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Estimation of genetic variability and population structure in Sapindus trifoliatus L., using DNA fingerprinting methods

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Abstract

Genetic variability and population structure of Sapindus trifoliatus L. (Sapindaceae), collected from Gujarat, Karnataka and Uttar Pradesh states were estimated using three DNA fingerprinting methods viz., random amplified polymorphic DNA (RAPD), directed amplification of minisatellite DNA (DAMD) and inter-simple sequence repeats (ISSR). The cumulative data analysis carried out for all three markers showed 69.42 % polymorphism. The intra-population genetic diversity analysis revealed the highest values of Nei’s genetic diversity (0.16), Shannon information index (0.24) and polymorphic loci (43.99 %) among Bhavnagar (BH) population, whereas lowest values were found in Junagarh (JU) population. The maximum inter-population average genetic distance (0.20) was between Allahabad (AL) and JU populations. Analysis of molecular variance (AMOVA) showed highest percentage of variation among individuals of populations (56 %) followed by 25 % among populations and 19 % among regions. Principal coordinate analysis and UPGMA dendrogram revealed that genetic diversity was in congruence with the geographical diversity. The data strongly suggest that low genetic flow, geographic isolation and to some extent genetic drift are the major factors responsible for high genetic differentiation. Preservation of genetic diversity of S. trifoliatus is important, both to promote adaptability of the populations to changing environment as well as to preserve a large gene pool for future genetic improvement. The present study using RAPD, DAMD and ISSR profiles of S. trifoliatus provide the means of rapid characterization of accessions within the populations, and thus enable the selection of appropriate accessions for further utilization in conservation and prospection programs of this important plant genetic resource.

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Abbreviations

AL:

Allahabad

BH:

Bhavnagar

HS:

Hassan

JU:

Junagarh

SH:

Shimoga

SR:

Sirsi

UP:

Uttar Pradesh

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Acknowledgments

We are thankful to the Director, CSIR-National Botanical Research Institute, Lucknow, India for facilities and encouragement. We are also thankful to Dr. R. R. Rao (Emeritus Scientist) for his help and unrelenting support during the collection of plant material from various locales of Western Ghats. This study was financially supported by the Department of Science and Technology, Government of India, New Delhi (DST No: SP/SOPS-81/05).

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Authors and Affiliations

  1. Molecular Systematics, CSIR-National Botanical Research Institute, Rana Pratap Marg, Lucknow, 226 001, Uttar Pradesh, India

    Kamalesh Singh Mahar & Tikam Singh Rana

  2. Plant Molecular Biology (Genomics) Laboratory, CSIR-National Botanical Research Institute, Rana Pratap Marg, Lucknow, 226 001, Uttar Pradesh, India

    Shirish Anand Ranade

  3. Department of Biotechnology, Kumaun University, Bhimtal, Nainital, 263 136, Uttarakhand, India

    Veena Pande

  4. G. B. Pant Institute of Himalayan Environment and Development, Kosi-Katarmal, Almora, 263 643, Uttarakhand, India

    Lok Man Singh Palni

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  1. Kamalesh Singh Mahar
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  2. Tikam Singh Rana
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Correspondence to Tikam Singh Rana.

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Communicated by F. Canovas.

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Mahar, K.S., Rana, T.S., Ranade, S.A. et al. Estimation of genetic variability and population structure in Sapindus trifoliatus L., using DNA fingerprinting methods. Trees 27, 85–96 (2013). https://doi.org/10.1007/s00468-012-0770-z

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  • DOI: https://doi.org/10.1007/s00468-012-0770-z

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