Original Paper


, Volume 23, Issue 3, pp 597-604

First online:

Effect of auxins on adventitious rooting from stem cuttings of candidate plus tree Pongamia pinnata (L.), a potential biodiesel plant

  • Vigya KesariAffiliated withDepartment of Biotechnology, Indian Institute of Technology Guwahati
  • , Anitha KrishnamachariAffiliated withCrop Research Informatics Laboratory, International Rice Research Institute
  • , Latha RanganAffiliated withDepartment of Biotechnology, Indian Institute of Technology Guwahati Email author 

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Pongamia pinnata, commercially important tree species used to produce biofuels, is known for its multipurpose benefits and its role in agro-forestry. Present study examines the amenability of vegetative propagation and effect of maturation in candidate plus tree P. pinnata through rooting of stem cuttings treated with varying concentrations and combinations of auxins. The performance of the cuttings was evaluated using SAS GLM software and the data were analyzed as a one-way classified data with and without sub sampling for inferring auxin concentration that can be included in programmes aimed at genetic improvement of the tree species. All auxin treatments promoted sprouting and at lower concentrations triggered/enhanced rooting of cuttings. The effectiveness was in the order of IBA > NAA > IAA when applied singly. IBA at 4.92 mM was found to be most effective where rooting percentage and number of roots were significantly higher (P < 0.01) than in control. However higher concentrations of auxins above 7 mM in general inhibited the rooting of cuttings. The interaction among auxins was found to be effective in root induction and differentiation and the most stimulating effects were observed in three-component mixture. The effect of other cutting characteristics such as juvenility and cutting position on rooting is also discussed.


Biodiesel Pongamia Plus tree Stem cuttings Vegetative propagation