ME, Byrd JA, Nisbet DJ, Ijaz A, Sohail A, Shabbir MZ, Rehman H (2012) Effect of supplementation of prebiotic mannan-oligosaccharides and probiotic mixture on growth performance of broilers subjected to chronic heat stress. Poult Sci 91:2235–2240
Summers JD (1993) Reducing nitrogen excretion of the laying hen by feeding lower crude protein diets. Poult Sci 72:1473–1478
Summers JD, Leeson S (1994) Laying hen performance as influenced by protein intake to sixteen weeks of age and body weight at point of lay. Poult Sci 73:495–501
Swennen Q, Janssens G, Millet S, Vansant G, Decuypere E, Buyse J (2005) Effects of substitution between fat and protein on feed intake and its regulatory mechanisms in broiler chickens: endocrine functioning and intermediary metabolism. Poult Sci 84:1051–1057
van der Hoeven-Hangoor E, Paton ND, van de Linde IB, Verstegen MW, Hendriks WH (2013) Moisture content in broiler excreta is influenced by excreta nutrient contents. J Anim Sci 91:5705–5713
Wolde S, Negesse T, Melesse A (2011) Effect of dietary protein concentration on feed intake, body mass gain and carcass traits of Rhode Island Red chicken. J Sci Dev 1:53–64
Yeh Y-Y, Leveille GA (1969) Effect of dietary protein on hepatic lipogenesis in the growing chick. J Nutr 98:356–366
Zaman QU, Mushtaq T, Nawaz H, Mirza MA, Mahmood S, Ahmad T, Babar ME, Mushtaq MMH (2008) Effect of varying dietary energy and protein on broiler performance in hot climate. Anim Feed Sci Technol 146:302–312
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This work was funded by Razi University (Kermanshah, Iran).
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Torki, M., Mohebbifar, A., Ghasemi, H.A. et al. Response of laying hens to feeding low-protein amino acid-supplemented diets under high ambient temperature: performance, egg quality, leukocyte profile, blood lipids, and excreta pH. Int J Biometeorol 59, 575–584 (2015). https://doi.org/10.1007/s00484-014-0870-0
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DOI: https://doi.org/10.1007/s00484-014-0870-0