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Construction of a framework map for Pinus koraiensis Sieb. et Zucc. using SRAP, SSR and ISSR markers

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Abstract

Genetic linkage maps are essential for molecular breeding program. The first genetic linkage map of Pinus koraiensis, using an F1 progeny of 94 individuals, was constructed in the present paper. One hundred and twenty-two molecular markers were mapped onto 11 linkage groups, 1 triple and 8 pairs at the linkage criteria LOD 4.0. Among these markers, there were 96 sequence-related amplified polymorphism (SRAP) markers, 25 simple sequence repeat (SSR) markers, and 1 inter-simple sequence repeat (ISSR) marker. The consensus map gained covers 857.464 cM Kosambi (K) with an average marker spacing of 7.03 cM K. The presented map provides a basis and crucial information for future genomic studies of P. koraiensis, in particular for quantitative trait loci (QTL) mapping of economically important breeding target traits.

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Acknowledgments

This study was supported by the Knowledge Innovation Program of the Chinese Academy of Sciences (KZCX2-YW-416). The authors would like to thank Dr. Genyi Li (University of Manitoba, Canada) for providing them with the SRAP primer pairs. The authors also thank Dr. Cala Zelmer (University of Manitoba, Canada) for helpful comments on the manuscript.

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Authors and Affiliations

  1. Institute of Applied Ecology, Chinese Academy of Sciences, 72 Wenhua Road, Shenhe District, Shenyang, 110016, Liaoning, China

    Min-Min Chen, Xin Sui & Shijie Han

  2. Faculty of Life Science, Northeast Forestry University, Harbin, 150040, China

    Fujuan Feng & Dan Zhao

  3. Swiss Federal Research Institute WSL, Zurcherstrasse 111, 8903, Birmensdorf, Switzerland

    Mai-He Li

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  1. Min-Min Chen
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  2. Fujuan Feng
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  3. Xin Sui
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  4. Mai-He Li
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  5. Dan Zhao
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  6. Shijie Han
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Correspondence to Shijie Han.

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Communicated by R. Hampp.

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Chen, MM., Feng, F., Sui, X. et al. Construction of a framework map for Pinus koraiensis Sieb. et Zucc. using SRAP, SSR and ISSR markers. Trees 24, 685–693 (2010). https://doi.org/10.1007/s00468-010-0438-5

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  • DOI: https://doi.org/10.1007/s00468-010-0438-5

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