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Two-photon in vivo imaging of cells

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Abstract

In vivo imaging of cells gives a glimpse into the world of biology in a natural setting unparalleled by any other venue. Two-photon imaging of fluorescently labeled cells has become the standard to obtain high-resolution, dynamic images of living specimens with great specificity. This review focuses on providing the reader with a short history of, and impetus behind, two-photon imaging, its working mechanics, and emerging technologies related to biological multiphoton imaging.

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Authors and Affiliations

  1. The Institute of Optics, University of Rochester, Rochester, NY, USA

    Daniel J. Christensen

  2. Center for Translational Neuromedicine, University of Rochester Medical Center, Rochester, NY, USA

    Daniel J. Christensen & Maiken Nedergaard

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  1. Daniel J. Christensen
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  2. Maiken Nedergaard
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Correspondence to Daniel J. Christensen.

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Christensen, D.J., Nedergaard, M. Two-photon in vivo imaging of cells. Pediatr Nephrol 26, 1483–1489 (2011). https://doi.org/10.1007/s00467-011-1818-9

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